Abstract The invention is in concern of a method for measuring the electrophoretic mobility of particles in a liquid test sample in order to improve the precision of the electrophoretic measurement and to reduce in particular the operational expenditures in such a method. By use of considerably simple means a very precise time measurement of the mobility of particles is obtained free from any subjective influences with respect to the selection of the particles to be evaluated and to the time measurement. Accordingly, signals variable with respect to their frequency and visualized on the screen of a monitor are derived from out of video synchronization pulses, said signals are logically combined to the digitalized adjustable image scanning signals, improved in their signal-to-noise ratio. The output signals of this combination effect a counting operation dependent on the particle mobility.
Method for measuring the electrophoretic mobility of particles comprising the steps of applying a voltage to a sample contained in an electrophoreses chamber including the particles to be measured, microscopically observing said sample, producing an image signal by a vidicon from said sample, said image signal including image synchronization pulses and line synchronization pulses, converting said image signal into an image of said sample on the screen of a monitor feeding the image signals into a filter line, subsequent thereto comparing said image signal to an adjustable reference voltage and converting thereby said image signal into a digitalised image signal, producing a first pulse obtained from the image synchronization pulse by adjustable delay and imaging said first pulse on the screen of said monitor, producing a second and a third pulse from out of the line synchronization pulse by adjustable delay and imaging said second and third pulse on the screen of the monitor, logically combining said first, second and third pulse and said digitalised image signal, and obtaining from the logical combination a first and a second signal, said first signal starting a counting operation, said second signal stopping said counting operation.
Claim 2
Dependent Claim
193 words
Method for measuring the electrophoretic mobility of particles comprising the steps of applying a voltage to a sample contained in an electrophoreses chamber including the particles to be measured, microscopically observing said sample, producing an image signal by a vidicon from said sample, said image signal including image synchronization pulses and line synchronization pulses, converting said image signal into an image of said sample on the screen of a monitor feeding the image signals into a filter line, subsequent thereto comparing said image signal to an adjustable reference voltage and converting thereby said image signal into a digitalised image signal, producing a first pulse obtained from the image synchronization pulse by adjustable delay and imaging said first pulse on the screen of said monitor, producing a second and a third pulse from out of the line synchronization pulse by adjustable delay and imaging said second and third pulse on the screen of the monitor, logically combining said first, second and third pulse and said digitalised image signal, and obtaining from the logical combination a first and a second signal, said first signal starting a counting operation, said second signal stopping said counting operation.