Patent US4220565A successfully refreshed from Google Patents.
US4220565A
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Immunochemical conjugates: method and composition
Assignee: Scripps Research Institute
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Filing: 1979-01-18
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Granted: 1980-09-02
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Expires: 1999-01-18
Abstract
Abstract Techniques for preparing stable conjugates of protein-D-GL which can then be isolated in pure form are disclosed, using hen egg ovalbumin (OVA) as a prototype protein, and conjugation and purification methods are described which involve (1) application of a coupling method employing m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) as the coupling reagent, and (2) introduction of biotin moieties into D-GL molecules to allow application of avidinbiotin system for affinity chromatographic purification of conjugates. These methods are applicable to the preparation of D-GL conjugates of insulin and Ragweed Antigen E and are of general applicability.
Inventors
- 👤 David H. Katz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 16:03
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/10; A; A61; A61K; A61K39/00; A61K39/35; A61K39/36; A61K47/00; A61K47/50; A61K47/51; A61K47/62; A61K47/64; A61K47/645; C07K14/00; C07K14/435; C07K14/575; C07K14/62; A61K2039/60; A61K2039/6093; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
74 words
An improved method of preparing immunosuppressive conjugates of D-glutamic acid-D-lysine copolymer and an antigen, comprising the steps of: (a) reacting D-glutamic acid-D-lysine copolymer with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-D-GL or with S-acetylmercaptosuccinic anhydride to form SH-D-GL; (b) reacting antigen with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-Antigen or with S-acetylmercaptosuccinic anhydride to form SH-Antigen; (c) reacting MB-D-GL with SH-Antigen or reacting SH-D-GL with MB-Antigen to form said conjugates of D-glutamic acid-D-lysine copolymer with antigen.
Claim 2
Dependent Claim
74 words
An improved method of preparing immunosuppressive conjugates of D-glutamic acid-D-lysine copolymer and an antigen, comprising the steps of: (a) reacting D-glutamic acid-D-lysine copolymer with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-D-GL or with S-acetylmercaptosuccinic anhydride to form SH-D-GL; (b) reacting antigen with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-Antigen or with S-acetylmercaptosuccinic anhydride to form SH-Antigen; (c) reacting MB-D-GL with SH-Antigen or reacting SH-D-GL with MB-Antigen to form said conjugates of D-glutamic acid-D-lysine copolymer with antigen.
Claim 3
Dependent Claim
74 words
An improved method for preparing conjugates of D-glutamic acid-D-lysine copolymer and insulin comprising the steps of: (a) reacting D-glutamic acid-D-lysine copolymer with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-D-GL or with S-acetylmercaptosuccinic anhydride to form SH-D-GL; (b) reacting insulin with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-In or with S-acetylmercaptosuccinic anhydride to form SH-In; and (c) reacting MB-D-GL with SH-In or reacting SH-D-GL with MB-In to form said conjugate of D-glutamic acid-D-lysine copolymer conjugate with insulin.
Claim 4
Dependent Claim
74 words
An improved method for preparing conjugates of D-glutamic acid-D-lysine copolymer and insulin comprising the steps of: (a) reacting D-glutamic acid-D-lysine copolymer with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-D-GL or with S-acetylmercaptosuccinic anhydride to form SH-D-GL; (b) reacting insulin with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-In or with S-acetylmercaptosuccinic anhydride to form SH-In; and (c) reacting MB-D-GL with SH-In or reacting SH-D-GL with MB-In to form said conjugate of D-glutamic acid-D-lysine copolymer conjugate with insulin.
Claim 5
Dependent Claim
80 words
An improved method for preparing conjugates of D-glutamic acid-D-lysine copolymer and ragweed antigen E comprising the steps of: (a) reacting D-glutamic acid-D-lysine copolymer with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-D-GL or with S-acetylmercaptosuccinic anhydride to form SH-D-GL; (b) reacting ragweed antigen E with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-AgE or with S-acetylmercaptosuccinic anhydride to form SH-AgE; and (c) reacting MB-D-GL with SH-AgE or reacting SH-D-GL with MB-AgE to form said conjugate of D-glutamic acid-D-lysine copolymer conjugate with ragweed antigen E.
Claim 6
Dependent Claim
80 words
An improved method for preparing conjugates of D-glutamic acid-D-lysine copolymer and ragweed antigen E comprising the steps of: (a) reacting D-glutamic acid-D-lysine copolymer with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-D-GL or with S-acetylmercaptosuccinic anhydride to form SH-D-GL; (b) reacting ragweed antigen E with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-AgE or with S-acetylmercaptosuccinic anhydride to form SH-AgE; and (c) reacting MB-D-GL with SH-AgE or reacting SH-D-GL with MB-AgE to form said conjugate of D-glutamic acid-D-lysine copolymer conjugate with ragweed antigen E.
Claim 7
Dependent Claim
74 words
An improved method of preparing immunosuppressive conjugates of D-glutamic acid-D-lysine copolymer and a protein, comprising the steps of: (a) reacting D-glutamic acid-D-lysine copolymer with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-D-GL or with S-acetylmercaptosuccinic anhydride to form SH-D-GL; (b) reacting protein with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-protein or with S-acetylmercaptosuccinic anhydride to form SH-protein; (c) reacting MB-D-GL with SH-protein or reacting SH-D-GL with MB-protein to form said conjugates of D-glutamic acid-D-lysine copolymer with protein.
Claim 8
Dependent Claim
74 words
An improved method of preparing immunosuppressive conjugates of D-glutamic acid-D-lysine copolymer and a protein, comprising the steps of: (a) reacting D-glutamic acid-D-lysine copolymer with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-D-GL or with S-acetylmercaptosuccinic anhydride to form SH-D-GL; (b) reacting protein with either m-maleimidobenzoyl-N-hydroxysuccinimide ester to form MB-protein or with S-acetylmercaptosuccinic anhydride to form SH-protein; (c) reacting MB-D-GL with SH-protein or reacting SH-D-GL with MB-protein to form said conjugates of D-glutamic acid-D-lysine copolymer with protein.
References Cited (Backward Citations)
US3794630A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-09-02 | 1984-03-03 | 1984-09-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-09-02 | 1988-03-03 | 1988-09-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-09-02 | 1992-03-03 | 1992-09-02 | $7700.00 | Window Closed / Lapsed |