Patent US4222836A successfully refreshed from Google Patents.
US4222836A
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Acidic agar gel electrochromatography of glycohemoglobins
Assignee: Bayer Corp
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Filing: 1979-06-01
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Granted: 1980-09-16
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Expires: 1999-06-01
Abstract
Abstract Glycosylated hemoglobins are separated from other hemoglobin variants by electrochromatography on an agar gel at an acidic pH which utilizes: A. an agar gel having a wet thickness of from about 0.1 to about 0.5 mm., B. a citrate gel buffer having a citrate concentration of from about 0.02 to about 0.05 M and a pH of from about 5.8 to about 6.8, C. a citrate well buffer having a citrate concentration of from about 0.05 to about 0.1 M and a pH from about 6.0 to about 6.5, and D. a potential of from about 25 to about 100 volts.
Inventors
- 👤 Robert J. Kerr
- 👤 Milos Stastny
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 19:26
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
122 words
In a method for discretely separating glycohemoglobins from nonglycosylated hemoglobins by citrate agar gel electrochromatography in which the gel buffer has a citrate concentration of 0.05 M and a pH of 6.2, the improvement which comprises employing: A. an agar gel having a wet thickness of from about 0.1 to about 0.5 mm., B. a citrate gel buffer having a citrate concentration of from about 0.02 to about 0.05 M and a pH of from about 5.8 to about 6.8, C. a citrate well buffer having a citrate concentration of from about 0.05 to about 0.1 M and a pH of from about 6.0 to about 6.5, and D. a field strength of from about 5 to about 20 volts per cm.
Claim 2
Dependent Claim
122 words
In a method for discretely separating glycohemoglobins from nonglycosylated hemoglobins by citrate agar gel electrochromatography in which the gel buffer has a citrate concentration of 0.05 M and a pH of 6.2, the improvement which comprises employing: A. an agar gel having a wet thickness of from about 0.1 to about 0.5 mm., B. a citrate gel buffer having a citrate concentration of from about 0.02 to about 0.05 M and a pH of from about 5.8 to about 6.8, C. a citrate well buffer having a citrate concentration of from about 0.05 to about 0.1 M and a pH of from about 6.0 to about 6.5, and D. a field strength of from about 5 to about 20 volts per cm.
Claim 3
Dependent Claim
19 words
The method of claim 1 in which the gel wet thickness is from about 0.3 to about 0.4 mm.
Claim 4
Dependent Claim
18 words
The method of claim 1 in which the gel buffer has a citrate concentration of about 0.038 M.
Claim 5
Dependent Claim
20 words
The method of claim 1 in which the gel buffer has a pH of from about 6.0 to about 6.3.
Claim 6
Dependent Claim
18 words
The method of claim 1 in which the well buffer has a citrate concentration of about 0.1 M.
Claim 7
Dependent Claim
20 words
The method of claim 1 in which the well buffer has a pH of from about 6.0 to about 6.3.
Claim 8
Dependent Claim
16 words
The method of claim 6 in which the well buffer has a pH of about 6.0.
Claim 9
Dependent Claim
16 words
The method of claim 1 in which the field strength is about 10 volts per cm.
Claim 10
Dependent Claim
24 words
The method of claim 1 in which the gel contains a humectant at a level of from 0 to about 10% weight per volume.
Claim 11
Dependent Claim
11 words
The method of claim 9 in which the humectant is sorbitol.
Claim 12
Dependent Claim
20 words
The method of claim 1 in which the sorbitol is present at a level of about 4% weight per volume.
Claim 13
Dependent Claim
11 words
The method of claim 1 in which the humectant is glycerol.
Claim 14
Dependent Claim
20 words
The method of claim 1 in which the glycerol is present at a level of about 1.3% weight per volume.
Claim 15
Dependent Claim
16 words
The method of claim 1 in which the humectant is a mixture of sorbitol and glycerol.
Claim 16
Dependent Claim
28 words
The method of claim 14 in which the sorbitol and glycerol are present at levels of about 4% weight per volume and about 1.3% weight per volume, respectively.
Claim 17
Dependent Claim
25 words
The method of claim 1 in which the gel contains a wetting agent at a level of from 0 to about 0.1% weight per volume.
Claim 18
Dependent Claim
13 words
The method of claim 16 in which the wetting agent is polyvinyl alcohol.
Claim 19
Dependent Claim
21 words
The method of claim 17 in which the polyvinyl alcohol is present at a level of about 0.017% weight per volume.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-09-16 | 1984-03-17 | 1984-09-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-09-16 | 1988-03-17 | 1988-09-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-09-16 | 1992-03-17 | 1992-09-16 | $7700.00 | Window Closed / Lapsed |