Patent US4209586A successfully refreshed from Google Patents.
US4209586A
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US
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Method of testing the effectiveness of a growth inhibiting agent on a microorganism
Assignee: HAYWARD CARROL B DUNWOODY
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Filing: 1977-07-14
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Granted: 1980-06-24
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Expires: 1997-07-14
Abstract
Abstract The changes in the redox potentials of cultures of a microorganism with and without a tested growth inhibiting agent are monitored during the phase of growth in which the redox potential is normally positive and the rate of potential change is approximately linear. Effective growth inhibiting agents produce a measurable decrease in the change of the redox potential to a more negative value within less than one hour. A sensible signal indicative of the growth inhibiting action of the tested agent may be obtained from a comparator by storing an amplified signal indicative of the redox potential at a first time and feeding the stored signal together with another amplified signal obtained less than one hour thereafter to the comparator.
Inventors
- 👤 Hans G. Noller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 03:18
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/18; C12M; C12M41/00; C12M41/28; C12M41/30; C12M41/36
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
134 words
A method of testing the effectiveness of a growth inhibiting agent on a microorganism which comprises: (a) introducing into a first batch of a nutrient medium an amount of the growth inhibiting agent to be tested; (b) introducing into said first batch and into a second batch of said nutrient medium respective inocula of said microorganism, said second batch being free of said agent and capable of supporting growth of said microorganism at a predetermined temperature; (c) incubating said first and second batches at said temperature; (d) sensing the redox potentials of the incubated batches at a predetermined time after said inoculating while said redox potentials vary at approximately linear rates; and (e) generating at least one sensible signal indicative of the difference between the sensed redox potentials as a measure of said effectiveness.
Claim 2
Independent Claim
134 words
A method of testing the effectiveness of a growth inhibiting agent on a microorganism which comprises: (a) introducing into a first batch of a nutrient medium an amount of the growth inhibiting agent to be tested; (b) introducing into said first batch and into a second batch of said nutrient medium respective inocula of said microorganism, said second batch being free of said agent and capable of supporting growth of said microorganism at a predetermined temperature; (c) incubating said first and second batches at said temperature; (d) sensing the redox potentials of the incubated batches at a predetermined time after said inoculating while said redox potentials vary at approximately linear rates; and (e) generating at least one sensible signal indicative of the difference between the sensed redox potentials as a measure of said effectiveness.
Claim 3
Dependent Claim
17 words
A method as set forth in claim 1, wherein said predetermined time is less than two hours.
Claim 4
Dependent Claim
22 words
A method as set forth in claim 2, wherein said predetermined time is less than one hour, said microorganism being a bacterium.
Claim 5
Dependent Claim
37 words
A method as set forth in claim 2, wherein said redox potentials are sensed while having a positive value as measured between a smooth platinum measuring electrode and a reference electrode, said nutrient medium containing dissolved oxygen.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-06-24 | 1983-12-24 | 1984-06-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-06-24 | 1987-12-24 | 1988-06-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-06-24 | 1991-12-24 | 1992-06-24 | $7700.00 | Window Closed / Lapsed |