Patent US4218335A successfully refreshed from Google Patents.
US4218335A
Expired / Lapsed
US
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Stabilizer for an immunochemical measuring reagent
Assignee: Mochida Pharmaceutical Co Ltd
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Filing: 1978-11-14
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Granted: 1980-08-19
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Expires: 1998-11-14
Abstract
Abstract A stabilizer consisting principally of cytochrome c or a thermally modified matter of cytochrome c for maintaining constant over an extended period the sensitivity of measurement and specificity of a measuring reagent on the basis of the immunochemical agglutination reaction and the agglutination inhibition reaction.
Inventors
- 👤 Ei Mochida
- 👤 Nobuhisa Ogawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 18:39
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54393; Y; Y10; Y10S; Y10S435/00; Y10S435/963; Y10S436/00; Y10S436/811; Y10S436/814; Y10S436/815; Y10S436/817; Y10S436/818; Y10S436/826; Y10S530/00; Y10S530/827; Y10S530/841
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
17 words
A stabilizer for immunochemical measuring reagents consisting of a buffer solution containing cytochrome c and sodium azide.
Claim 2
Dependent Claim
17 words
A stabilizer for immunochemical measuring reagents consisting of a buffer solution containing cytochrome c and sodium azide.
Claim 3
Dependent Claim
17 words
A stabilizer as set forth in claim 1 wherein the content of the cytochrome c is 0.01-5%.
Claim 4
Dependent Claim
17 words
A stabilizer as set forth in claim 1 wherein the content of the sodium azide is 0.05-0.2%.
Claim 5
Dependent Claim
24 words
A stabilizer as set forth in claim 1 wherein the cytochrome c is either cytochrome c of horse heart muscle or yeast cytochrome c.
Claim 6
Dependent Claim
40 words
A stabilizer for immunochemical measuring reagents consisting of a buffer solution and a solution obtained by heating a solution containing cytochrome c and sodium azide at 100°-130° C. for 2-8 hours in an autoclave and removing the produced coagulated substance.
Claim 7
Dependent Claim
40 words
A stabilizer for immunochemical measuring reagents consisting of a buffer solution and a solution obtained by heating a solution containing cytochrome c and sodium azide at 100°-130° C. for 2-8 hours in an autoclave and removing the produced coagulated substance.
Claim 8
Dependent Claim
17 words
A stabilizer as set forth in claim 5 wherein the content of the cytochrome c is 0.01-5%.
Claim 9
Dependent Claim
17 words
A stabilizer as set forth in claim 5 wherein the content of the sodium azide is 0.05-0.2%.
Claim 10
Dependent Claim
14 words
A buffer solution containing one of the stabilizers as in one of claims 1-7.
Claim 11
Dependent Claim
52 words
A process for producing a stabilizer for an immunochemical reagent consisting essentially of the steps of dissolving cytochrome c in a solution containing sodium azide, heating the thus obtained solution at 100°-130° C. for 2-8 hours in an autoclave, removing the coagulated substance from the solution and bufferizing the thus obtained solution.
Claim 12
Dependent Claim
52 words
A process for producing a stabilizer for an immunochemical reagent consisting essentially of the steps of dissolving cytochrome c in a solution containing sodium azide, heating the thus obtained solution at 100°-130° C. for 2-8 hours in an autoclave, removing the coagulated substance from the solution and bufferizing the thus obtained solution.
Claim 13
Dependent Claim
25 words
A process claimed in claim 9 wherein a buffering process comprises dialyzing the solution obtained in claim 9 for a night against a buffer solution.
Claim 14
Dependent Claim
25 words
A process claimed in claim 9 wherein the buffering process comprises adding to the solution obtained in claim 9 composite ingredients of a buffer solution.
Claim 15
Dependent Claim
56 words
A process for stabilizing an immunochemical measuring reagent consisting of carrier particles adsorbed with antigen or antibody, the stabilizing process comprising contacting cytochrome c and sodium azide with said carrier particles and allowing them to settle at 4°-10° C. for a night, centrifugally separating to obtain a precipitate, and suspending the precipitate in a buffer solution.
Claim 16
Dependent Claim
56 words
A process for stabilizing an immunochemical measuring reagent consisting of carrier particles adsorbed with antigen or antibody, the stabilizing process comprising contacting cytochrome c and sodium azide with said carrier particles and allowing them to settle at 4°-10° C. for a night, centrifugally separating to obtain a precipitate, and suspending the precipitate in a buffer solution.
Claim 17
Dependent Claim
32 words
An immunochemical measuring reagent consisting of carrier particles adsorbed with antigen or antibody, the immunochemical measuring reagent being stabilized by the presence of a buffered solution containing cytochrome c and sodium azide.
Claim 18
Dependent Claim
32 words
An immunochemical measuring reagent consisting of carrier particles adsorbed with antigen or antibody, the immunochemical measuring reagent being stabilized by the presence of a buffered solution containing cytochrome c and sodium azide.
Claim 19
Dependent Claim
57 words
A process for producing an immunochemical measuring reagent composed of carrier particles adsorbed with antigen or antibody, the process comprising contacting a solution containing 0.01-5.0% cytochrome c and 0.05-2% sodium azide with said carrier particles, settling them at 4°-10° C. for a night, centrifugally separating them to obtain precipitate, and suspending the precipitate in the buffer solution.
Claim 20
Dependent Claim
57 words
A process for producing an immunochemical measuring reagent composed of carrier particles adsorbed with antigen or antibody, the process comprising contacting a solution containing 0.01-5.0% cytochrome c and 0.05-2% sodium azide with said carrier particles, settling them at 4°-10° C. for a night, centrifugally separating them to obtain precipitate, and suspending the precipitate in the buffer solution.
Claim 21
Independent Claim
53 words
A method of maintaining, over an extended period of time, the sensitivity of measurement and specificity of a measuring reagent based on the immunochemical agglutination reaction or agglutination inhibition reaction which comprises adding to said measuring reagent a stabilizer consisting of a buffer solution containing 0.01-5% cytochrome c and 0.05- 2% sodium azide.
Claim 22
Independent Claim
53 words
A method of maintaining, over an extended period of time, the sensitivity of measurement and specificity of a measuring reagent based on the immunochemical agglutination reaction or agglutination inhibition reaction which comprises adding to said measuring reagent a stabilizer consisting of a buffer solution containing 0.01-5% cytochrome c and 0.05- 2% sodium azide.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-08-19 | 1984-02-18 | 1984-08-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-08-19 | 1988-02-18 | 1988-08-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-08-19 | 1992-02-18 | 1992-08-19 | $7700.00 | Window Closed / Lapsed |