Patent US4209372A successfully refreshed from Google Patents.
US4209372A
Expired / Lapsed
US
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Alkaline agarosse gel electrophoresis of hemoglobins
Assignee: Corning Glass Works
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Filing: 1979-06-01
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Granted: 1980-06-24
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Expires: 1999-06-01
Abstract
Abstract The common hemoglobin variants A, A 2 , F, S, and C are discretely separated by alkaline agarose gel electrophoresis employing (1) an agarose gel having a wet thickness of from about 0.1 to about 0.5 mm., (2) a gel buffer comprising tris(hydroxymethyl)aminomethane, ethylenediaminetetraacetic acid, and boric acid, and having an ionic strength of from about 0.065 to about 0.1 and a pH of from about 8.7 to about 9.1, (3) a well buffer comprising diethylbarbiturate and ethylenediaminetetraacetate and having an ionic strength of from about 0.01 to about 0.11 and a pH of from about 8.0 to about 9.5, and (4) a potential of from about 150 to about 300 volts.
Inventors
- 👤 Barry I. Bluestein
- 👤 Cyrus A. Lepp
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 17:53
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; B; B01; B01D; B01D57/00; B01D57/02; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
116 words
In a method for discretely separating the common hemoglobin variants A, A 2 , F, S, and C by electrophoresis, the improvement which comprises employing: A. an agarose gel having a wet thickness of from about 0.1 to about 0.5 mm, B. a gel buffer comprising tris(hydroxymethyl)aminomethane, ethylenediaminetetraacetic acid, and boric acid, and having an ionic strength of from about 0.065 to about 0.1 and a pH of from about 8.7 to about 9.1, C. a well buffer comprising diethylbarbiturate and ethylenediaminetetraacetate and having an ionic strength of from about 0.01 to about 0.11 and a pH of from about 8.0 to about 9.5, and D. a potential of from about 150 to about 300 volts.
Claim 2
Dependent Claim
116 words
In a method for discretely separating the common hemoglobin variants A, A 2 , F, S, and C by electrophoresis, the improvement which comprises employing: A. an agarose gel having a wet thickness of from about 0.1 to about 0.5 mm, B. a gel buffer comprising tris(hydroxymethyl)aminomethane, ethylenediaminetetraacetic acid, and boric acid, and having an ionic strength of from about 0.065 to about 0.1 and a pH of from about 8.7 to about 9.1, C. a well buffer comprising diethylbarbiturate and ethylenediaminetetraacetate and having an ionic strength of from about 0.01 to about 0.11 and a pH of from about 8.0 to about 9.5, and D. a potential of from about 150 to about 300 volts.
Claim 3
Dependent Claim
19 words
The method of claim 1 in which the gel wet thickness is from about 0.3 to about 0.4 mm.
Claim 4
Dependent Claim
21 words
The method of claim 1 in which the gel buffer has an ionic strength of from about 0.075 to about 0.090.
Claim 5
Dependent Claim
17 words
The method of claim 3 in which the gel buffer has an ionic strength of about 0.082.
Claim 6
Dependent Claim
20 words
The method of claim 4 in which the gel buffer has a pH of from about 8.8 to about 8.9.
Claim 7
Dependent Claim
29 words
The method of claim 5 in which the gel buffer is about 0.06 M consists essentially of 0.053 M tris(hydroxymethyl)aminomethane, 0.002 M ethylenediaminetetraacetic acid, and 0.0059 M boric acid.
Claim 8
Dependent Claim
20 words
The method of claim 1 in which the gel buffer has a pH of from about 8.8 to about 8.9.
Claim 9
Dependent Claim
21 words
The method of claim 1 in which the well buffer has an ionic strength of from about 0.04 to about 0.11.
Claim 10
Dependent Claim
17 words
The method of claim 8 in which the well buffer has an ionic strength of about 0.06.
Claim 11
Dependent Claim
20 words
The method of claim 9 in which the well buffer has a pH of from about 8.55 to about 8.65.
Claim 12
Dependent Claim
30 words
The method of claim 10 in which the well buffer is about 0.025 M and consists essentially of 0.025 M sodium diethylbarbiturate and about 0.017% weight per volume sodium ethylenediaminetetraacetate.
Claim 13
Dependent Claim
20 words
The method of claim 1 in which the well buffer has a pH of from about 8.5 to about 8.7.
Claim 14
Dependent Claim
17 words
The method of claim 1 in which the potential is from about 225 to about 270 volts.
Claim 15
Dependent Claim
13 words
The method of claim 1 in which the potential is about 250 volts.
Claim 16
Dependent Claim
24 words
The method of claim 1 in which the gel contains a humectant at a level of from 0 to about 10% weight per volume.
Claim 17
Dependent Claim
11 words
The method of claim 15 in which the humectant is sucrose.
Claim 18
Dependent Claim
20 words
The method of claim 16 in which the humectant is present at a level of about 5% weight per volume.
Claim 19
Dependent Claim
25 words
The method of claim 1 in which the gel contains a wetting agent at a level of from 0 to about 0.1% weight per volume.
Claim 20
Dependent Claim
13 words
The method of claim 18 in which the wetting agent is polyvinyl alcohol.
Claim 21
Dependent Claim
21 words
The method of claim 19 in which the wetting agent is present at a level of about 0.015% weight per volume.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-06-24 | 1983-12-24 | 1984-06-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-06-24 | 1987-12-24 | 1988-06-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-06-24 | 1991-12-24 | 1992-06-24 | $7700.00 | Window Closed / Lapsed |