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US4238389A
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Valproate conjugation using dicarbonyls
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1979-02-12
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Granted: 1980-12-09
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Expires: 1999-02-12
Abstract
Abstract alpha -substituted derivatives of valproic acid are provided for conjugation to antigenic compositions, particularly poly(amino acids), and enzymes. The antigenic conjugates are employed for the production of antibodies, which find particular use in immunoassays for the determination of valproate, while the enzyme conjugate finds use in a homogeneous enzyme immunoassay for the determination of valproate. The compounds are synthesized by alkylating valproate at the tertiary carbon atom by an aliphatic chain with a terminal double bond which is cleaved to provide an acid or aldehyde group.
Inventors
- 👤 Danton K. Leung
- 👤 Prithipal Singh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 14:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9473; C; C07; C07C; C07C51/00; C07C51/16; C07C51/347; C07C51/353; C07C51/373; C07C51/54; C07C51/56; G01N33/53; G01N33/531; G01N33/532; G01N33/535; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S435/964; Y10S436/00; Y10S436/815; Y10S436/823
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
31 words
A conjugate of a compound selected from the group consisting of: 2-propyl-2-carboxymethylpentanoic acid, 2-propyl-2-(2'-carboxyethyl)pentanoic acid, 2,2-dipropylglutaric anhydride, 2,2-dipropylsuccinic anhydride, 6-carboxy-6-propylnonanal, and 2-carboxy-2-propylnonanoic acid, with a poly(amino acid) antigen or an enzyme.
Claim 2
Dependent Claim
31 words
A conjugate of a compound selected from the group consisting of: 2-propyl-2-carboxymethylpentanoic acid, 2-propyl-2-(2'-carboxyethyl)pentanoic acid, 2,2-dipropylglutaric anhydride, 2,2-dipropylsuccinic anhydride, 6-carboxy-6-propylnonanal, and 2-carboxy-2-propylnonanoic acid, with a poly(amino acid) antigen or an enzyme.
Claim 3
Dependent Claim
16 words
A conjugate according to claim 1 wherein said conjugate has an enzyme which is glucose-6-phosphate dehydrogenase.
Claim 4
Dependent Claim
10 words
Conjugate according to claim 1 having a poly(amino acid) antigen.
Claim 5
Dependent Claim
85 words
Compound of the formula: ##STR5## wherein: R is an aliphatic group of from 1 to 6 carbon atoms; W 1 is hydroxyl; when m is 0, W is a methylene bonded to Z through amino nitrogen, and Z is a poly(amino acid); when m is 1, W is a bond to Z, and Z is poly(amino acid) wherein W is bonded to said poly(amino acid) to amino groups to form amide linkages; and n is one to the molecular weight of Z divided by 500.
Claim 6
Dependent Claim
85 words
Compound of the formula: ##STR5## wherein: R is an aliphatic group of from 1 to 6 carbon atoms; W 1 is hydroxyl; when m is 0, W is a methylene bonded to Z through amino nitrogen, and Z is a poly(amino acid); when m is 1, W is a bond to Z, and Z is poly(amino acid) wherein W is bonded to said poly(amino acid) to amino groups to form amide linkages; and n is one to the molecular weight of Z divided by 500.
Claim 7
Dependent Claim
14 words
Compound according to claim 4, wherein R is from 1 to 4 carbon atoms.
Claim 8
Dependent Claim
18 words
Compound according to claim 5, wherein Z is an enzyme and n is of from 1 to 30.
Claim 9
Dependent Claim
22 words
Compound according to claim 5, wherein Z is an antigen and n is in the range of from about 1 to 500.
Claim 10
Dependent Claim
87 words
Compound of the formula: ##STR6## wherein: R is an alkylene group of from 1 to 6 carbon atoms; m 2 is 0 or 1; Z 2 is a poly(amino acid); n 2 is at least 1 and not greater than the molecular weight of Z 2 divided by 500; and when m 2 is 0, W 4 is methylene bonded to amino groups of Z 2 and when m 2 is 1, W 4 is a bond to amino groups of Z 2 to form amide linkages.
Claim 11
Dependent Claim
87 words
Compound of the formula: ##STR6## wherein: R is an alkylene group of from 1 to 6 carbon atoms; m 2 is 0 or 1; Z 2 is a poly(amino acid); n 2 is at least 1 and not greater than the molecular weight of Z 2 divided by 500; and when m 2 is 0, W 4 is methylene bonded to amino groups of Z 2 and when m 2 is 1, W 4 is a bond to amino groups of Z 2 to form amide linkages.
Claim 12
Dependent Claim
22 words
Compound according to claim 8, wherein Z 2 is an enzyme and n 2 is in the range of 2 to 16.
Claim 13
Dependent Claim
11 words
Compound according to claim 9, wherein said enzyme is glucose-6-phosphate dehydrogenase.
Claim 14
Dependent Claim
23 words
Compound according to claim 8, wherein Z 2 is an antigen, and n 2 is in the range of about 2 to 500.
Claim 15
Dependent Claim
11 words
Compound according to claim 11, wherein said antigen is a globulin.
Claim 16
Dependent Claim
11 words
Compound according to claim 11, wherein said antigen is an albumin.
Claim 17
Dependent Claim
10 words
Conjugate according to claim 3, wherein said antigen is globulin.
Claim 18
Dependent Claim
10 words
Conjugate according to claim 3, wherein said antigen is albumin.
References Cited (Backward Citations)
US3325361A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1983-12-09 | 1984-06-09 | 1984-12-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-12-09 | 1988-06-09 | 1988-12-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-12-09 | 1992-06-09 | 1992-12-09 | $7700.00 | Window Closed / Lapsed |