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US4245042A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Device for harvesting cell cultures
Assignee: Yeda Research and Development Co Ltd
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Filing: 1979-01-22
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Granted: 1981-01-13
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Expires: 1999-01-22
Abstract
Abstract The present invention relates to a device for harvesting cells from a plurality of wells of a standard cell culture plate. Two complementary blocks, a lower and an upper one, are adapted to be attached with each other by suitable mechanical means to form a tight fit, the lower block being provided with a channel-formed conduit attached with an external source of a washing fluid, the flow of which is controlled by valve means. The conduit is connected with a plurality of downwardly pointing tubelets which are spaced in such manner that each one of them fits into a well of the culture plate, thus providing for the possibility of introducing at will washing fluid into the wells in the culture plate. Further tubes are provided which point in the same direction, each of which is connected with an outlet at the upper face of the lower block; an O-ring is provided substantially flush with the upper face of the block at each of the outlets. A central conduit is provided in the upper block, adapted to be connected with a vacuum source. The central conduit is connected via conduits connected therewith to each of the outlets at the upper face of the lower block, forming a tight connection via the O-rings. Cell cultures from a plurality of cells may be simultaneously harvested by means of this device.
Inventors
- 👤 Yacob Weinstein
- 👤 Jehoshua Wolowelsky
- 👤 Nurit Gideoni
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 13:17
CPC Classifications
C; C12; C12M; C12M33/00; C12M33/04; C12M33/14
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
277 words
A device for harvesting cells from a plurality of cells of a standard cell culture plate, comprising: an upper block; a lower block, complementary with said upper block; attaching means for clamping said upper and lower blocks together with a tight fit; first conduit means, provided within said lower block, for carrying washing fluid from a source thereof; valve means, connected to said first conduit means, for controlling the flow of washing fluid therein; a plurality of tubelets connected at the upper ends thereof to said first conduit means, the lower ends thereof extending below the bottom of said lower block, said tubelets being so spaced such that each of them fits into a well of the culture plate when in use; a plurality of tubes, parallel with said tubelets, passing through said lower block, one end of each of said tubes extending below said lower block and the other end of each of said tubes terminating at a respective one of a plurality of outlets at the upper face of said lower block; second conduit means, provided within said upper block, for connecting each of the outlets of said tubes in said lower block with a source of vacuum, said second conduit means including a plurality of openings in the lower face of said upper block, said openings being so spaced as to be opposite one of said outlets in said lower block when in use; and a plurality of sealing means, one of said sealing means being disposed at each of said outlets, for creating a tight connection between said outlets in said lower block and said openings in said upper block when in use.
Claim 2
Independent Claim
277 words
A device for harvesting cells from a plurality of cells of a standard cell culture plate, comprising: an upper block; a lower block, complementary with said upper block; attaching means for clamping said upper and lower blocks together with a tight fit; first conduit means, provided within said lower block, for carrying washing fluid from a source thereof; valve means, connected to said first conduit means, for controlling the flow of washing fluid therein; a plurality of tubelets connected at the upper ends thereof to said first conduit means, the lower ends thereof extending below the bottom of said lower block, said tubelets being so spaced such that each of them fits into a well of the culture plate when in use; a plurality of tubes, parallel with said tubelets, passing through said lower block, one end of each of said tubes extending below said lower block and the other end of each of said tubes terminating at a respective one of a plurality of outlets at the upper face of said lower block; second conduit means, provided within said upper block, for connecting each of the outlets of said tubes in said lower block with a source of vacuum, said second conduit means including a plurality of openings in the lower face of said upper block, said openings being so spaced as to be opposite one of said outlets in said lower block when in use; and a plurality of sealing means, one of said sealing means being disposed at each of said outlets, for creating a tight connection between said outlets in said lower block and said openings in said upper block when in use.
Claim 3
Dependent Claim
25 words
A device according to claim 1, further including a filter medium interposed between said lower and upper blocks, tightly secured therebetween by said attaching means.
Claim 4
Dependent Claim
14 words
A device according to claim 1, wherein said attaching means comprises screws and bolts.
Claim 5
Dependent Claim
15 words
A device in accordance with claim 1 wherein each said sealing means comprises an O-ring.
Claim 6
Dependent Claim
138 words
A method for the simultaneous harvesting of a plurality of individual cell cultures cultivated in the wells of a conventional microculture plate using the device in accordance with claim 1, comprising: inserting filter medium between said upper and lower blocks and clamping said blocks tightly together by means of said attaching means; positioning said device such that one of said tubelets and one of said tubes extend into each of the wells of the microculture plate; applying a vacuum to said second conduit means so as to aspirate the content of the wells to individual areas of said filter medium; introducing washing fluid into the wells by means of said first conduit means and said tubelets, which washing fluid is aspirated through said filter medium; disconnecting the vacuum; and removing the filter medium for subsequent treatment or analysis.
Claim 7
Dependent Claim
34 words
A method in accordance with claim 5 wherein each said sealing means comprises an O-ring, and each of the well contents on said filter medium is confined to an area surrounded by an O-ring.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-01-13 | 1984-07-14 | 1985-01-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-01-13 | 1988-07-14 | 1989-01-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-01-13 | 1992-07-14 | 1993-01-13 | $7700.00 | Window Closed / Lapsed |