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US4235970A
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Protease inactivated α-amylase preparations
Assignee: Genencor International Wisconsin Inc
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Filing: 1976-08-05
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Granted: 1980-11-25
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Expires: 1996-08-05
Abstract
Abstract Protease enzyme impurities contained in bacterial alpha -amylase enzyme preparations are inactivated by a mild heat treatment in the presence of a protective material. Useful protective materials include calcium and starch hydrolysates such as corn syrup. The protease-free alpha -amylase can then be used to solubilize starch materials by various granular starch and conventional processes. Hydrolysates obtained contain significantly less soluble protein than those prepared using untreated alpha -amylases. A preferred alpha -amylase enzyme preparation is one derived from a Bacillus licheniformis microorganism.
Inventors
- 👤 Harry W. Leach
- 👤 Ronald Hebeda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 23:50
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2408; C12N9/2411; C12N9/2414; C12N9/2417; Y; Y10; Y10S; Y10S435/00; Y10S435/813; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/836
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
94 words
A process for the selective inactivation of proteolytic enzyme impurities in a bacterial α-amylase enzyme preparation derived from Bacillus licheniformis comprising the steps of: (a) introducing into the preparation sufficient water soluble calcium salt to yield a total calcium ion concentration of from about 0.5% to about 1.5%, dry basis; (b) heating the resulting mixture to a temperature in the range of from about 70° C. to about 90° C.; and (c) maintaining the temperature for a period of time sufficient to substantially completely inactivate the proteolytic enzyme without substantially reducing the α-amylase activity.
Claim 2
Dependent Claim
94 words
A process for the selective inactivation of proteolytic enzyme impurities in a bacterial α-amylase enzyme preparation derived from Bacillus licheniformis comprising the steps of: (a) introducing into the preparation sufficient water soluble calcium salt to yield a total calcium ion concentration of from about 0.5% to about 1.5%, dry basis; (b) heating the resulting mixture to a temperature in the range of from about 70° C. to about 90° C.; and (c) maintaining the temperature for a period of time sufficient to substantially completely inactivate the proteolytic enzyme without substantially reducing the α-amylase activity.
Claim 3
Dependent Claim
24 words
A process in accordance with claim 1, wherein the pH of the preparation is in the range of from about 5 to about 8.
Claim 4
Dependent Claim
24 words
A process in accordance with claim 1, wherein the temperature is maintained for a period of from about 30 minutes to about 3 hours.
Claim 5
Dependent Claim
23 words
A process in accordance with claim 1, wherein at least a portion of said calcium ion was originally present in said enzyme preparation.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-11-25 | 1984-05-26 | 1984-11-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-11-25 | 1988-05-26 | 1988-11-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-11-25 | 1992-05-26 | 1992-11-25 | $7700.00 | Window Closed / Lapsed |