US4243582A
Expired / Lapsed
US
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Novel glycoproteins from bovine cartilage
Assignee: Monsanto Co
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Filing: 1979-04-26
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Granted: 1981-01-06
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Expires: 1999-04-26
Abstract
Abstract Two high purity glycoprotein components each having a molecular weight of about 65,000 daltons and having activity as trypsin inhibitors and inhibitors of endothelial cell growth are obtained from bovine cartilage extract. A guanidine hydrochloride extract of bovine cartilage is subjected to ammonium sulfate salt fractionation, DEAE-Sephadex ion exchange chromatography, SP-Sephadex ion exchange chromatography and Con A-Sepharose affinity chromatography to isolate these glycoproteins in a high purity preparation.
Inventors
- 👤 Curtis A. Spilburg
- 👤 James M. Schuck
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 02:24
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/81; C07K14/8107; C07K14/811; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/84
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
151 words
A high purity glycoprotein isolated from bovine cartilage selected from the group consisting of Component A and Component B and having the following characteristics: (a) amino acid composition as follows: ______________________________________
Number of Residues
Amino Acid Component A Component B
______________________________________
LYS 25 19
HIS 10 9
ARG 13 12
ASP 39 35
THR 23 20
SER 31 24
GLU 47 40
PRO 28 23
GLY 22 19
ALA 34 28
1/2 CYS 10 4
VAL 31 27
MET 5 4
ILE 15 14
LEU 37 35
TYR 10 8
PHE 16 15
______________________________________ said number of residues for each amino acid being subject to a variation of about±10% from the number stated; (b) carbohydrate composition comprising hexose, fucose, sialic acid and hexosamine; (c) molecular weight of about 65,000±10,000; (d) isoelectric point of about 3.8; and (e) having activity as an inhibitor of endothelial cell growth and as a trypsin inhibitor.
Claim 2
Dependent Claim
151 words
A high purity glycoprotein isolated from bovine cartilage selected from the group consisting of Component A and Component B and having the following characteristics: (a) amino acid composition as follows: ______________________________________
Number of Residues
Amino Acid Component A Component B
______________________________________
LYS 25 19
HIS 10 9
ARG 13 12
ASP 39 35
THR 23 20
SER 31 24
GLU 47 40
PRO 28 23
GLY 22 19
ALA 34 28
1/2 CYS 10 4
VAL 31 27
MET 5 4
ILE 15 14
LEU 37 35
TYR 10 8
PHE 16 15
______________________________________ said number of residues for each amino acid being subject to a variation of about±10% from the number stated; (b) carbohydrate composition comprising hexose, fucose, sialic acid and hexosamine; (c) molecular weight of about 65,000±10,000; (d) isoelectric point of about 3.8; and (e) having activity as an inhibitor of endothelial cell growth and as a trypsin inhibitor.
Claim 3
Dependent Claim
58 words
A process of preparing the glycoproteins of claim 1 comprising the steps of (a) fractionating a guanadine .HCl extract of bovine cartilage with 40-80% ammonium sulfate; (b) adsorbing the fractionated substance with DEAE-Sephadex ion exchanger and desorbing by elution; (c) passing the eluate over SP-Sephadex ion exchanger; (d) passing over Con A-Sepharose; and (e) recovering the glycoproteins therefrom.
Claim 4
Dependent Claim
96 words
A process for preparing the glycoproteins of claim 1 comprising the steps of (a) fractionating a guanidine .HCl extract of bovine cartilage with 40-80% ammonium sulfate; (b) adsorbing the fractionated substance with DEAE-Sephadex ion exchanger in about 0.01 M NaCl, buffered at about pH 8.0, and desorbing by eluting with a substantially linear salt gradient to about 0.25 M NaCl; (c) passing the eluate over SP-Sephadex in about 50 mM sodium acetate, about pH 5.0; (d) passing over Con A-Sepharose in about 0.5 M NaCl, buffered at about pH 6.7; and (e) recovering the glycoproteins therefrom.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-01-06 | 1984-07-07 | 1985-01-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-01-06 | 1988-07-07 | 1989-01-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-01-06 | 1992-07-07 | 1993-01-06 | $7700.00 | Window Closed / Lapsed |