Patent US4228154A successfully refreshed from Google Patents.
US4228154A
Expired / Lapsed
US
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Purification of plasma albumin by ion exchange chromatography
Assignee: Armour Pharmaceutical Co
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Filing: 1979-02-26
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Granted: 1980-10-14
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Expires: 1999-02-26
Abstract
Abstract Purified plasma albumin is obtained by ion exchange chromatographic procedures and in the absence of process steps involving precipitation or desorption of the albumin.
Inventors
- 👤 Joseph D. Fisher
- 👤 Willie M. Curry
- 👤 Michael E. Hrinda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 22:17
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/76; C07K14/765; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/831
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
162 words
In the process for isolation of purified plasma albumin wherein plasma is processed by cold alcohol fractionation to develop an albumin-containing Cohn II+III supernatant fluid, an improvement in the procedure for further isolation of albumin from proteinaceous material in the fluid, said improvement comprising the steps of: (a) intimately contacting said fluid with lipoprotein extractant means; (b) adjusting the pH of the fluid to from about 4.5 to 4.9 and intimately contacting the pH-adjusted fluid with an anionic ion exchanger means for removing from said ph-adjusted fluid proteinaceous materials having an isoelectric point below that of albumin; and (c) adjusting the pH of said to fluid from about 5.1 to 5.5 and intimately contacting the pH-adjusted fluid with a cationic ion exchanger means for removing from said pH-adjusted fluid proteinaceous materials having an isoelectric point above that of albumin, whereby an albumin-containing fluid product is obtained substantially free of contaminating proteinaceous materials having an isoelectric point below or above that of albumin.
Claim 2
Dependent Claim
162 words
In the process for isolation of purified plasma albumin wherein plasma is processed by cold alcohol fractionation to develop an albumin-containing Cohn II+III supernatant fluid, an improvement in the procedure for further isolation of albumin from proteinaceous material in the fluid, said improvement comprising the steps of: (a) intimately contacting said fluid with lipoprotein extractant means; (b) adjusting the pH of the fluid to from about 4.5 to 4.9 and intimately contacting the pH-adjusted fluid with an anionic ion exchanger means for removing from said ph-adjusted fluid proteinaceous materials having an isoelectric point below that of albumin; and (c) adjusting the pH of said to fluid from about 5.1 to 5.5 and intimately contacting the pH-adjusted fluid with a cationic ion exchanger means for removing from said pH-adjusted fluid proteinaceous materials having an isoelectric point above that of albumin, whereby an albumin-containing fluid product is obtained substantially free of contaminating proteinaceous materials having an isoelectric point below or above that of albumin.
Claim 3
Dependent Claim
24 words
The improvement of claim 1 wherein said steps are performed in consecutive sequence, beginning with step (a), followed by (b), and followed by (c).
Claim 4
Dependent Claim
15 words
The improvement of claim 1 wherein said lipoprotein extractant means comprises finely divided fume silica.
Claim 5
Dependent Claim
23 words
The improvement of claim 1 wherein said anionic ion exchanger means comprises a modified dextran to which quarternary amine functional groups are attached.
Claim 6
Dependent Claim
23 words
The improvement of claim 1 wherein said cationic ion exchanger means comprises a modified dextran to which sulfonic acid functional groups are attached.
Claim 7
Dependent Claim
15 words
The improvement of claim 1 further including the step of removing alcohol from said fluid.
Claim 8
Dependent Claim
21 words
The improvement of claim 6 wherein said alcohol removal step precedes the operation of any of steps (a), (b), or (c).
Claim 9
Dependent Claim
17 words
The improvement of claim 1 wherein said pH adjustment in step (b) comprises adjustment to pH 4.7.
Claim 10
Dependent Claim
17 words
The improvement of claim 1 wherein said pH adjustment in step (c) comprises adjustment to pH 5.3.
Claim 11
Dependent Claim
20 words
The improvement of claim 1 wherein step (a) is carried out over a period of from 5 to 64 hours.
Claim 12
Dependent Claim
24 words
The improvement of claim 1 further including the step of concentrating said fluid product to form a therapeutic albumin solution of desired albumin concentration.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-10-14 | 1984-04-14 | 1984-10-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-10-14 | 1988-04-14 | 1988-10-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-10-14 | 1992-04-14 | 1992-10-14 | $7700.00 | Window Closed / Lapsed |