US4235864A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Simultaneous radio immunoassay of multiple antigens and _assay for cocaine metabolites
Assignee: Research Corp
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Filing: 1978-04-10
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Granted: 1980-11-25
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Expires: 1998-04-10
Abstract
Abstract This invention includes an antigenic conjugate of ecgonine and a carrier which elicits anti-benzoylecgonine (cocaine metabolite) serum useful for the detection of cocaine metabolites in human organs and body fluids by immunoassay. The invention also includes the method of producing the anti-benzoylecgonine serum, the immunoassay method and composition including the anti-benzoylecgonine serum and a labeled benzoylecgonine and a non-labeled benzoylecgonine. The invention also includes a composition comprising a plurality of antigenic conjugates which elicit a multivalent anti-serum capable of complexing a plurality of antigens in a multiple immunoassay detection method. The invention includes the method of producing the multivalent anti-serum and an immunoassay method employing the multivalent anti-serum. The invention further includes a composition comprising the multivalent anti-serum and a plurality of labeled antigens and non-labeled antigens.
Inventors
- 👤 Balkrishena Kaul
- 👤 Bernard Davidow
- 👤 Stephen J. Millian
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 04:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/946; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/816; Y10S436/823
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
81 words
In an immunoassay method for the detection of a cocaine metabolite in a biological tissue or fluid wherein an anti-serum is combined with said biological tissue or fluid to form a complex with said cocaine metabolite, said complex being detectable in an assay, the improvement comprising employing an anti-benzoylecgonine serum produced by immunizing an animal by injecting into said animal an antigenic conjugate of ecgonine or the acetate thereof and a peptide, protein or polysaccharide and eliciting serum from said animal.
Claim 2
Dependent Claim
81 words
In an immunoassay method for the detection of a cocaine metabolite in a biological tissue or fluid wherein an anti-serum is combined with said biological tissue or fluid to form a complex with said cocaine metabolite, said complex being detectable in an assay, the improvement comprising employing an anti-benzoylecgonine serum produced by immunizing an animal by injecting into said animal an antigenic conjugate of ecgonine or the acetate thereof and a peptide, protein or polysaccharide and eliciting serum from said animal.
Claim 3
Dependent Claim
17 words
The immunoassay method of claim 1 wherein said conjugate is a conjugate of ecgonine and sheep gamma-globulin.
Claim 4
Dependent Claim
64 words
A composition suitable for use in an immunoassay method for detecting a cocaine metabolite in a biological tissue or fluid comprising an antibenzoylecgonine serum and a labeled benzoylecgonine, wherein said anti-benzoylecgonine serum is produced by immunizing an animal by injecting into said animal an antigenic conjugate of ecgonine or the acetate thereof and a peptide, protein or polysaccharide and eliciting serum from said animal.
Claim 5
Dependent Claim
64 words
A composition suitable for use in an immunoassay method for detecting a cocaine metabolite in a biological tissue or fluid comprising an antibenzoylecgonine serum and a labeled benzoylecgonine, wherein said anti-benzoylecgonine serum is produced by immunizing an animal by injecting into said animal an antigenic conjugate of ecgonine or the acetate thereof and a peptide, protein or polysaccharide and eliciting serum from said animal.
Claim 6
Dependent Claim
14 words
The composition of claim 3 wherein said labeled benzoylecgonine is I 125 -benzoylecgonine derivative.
Claim 7
Dependent Claim
27 words
The composition of claim 4 wherein said anti-serum is produced employing an antigenic conjugate of ecgonine or the acetate thereof and a gamma globulin or a thyroglobulin.
Claim 8
Dependent Claim
20 words
The composition of claim 5 wherein said anti-serum is produced employing an antigenic conjugate of ecgonine and sheep gamma globulin.
Claim 9
Dependent Claim
43 words
A composition comprising anti-benzoylecgonine serum and benzoylecgonine wherein said anti-benzoylecgonine serum is produced by immunizing an animal by injecting into said animal an antigenic conjugate of ecgonine or the acetate thereof and a peptide protein or polysaccharide and eliciting serum from said animal.
Claim 10
Dependent Claim
43 words
A composition comprising anti-benzoylecgonine serum and benzoylecgonine wherein said anti-benzoylecgonine serum is produced by immunizing an animal by injecting into said animal an antigenic conjugate of ecgonine or the acetate thereof and a peptide protein or polysaccharide and eliciting serum from said animal.
Claim 11
Dependent Claim
27 words
The composition of claim 7 wherein said anti-serum is produced employing an antigenic conjugate of ecgonine or an acetate thereof and a gamma globulin or a thyroglobulin.
Claim 12
Dependent Claim
20 words
The composition of claim 7 wherein said anti-serum is produced employing an antigenic conjugate of ecgonine and sheep gamma globulin.
References Cited (Backward Citations)
US3966764A
Cited
US4045420A
Cited
US3917582A
Cited
US4041040A
Cited
US4102979A
Cited
US4071522A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-11-25 | 1984-05-26 | 1984-11-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-11-25 | 1988-05-26 | 1988-11-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-11-25 | 1992-05-26 | 1992-11-25 | $7700.00 | Window Closed / Lapsed |