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US4237222A
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Lactate oxidase process for the manufacture thereof and analytical method and kit for the use of the same
Assignee: Asahi Kasei Corp
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Filing: 1979-06-18
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Granted: 1980-12-02
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Expires: 1999-06-18
Abstract
Abstract Novel enzyme lactate oxidase can be produced by culturing Pediococcus sp. B-0667, Streptococcus sp. B-0668, Aerococcus viridans IFO-12219 or Aerococcus viridans IFO-12317. It is useful for analysis for L-lactic acid, because it catalyzes the reaction of L-lactic acid and oxygen to form pyruvic acid and hydrogen peroxide. A kit containing the various reagents for such analysis is also provided by this invention.
Inventors
- 👤 Hideo Misaki
- 👤 Yoshifumi Horiuchi
- 👤 Kazuo Matsuura
- 👤 Saburo Harada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 18:46
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; C12Q; C12Q1/00; C12Q1/26
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
28 words
An enzyme lactate oxidase having at least the following substrate specificity and enzyme action: substrate specificity: L-lactic acid enzyme action: catalyzes the reaction L-lactic acid+O.sub.2 →pyruvic acid+H.sub.2 O.sub.2.
Claim 2
Dependent Claim
28 words
An enzyme lactate oxidase having at least the following substrate specificity and enzyme action: substrate specificity: L-lactic acid enzyme action: catalyzes the reaction L-lactic acid+O.sub.2 →pyruvic acid+H.sub.2 O.sub.2.
Claim 3
Dependent Claim
46 words
An enzyme lactate oxidase as claimed in claim 1 in which the said enzyme has an optimum pH of pH 6-7, an optimum temperature of about 35° C., an isoelectric point of pH 4.6±0.3 (measured by electrophoresis using carrier ampholite), and a molecular weight of 80,000±10,000.
Claim 4
Dependent Claim
67 words
A process for the manufacture of lactate oxidase having substrate specificity at least for L-lactic acid and catalyzing the reaction L-lactic acid+O.sub.2 →pyruvic acid+H.sub.2 O.sub.2 which comprises culturing a lactate oxidase-producing microorganism belonging to a genus selected from, Pediococcus sp. B-0667, Streptococcus sp. B-0668, Aerococcus viridans IFO-12219 and Aerococcus viridans IFO-12317, in a nutrient culture medium, and isolating the lactate oxidase thus produced from the cultured medium.
Claim 5
Dependent Claim
67 words
A process for the manufacture of lactate oxidase having substrate specificity at least for L-lactic acid and catalyzing the reaction L-lactic acid+O.sub.2 →pyruvic acid+H.sub.2 O.sub.2 which comprises culturing a lactate oxidase-producing microorganism belonging to a genus selected from, Pediococcus sp. B-0667, Streptococcus sp. B-0668, Aerococcus viridans IFO-12219 and Aerococcus viridans IFO-12317, in a nutrient culture medium, and isolating the lactate oxidase thus produced from the cultured medium.
Claim 6
Dependent Claim
45 words
A process as claimed in claim 3 in which the said lactate oxidase has an optimum pH of pH 6-7, an optimum temperature of about 35° C., an isoelectric point of pH 4.6±0.3 (measured by electrophoresis using carrier ampholite), and a molecular weight of 80,000±10,000.
Claim 7
Independent Claim
56 words
A method for the analysis of lactic acid in a sample containing lactic acid or a lactic acid-liberating system, comprising contacting said sample with lactate oxidase having substrate specificity at least for L-lactic acid and an enzyme action catalyzing the reaction L-lactic acid+O.sub.2 →pyruvic acid+H.sub.2 O.sub.2, and measuring generated hydrogen peroxide, pyruvic acid or consumed oxygen.
Claim 8
Independent Claim
56 words
A method for the analysis of lactic acid in a sample containing lactic acid or a lactic acid-liberating system, comprising contacting said sample with lactate oxidase having substrate specificity at least for L-lactic acid and an enzyme action catalyzing the reaction L-lactic acid+O.sub.2 →pyruvic acid+H.sub.2 O.sub.2, and measuring generated hydrogen peroxide, pyruvic acid or consumed oxygen.
Claim 9
Dependent Claim
44 words
A method as claimed in claim 5, in which said lactate oxidase has an optimum pH of ph 6-7, an optimum temperature of about 35° C., an isoelectric point of pH 4.6±0.3 (measured by electrophoresis using carrier ampholite), and a molecular weight of 80,000±10,000.
Claim 10
Dependent Claim
37 words
A kit for the analysis of lactic acid, which comprises a system containing at least lactate oxidase having substrate specificity at least for L-lactic acid and an enzyme action catalyzing the reaction L-lactic acid+O.sub.2 →pyruvic acid+H.sub.2 O.sub.2.
Claim 11
Dependent Claim
37 words
A kit for the analysis of lactic acid, which comprises a system containing at least lactate oxidase having substrate specificity at least for L-lactic acid and an enzyme action catalyzing the reaction L-lactic acid+O.sub.2 →pyruvic acid+H.sub.2 O.sub.2.
Claim 12
Dependent Claim
44 words
A kit as claimed in claim 7, in which said lactate oxidase has an optimum pH of pH 6-7, an optimum temperature of about 35° C., an isoelectric point of pH 4.6±0.3 (measured by electrophoresis using carrier ampholite), and a molecular weight of 80,000±10,000.
Claim 13
Dependent Claim
21 words
A kit as claimed in claim 7, comprising at least a combination of lactate oxidase and an indicator for hydrogen peroxide.
Claim 14
Dependent Claim
21 words
A kit as claimed in claim 9, in which said indicator for hydrogen peroxide comprises at least 4-aminoantipyrin, N,N-dimethylaniline and peroxides.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-12-02 | 1984-06-02 | 1984-12-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-12-02 | 1988-06-02 | 1988-12-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-12-02 | 1992-06-02 | 1992-12-02 | $7700.00 | Window Closed / Lapsed |