Patent US4234680A successfully refreshed from Google Patents.
US4234680A
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Method for terminating a peroxidase catalyzed reaction
Assignee: CNA Holdings LLC
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Filing: 1979-08-03
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Granted: 1980-11-18
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Expires: 1999-08-03
Abstract
Abstract Addition of a low concentration of an alkali metal metabisulfite completely stops color development in a peroxidase-labeled immunoassay wherein the peroxidase catalyzed reaction comprises oxidation of an achromatic substrate to the corresponding chromatic product in the presence of a peroxide.
Inventors
- 👤 Richard C. Hevey
- 👤 Mark K. Malmros
- 👤 Wayne W. Petko
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 20:40
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; C; C12; C12N; C12N9/00; C12N9/99; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; Y; Y10; Y10S; Y10S435/00; Y10S435/963
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
88 words
A method for terminating a peroxidase catalyzed reaction employed in a peroxidase-labeled enzyme immunoassay for detecting and quantitating ligands, said peroxidase catalyzed reaction comprising the oxidation of a substrate selected from the group consisting of o-phenylenediamine, m-phenylenediamine, dianisidine, aniline, p-aminobenzoic acid and m-aminosalicylic acid in the presence of a peroxide selected from the group consisting of hydrogen peroxide, methyl peroxide and ethyl peroxide, which method comprises adding to the enzyme immunoassay subsequent to the peroxidase catalyzed reaction an amount of alkali metal metabisulfite sufficient to terminate said reaction.
Claim 2
Independent Claim
88 words
A method for terminating a peroxidase catalyzed reaction employed in a peroxidase-labeled enzyme immunoassay for detecting and quantitating ligands, said peroxidase catalyzed reaction comprising the oxidation of a substrate selected from the group consisting of o-phenylenediamine, m-phenylenediamine, dianisidine, aniline, p-aminobenzoic acid and m-aminosalicylic acid in the presence of a peroxide selected from the group consisting of hydrogen peroxide, methyl peroxide and ethyl peroxide, which method comprises adding to the enzyme immunoassay subsequent to the peroxidase catalyzed reaction an amount of alkali metal metabisulfite sufficient to terminate said reaction.
Claim 3
Dependent Claim
15 words
A method according to claim 1 wherein said peroxidase-labeled enzyme immunoassay is a heterogeneous assay.
Claim 4
Dependent Claim
33 words
A method according to claim 2 wherein said peroxidase catalyzed reaction comprises oxidation of a substrate selected from the group consisting of o-phenylenediamine, m-aminosalicylic acid and dianisidine in the presence of hydrogen peroxide.
Claim 5
Dependent Claim
12 words
A method according to claim 3 wherein said peroxidase is horseradish peroxidase.
Claim 6
Dependent Claim
14 words
A method according to claim 4 wherein the alkali metal metabisulfite is sodium metabisulfite.
Claim 7
Dependent Claim
20 words
A method according to claim 5 wherein said heterogeneous assay is a sandwich assay for detecting and quantitating human IgE.
Claim 8
Dependent Claim
25 words
A method according to claim 1 wherein the amount of alkali metabisulfite employed is sufficient to provide a concentration of from 0.091 to 0.182 M.
References Cited (Backward Citations)
US4169012A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-11-18 | 1984-05-19 | 1984-11-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-11-18 | 1988-05-19 | 1988-11-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-11-18 | 1992-05-19 | 1992-11-18 | $7700.00 | Window Closed / Lapsed |