US4222934A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Preparation of albumin using ethanol
Assignee: American Red Cross
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Filing: 1979-04-12
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Granted: 1980-09-16
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Expires: 1999-04-12
Abstract
Abstract Methods for the large scale preparation of clinical albumin are disclosed. One method includes dilution of plasma with a NaCl solution containing disodium ethylene dinitrilo tetraacetate and a stabilizer such as sodium caprylate. The resulting solution is then subjected to heating and cooling steps, followed by the addition of polyethylene glycol to precipitate impurities, with albumin remaining in the supernatant. Isoelectric precipitation is then employed to recover the desired albumin product, which is devoid of the albumin dimer. In an alternative method, ethanol is employed as the precipitating agent rather than polyethylene glycol.
Inventors
- 👤 Yu L. Hao
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 00:36
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/76; C07K14/765; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
103 words
A method for the preparation of albumin comprising: (a) adjusting the pH of plasma in liquid form to about 6.7; (b) heating the plasma at a temperature of approximately 60° C. for about 11/2 hours; (c) adjusting the pH of the plasma to about 5.7; (d) precipitating impurities from the plasma by the addition of ethanol in an amount sufficient to give a final concentration of about 40 to 44% in the plasma along with cooling of the plasma to about -5° C., with the albumin remaining in the supernatant; and (e) precipitating albumin from said supernatant at a pH of about 4.8.
Claim 2
Independent Claim
103 words
A method for the preparation of albumin comprising: (a) adjusting the pH of plasma in liquid form to about 6.7; (b) heating the plasma at a temperature of approximately 60° C. for about 11/2 hours; (c) adjusting the pH of the plasma to about 5.7; (d) precipitating impurities from the plasma by the addition of ethanol in an amount sufficient to give a final concentration of about 40 to 44% in the plasma along with cooling of the plasma to about -5° C., with the albumin remaining in the supernatant; and (e) precipitating albumin from said supernatant at a pH of about 4.8.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-09-16 | 1984-03-17 | 1984-09-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-09-16 | 1988-03-17 | 1988-09-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-09-16 | 1992-03-17 | 1992-09-16 | $7700.00 | Window Closed / Lapsed |