US4102979A
Expired / Lapsed
US
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Radioimmunoassay for benzoylecgonine
Assignee: Hoffmann La Roche Inc
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Filing: 1976-09-22
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Granted: 1978-07-25
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Expires: 1996-09-22
Abstract
Abstract A radioimmunoassay for cocaine and the cocaine metabolite benzoylecgonine is described. The assay utilizes a novel labelled compound 125I-4-hydroxybenzoylecgonine.
Inventors
- 👤 James Gordon Christenson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 04:06
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/946; C; C07; C07D; C07D451/00; C07D451/02; C07D451/04; C07D451/06; C07D451/12; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/816
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
127 words
A method for the radioimmunoassay of benzoylecgonine and cocaine in a sample, which method comprises mixing said sample with a known amount of a radiolabeled benzoylecgonine compound selected from the group consisting of benzoylecgonine - 3 H having a specific activity suitable for radioimmunoassay procedures and 125 I-4-hydroxybenzoylecgonine thereof and an antibody which will selectively complex with said benzoylecgonine or cocaine, measuring the degree of binding of said radio-labeled benzoylecgonine compound, and determining the amount of benzoylecgonine and cocaine present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of benzoylecgonine or cocaine with fixed amounts of labeled benzoylecgonine compound and said antibody and determining the degree of binding for each known amount of said benzoylecgonine or cocaine.
Claim 2
Independent Claim
127 words
A method for the radioimmunoassay of benzoylecgonine and cocaine in a sample, which method comprises mixing said sample with a known amount of a radiolabeled benzoylecgonine compound selected from the group consisting of benzoylecgonine - 3 H having a specific activity suitable for radioimmunoassay procedures and 125 I-4-hydroxybenzoylecgonine thereof and an antibody which will selectively complex with said benzoylecgonine or cocaine, measuring the degree of binding of said radio-labeled benzoylecgonine compound, and determining the amount of benzoylecgonine and cocaine present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of benzoylecgonine or cocaine with fixed amounts of labeled benzoylecgonine compound and said antibody and determining the degree of binding for each known amount of said benzoylecgonine or cocaine.
Claim 3
Dependent Claim
11 words
The method of claim 1 wherein radiolabeled benzoylecgonine compound is 4-hydroxybenzoylecgonine.
Claim 4
Dependent Claim
22 words
The method of claim 1 wherein the radiolabelled benzoylecgonine compound is benzoylecgonine- 3 H having a specific activity suitable for radioimmunoassay procedures.
Claim 5
Dependent Claim
11 words
The method of claim 1 wherein said radioimmunoassay is for benzoylecgonine.
Claim 6
Dependent Claim
2 words
125 I-4-hydroxybenzoylecgonine.
Claim 7
Dependent Claim
2 words
125 I-4-hydroxybenzoylecgonine.
References Cited (Backward Citations)
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US3498989A
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US3966744A
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US3709868A
Cited
US3888866A
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US3917582A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-07-24 | 1982-01-23 | 1982-07-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-07-24 | 1986-01-23 | 1986-07-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-07-24 | 1990-01-23 | 1990-07-25 | $7700.00 | Window Closed / Lapsed |