Abstract Reagents for use in binding assays, particularly immunoassays, to determine diphenylhydantoin and for preparing reagents employed in such assays, including beta -galactosyl-umbelliferone-diphenylhydantoin conjugates, diphenylhydantoin immunogens, and N1, N3 and o-phenyl derivatives of diphenylhydantoin for preparing same. N1 and N3- omega -aminoalkyl and o-( omega -aminoalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to a beta -galactosyl-umbelliferone derivative to form labeled conjugates useful in homogeneous or heterogeneous binding assays. N1 and N3- omega -carboxyalkyl and o-( omega -carboxyalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to an immunogenic polyamino acid to form immunogen conjugates against which diphenylhydantoin-specific antibodies can be raised.
A diphenylhydantoin immunogen conjugate of the formula: ##STR14## wherein φ is phenyl, PAA is an immunogenic polypeptide or protein having a molecular weight of at least 5,000 bonded through an amide linkage, n=2 through 6, and p=1 through 50.
Claim 2
Dependent Claim
39 words
A diphenylhydantoin immunogen conjugate of the formula: ##STR14## wherein φ is phenyl, PAA is an immunogenic polypeptide or protein having a molecular weight of at least 5,000 bonded through an amide linkage, n=2 through 6, and p=1 through 50.
Claim 3
Dependent Claim
7 words
The conjugate of claim 1 wherein n=4.
Claim 4
Dependent Claim
15 words
The conjugate of claim 1 or 2 wherein said polypeptide or protein is an albumin.