US4237218A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Micro-carrier cell culture
Assignee: Bio Rad Laboratories Inc
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Filing: 1979-02-09
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Granted: 1980-12-02
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Expires: 1999-02-09
Abstract
Abstract Insoluble cationic acrylamide copolymers for use as cell growth carriers and a novel copolymer of dimethylaminopropylmethacrylamide, acrylamide and methylene-bis-acrylamide useful as both a carrier for cell growth and as a medium for ion exchange chromatography of aqueous protein solutions.
Inventors
- 👤 James F. Monthony
- 👤 Norman D. Schwartz
- 👤 Donald F. Hollis
- 👤 Gian D. Polastri
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:29
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N5/0075; C08; C08F; C08F220/00; C08F220/02; C08F220/52; C08F220/54; C08F220/60; C12N2533/00; C12N2533/30
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
90 words
A method for growing attachment-dependent cells comprising the steps of providing a suspension comprising cell carriers, an inoculum of said cells and nutrient-containing growth medium, and agitating sufficiently to keep said suspension in motion at a temperature between 20° C. and 45° C., said cell carriers comprising an insoluble cationic copolymer having a charge density of 0.050-0.150 meq/ml formed from the copolymerization of a) a hydrophilic monomer; b) a cross-linking monomer selected from the group consisting of di- and poly vinyls; and c) a cationic monomer of the formula: ##STR4##
Claim 2
Independent Claim
90 words
A method for growing attachment-dependent cells comprising the steps of providing a suspension comprising cell carriers, an inoculum of said cells and nutrient-containing growth medium, and agitating sufficiently to keep said suspension in motion at a temperature between 20° C. and 45° C., said cell carriers comprising an insoluble cationic copolymer having a charge density of 0.050-0.150 meq/ml formed from the copolymerization of a) a hydrophilic monomer; b) a cross-linking monomer selected from the group consisting of di- and poly vinyls; and c) a cationic monomer of the formula: ##STR4##
Claim 3
Dependent Claim
38 words
A method according to claim 1 wherein said hydrophilic monomer is of the formula: ##STR5## wherein R 1 is H or lower alkyl, and ##STR6## wherein X is lower alkoxy, lower alkyl amine, hydroxyalkylamine or NH 2 .
Claim 4
Dependent Claim
25 words
A method according to claim 1 wherein said crosslinking monomer is selected from the group consisting of alkylenediacrylates, alkylenedimethacrylates, oligo and polyglycoldiacrylates, alkylene-bis-acrylamides and alkylene-bis-methacrylamides.
Claim 5
Dependent Claim
29 words
A method according to claim 1 wherein said hydrophilic monomer comprises 30-38 weight percent, said cationic monomer comprises 60-75 weight percent and said crosslinking polymer comprises 7-9 weight percent.
Claim 6
Dependent Claim
23 words
A method according to claim 1 wherein said hydrophilic monomer is acrylamide, said crosslinking monomer is methylene-bis-acrylamide and said cationic monomer is dimethylaminopropylmethacrylamide.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-12-02 | 1984-06-02 | 1984-12-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-12-02 | 1988-06-02 | 1988-12-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-12-02 | 1992-06-02 | 1992-12-02 | $7700.00 | Window Closed / Lapsed |