Patent US4235964A successfully refreshed from Google Patents.
US4235964A
Expired / Lapsed
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Method for testing and identifying microorganisms
Assignee: Individual
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Filing: 1978-09-28
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Granted: 1980-11-25
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Expires: 1998-09-28
Abstract
Abstract In accordance with the invention there is provided a method for identifying or testing an anaerobic microorganism wherein an aqueous suspension of a culture of the microorganism having a culture density of about 2×10 8 cells/ml, is brought into contact with an aqueous solution containing an oxidation-reduction indicator which substantially irreversibly undergoes a change in color upon being reduced and a biodegradable test substrate compound which, when catabolized by a microorganism, will engender reduction of the indicator, said aqueous suspension containing nutrient in a concentration sufficient to support microorganism culture growth without engendering reduction of the indicator when said suspension contacts said solution, and said suspension also containing a compound which reduces O 2 but does not reduce the indicator.
Inventors
- 👤 Barry R. Bochner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 21:56
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12M; C12M41/00; C12M41/30; C12M41/36; C12Q2304/00; C12Q2304/20; C12Q2304/24; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/21; G01N2333/24; G01N2333/255
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
162 words
A method for identifying or testing a microorganism comprising preparing a suspension of a culture of the microorganism in a nutrient solution to a culture density of about 2×10 8 cells/ml, and then bringing said suspension into contact with an aqueous solution containing an oxidation-reduction indicator which substantially irreversibly undergoes a change in color upon being reduced and a biodegradable test substrate compound which, when catabolized by a microorganism, will engender reduction of the indicator, the concentration of nutrient in said suspension being insufficient to engender reduction of the indicator when said suspension contacts said solution, said suspension containing a compound which reduces O 2 but which does not reduce the oxidation-reduction indicator and which is present in said suspension in an amount sufficient to reduce all the O 2 therein, at least one of said suspension and said solution containing a buffer to maintain the pH at from about 4.5 to 8 while said suspension is in contact with said solution.
Claim 2
Independent Claim
162 words
A method for identifying or testing a microorganism comprising preparing a suspension of a culture of the microorganism in a nutrient solution to a culture density of about 2×10 8 cells/ml, and then bringing said suspension into contact with an aqueous solution containing an oxidation-reduction indicator which substantially irreversibly undergoes a change in color upon being reduced and a biodegradable test substrate compound which, when catabolized by a microorganism, will engender reduction of the indicator, the concentration of nutrient in said suspension being insufficient to engender reduction of the indicator when said suspension contacts said solution, said suspension containing a compound which reduces O 2 but which does not reduce the oxidation-reduction indicator and which is present in said suspension in an amount sufficient to reduce all the O 2 therein, at least one of said suspension and said solution containing a buffer to maintain the pH at from about 4.5 to 8 while said suspension is in contact with said solution.
Claim 3
Independent Claim
192 words
A method for identifying or testing a microorganism comprising preparing a suspension of a culture of the microorganism in a nutrient solution to a culture density of at least 2×10 8 cells/ml, said suspension having a pH from about 4.5 to 8 and being substantially sealed from the ambient atmosphere, and then unsealing and bringing said suspension into contact with a solution which is exposed to oxygen from the ambient atmosphere, which has a pH of from about 4.5 to 8, and which contains an oxidation-reduction indicator which substantially irreversibly undergoes a change in color upon being reduced and a biodegradable test substrate compound which, when catabolized by a microorganism, will engender reduction of the indicator, the concentration of the nutrient in said suspension being insufficient to engender reduction of the indicator when said suspension contacts said solution, said suspension containing a compound which reduces O 2 but which does not reduce the oxidation-reduction indicator at a pH of from 4.5 to 8 in the presence of O 2 , said compound which reduces O 2 being present in said suspension in an amount sufficient to reduce all the O 2 therein.
Claim 4
Independent Claim
192 words
A method for identifying or testing a microorganism comprising preparing a suspension of a culture of the microorganism in a nutrient solution to a culture density of at least 2×10 8 cells/ml, said suspension having a pH from about 4.5 to 8 and being substantially sealed from the ambient atmosphere, and then unsealing and bringing said suspension into contact with a solution which is exposed to oxygen from the ambient atmosphere, which has a pH of from about 4.5 to 8, and which contains an oxidation-reduction indicator which substantially irreversibly undergoes a change in color upon being reduced and a biodegradable test substrate compound which, when catabolized by a microorganism, will engender reduction of the indicator, the concentration of the nutrient in said suspension being insufficient to engender reduction of the indicator when said suspension contacts said solution, said suspension containing a compound which reduces O 2 but which does not reduce the oxidation-reduction indicator at a pH of from 4.5 to 8 in the presence of O 2 , said compound which reduces O 2 being present in said suspension in an amount sufficient to reduce all the O 2 therein.
Claim 5
Dependent Claim
43 words
A method as set forth in claim 2 wherein said oxidation-reduction indicator is triphenyl tetrazolium chloride and wherein said compound which reduces O 2 is glutathione and is present in said suspension in an amount of at least about 5 millimoles per liter.
References Cited (Backward Citations)
US4129483A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-11-25 | 1984-05-26 | 1984-11-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-11-25 | 1988-05-26 | 1988-11-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-11-25 | 1992-05-26 | 1992-11-25 | $7700.00 | Window Closed / Lapsed |