Abstract A fermentation process for the production of microbial biomass and a heteropolysaccharide biopolymer using methane as a carbon source. The biomass is suitable for use as a feedstuff and the polymer can be used as a thickening agent e.g. in foods or in the oil industry for use in drilling muds and to assist the recovery of subterranean deposits of oil. The process comprises cultivating at least one strain of a methane utilizing and extracellular heteropolysaccharide biopolymer forming bacterium of the group Methylomonas in a broth comprising an aqueous nutrient medium and a utilizable nitrogen source in the presence of a gas containing methane as a carbon source and a gas containing free oxygen and recovering biomass and heteropolysaccharide biopolymer from the cultivated broth.
A polyanionic heteropolysaccharide biopolymer having molecular weight in the range of 5.0×10 5 to 2.0×10 8 when measured by gel permeation chromatography and comprising the saccharide residues glucose, fucose, mannose, galactose and uronic acid in the following proportions based on weight of total saccharide content: from 38 to 48% glucose; from 11 to 20% fucose; from 7 to 21% mannose; from 11 to 18% galactose and from 10 to 20% uronic acid.
Claim 2
Dependent Claim
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A polyanionic heteropolysaccharide biopolymer having molecular weight in the range of 5.0×10 5 to 2.0×10 8 when measured by gel permeation chromatography and comprising the saccharide residues glucose, fucose, mannose, galactose and uronic acid in the following proportions based on weight of total saccharide content: from 38 to 48% glucose; from 11 to 20% fucose; from 7 to 21% mannose; from 11 to 18% galactose and from 10 to 20% uronic acid.