US4206014A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for removing detergents from virus-antigen suspensions
Assignee: Siemens Healthcare Diagnostics GmbH Germany
•
Filing: 1978-11-07
•
Granted: 1980-06-03
•
Expires: 1998-11-07
Abstract
Abstract A method for isolating a detergent-free virus-antigen from a suspension of said virus-antigen containing detergent, which method comprises precipitating said virus-antigen by adding a polyethylene glycol to said suspension and then isolating the detergent-free virus-antigen precipitate by sedimentation, said detergent remaining in said suspension.
Inventors
- 👤 Edgar Reichert
- 👤 Mirko Majer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 13:42
CPC Classifications
C; C12; C12N; C12N7/00; A; A61; A61K; A61K39/00; A61K39/12; A61K39/145; A61K39/205; C12N2760/00; C12N2760/00011; C12N2760/16011; C12N2760/16111; C12N2760/16134; C12N2760/16151; C12N2760/16211; C12N2760/16234; C12N2760/16251; C12N2760/18011; C12N2760/18811; C12N2760/18834; C12N2760/18851; C12N2760/20011; C12N2760/20111; C12N2760/20134; C12N2760/20151; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S530/00; Y10S530/82; Y10S530/826
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
69 words
A method for isolating a detergent-free virus-antigen of an influenza virus, of a para-influenza virus, or of a rhabdo virus, from a suspension of said virus-antigen containing detergent, which method comprises precipitating said virus-antigen by adding 6 to 18 percent of polyethylene glycol having a molecular weight between 1000 and 40000 to said suspension and then isolating the detergent-free virus-antigen precipitate by sedimentation, said detergent remaining in said suspension.
Claim 2
Independent Claim
69 words
A method for isolating a detergent-free virus-antigen of an influenza virus, of a para-influenza virus, or of a rhabdo virus, from a suspension of said virus-antigen containing detergent, which method comprises precipitating said virus-antigen by adding 6 to 18 percent of polyethylene glycol having a molecular weight between 1000 and 40000 to said suspension and then isolating the detergent-free virus-antigen precipitate by sedimentation, said detergent remaining in said suspension.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein said virus is an influenza virus.
Claim 4
Dependent Claim
10 words
The method of claim 1 wherein said detergent is nonylphenoxypolyethoxyethanol.
Claim 5
Independent Claim
61 words
A method for isolating a virus-antigen of an influenza virus from a suspension of said virus-antigen containing nonylphenoxypolyethoxyethanol as a detergent, which method comprises precipitating said virus-antigen by adding 6 to 18 percent of polyethylene glycol having a molecular weight of about 6000 to said suspension and then isolating the detergent-free virus-antigen precipitate by sedimentation, said detergent remaining in said suspension.
Claim 6
Independent Claim
61 words
A method for isolating a virus-antigen of an influenza virus from a suspension of said virus-antigen containing nonylphenoxypolyethoxyethanol as a detergent, which method comprises precipitating said virus-antigen by adding 6 to 18 percent of polyethylene glycol having a molecular weight of about 6000 to said suspension and then isolating the detergent-free virus-antigen precipitate by sedimentation, said detergent remaining in said suspension.
Claim 7
Dependent Claim
16 words
The method of claim 1 wherein the virus-antigen precipitate is taken up in a liquid medium.
Claim 8
Dependent Claim
18 words
The method of claim 5 wherein water or an aqueous buffer solution is used as the liquid medium.
Claim 9
Dependent Claim
18 words
The method of claim 1 wherein the virus-antigens are precipitated by the addition of 12% of polyethylene glycol.
Claim 10
Dependent Claim
15 words
The method of claim 1 wherein the polyethylene glycol has a molecular weight of 6,000.
References Cited (Backward Citations)
US3415804A
Cited
US3989818A
Cited
US3790552A
Cited
US3962421A
Cited
US3951937A
Cited
US4064232A
Cited
US4029763A
Cited
US4118479A
Cited
US4118478A
Cited
US4113712A
Cited
US4102996A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-06-03 | 1983-12-03 | 1984-06-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-06-03 | 1987-12-03 | 1988-06-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-06-03 | 1991-12-03 | 1992-06-03 | $7700.00 | Window Closed / Lapsed |