Enzyme-immobilization carriers and preparation thereof

Claim 1

An enzyme-immobilization carrier which comprises a macroporous, synthetic amphoteric ion-exchange resin having a specific surface area of at least 1 m 2 /g-resin containing macropores with a pore diameter of 100 A to 2,000 A, of which total volume is at least 0.1 cc/g-resin; said resin comprising (a) a phenol resin matrix having a primary amino group, secondary amino group, tertiary amino group, quaternary ammonium group or a mixture thereof as an anion-exchanger and a carboxymethyl group linked to the matrix through a phenolic ether linkage or to said primary or secondary amino group, wherein the cation═exchange capacity due to the carboxymethyl group is not less than 0.5 meq/g-resin and the anion-exchange capacity due to the amino group, substituted amino groups or the mixture thereof is not less than 1 meq/g-resin, or (b) a polystyrene or polyvinyl chloride matrix having at least a primary amino group, secondary amino group or a mixture thereof as an anion-exchanger and a carboxymethyl group linked to said primary or secondary amino group, wherein the cation-exchange capacity due to the carboxymethyl group is not less than 0.5 meq/g-resin and the anion-exchange capacity due to the amino group, substituted amino groups or the mixture thereof is not less than 1 meq/g-resin.