US4064232A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for isolating the immunogenic components of influenza viruses
Assignee: Sandoz AG
•
Filing: 1976-05-18
•
Granted: 1977-12-20
•
Expires: 1996-05-18
Abstract
Abstract The immunogenic components from influenze viruses are isolated by selectively solubilizing the component in an aqueous media with a cationic detergent.
Inventors
- 👤 Helmut Bachmayer
- 👤 Gerhard Schmidt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 09:25
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/145; A61K2039/51; A61K2039/525; A61K2039/5252; C; C12; C12N; C12N2760/00; C12N2760/00011; C12N2760/16011; C12N2760/16111; C12N2760/16134; C12N2760/16211; C12N2760/16234
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
38 words
A method of isolating the hemagglutinin and neuramuridase components from influenza virus, comprising treating influenza virus in an aqueous medium with a cationic detergent to selectively solubilise components, and separating the resulting solubilised components from residual sub-viral particles.
Claim 2
Independent Claim
38 words
A method of isolating the hemagglutinin and neuramuridase components from influenza virus, comprising treating influenza virus in an aqueous medium with a cationic detergent to selectively solubilise components, and separating the resulting solubilised components from residual sub-viral particles.
Claim 3
Dependent Claim
33 words
A method according to claim 1, in which the influenza virus is an influenza Type A, A 1 , A 2 or B virus or a mixture of any two or more thereof.
Claim 4
Dependent Claim
29 words
A method according to claim 1, in which the influenza virus is strain A2/Aichi/68, MRC-2 (recombination of Type A2/England/42/72), MRC-11 (recombination of Type A2/Port Chalmers/73), A/Pasteur/30C (Mutagrip), or B/Mass/67.
Claim 5
Dependent Claim
25 words
A method according to claim 1, in which the cationic detergent is added to a virus concentrate having a pH of from 6.5 to 8.5.
Claim 6
Dependent Claim
23 words
A method according to claim 4, in which the virus concentrate is inactivated with formaldehyde prior to the addition of the cationic detergent.
Claim 7
Dependent Claim
20 words
A method according to claim 1, in which the cationic detergent is added in the form of an aqueous solution.
Claim 8
Dependent Claim
34 words
A method according to claim 1, in which the cationic detergent is added in such a quantity that the weight ratio of detergent to protein in the resulting mixture is from 1:2 to 1:10.
Claim 9
Dependent Claim
16 words
A method according to claim 7, in which the weight ratio is from 1:3 to 1:5.
Claim 10
Dependent Claim
34 words
A method according to claim 1, in which, after the addition of the cationic detergent, the resulting mixture is allowed to stand for 30 minutes to 16 hours at 4° C to 37° C.
Claim 11
Dependent Claim
22 words
A method according to claim 9, in which the mixture is allowed to stand for 30 to 60 minutes at room temperature.
Claim 12
Dependent Claim
18 words
A method according to claim 9, in which the mixture is allowed to stand overnight at 4° C.
Claim 13
Dependent Claim
114 words
A method according to claim 1, in which the cationic detergent is a compound of the formula, ##STR6## in which R 4 signifies alkyl or aryl, R 1 , R 2 and R 3 are the same or different and each signifies alkyl or aryl, or R 1 and R 2 , together with the nitrogen atom to which they are attached form a 5- or 6-membered saturated heterocyclic ring, and R 3 signifies alkyl or aryl, or R 1 , r 2 and R 3 , together with the nitrogen atom to which they are attached, signify a 5- or 6-membered heterocyclic ring, unsaturated at the nitrogen atom, and X signifies an anion.
Claim 14
Dependent Claim
73 words
A method according to claim 1, in which the cationic detergent is a compound of formula, ##STR7## in which X is an anion, and R 4 ' signifies alkyl of 8 to 22 carbon atoms, and either R 1 ' and R 2 ' are the same or different and each signifies methyl or alkyl of 8 to 22 carbon atoms, or R 1 ' signifies methyl and R 2 ' signifies benzyl.
Claim 15
Dependent Claim
36 words
A method according to claim 1, in which the cationic detergent is a compound of the formula ##STR8## in which R 4 ' signifies alkyl of 8 to 22 carbon atoms and X is an anion.
Claim 16
Dependent Claim
36 words
A method according to claim 1, in which the cationic detergent is a compound of the formula ##STR9## in which R 4 1 signifies alkyl of 8 to 22 carbon atoms and X is an anion.
Claim 17
Dependent Claim
40 words
A method according to claim 1, in which the cationic detergent is a compound of the formula, ##STR10## in which X is an anion, R 4 " signifies alkyl of 12 to 18 carbon atoms, and R 5 signifies hydrogen.
Claim 18
Dependent Claim
17 words
A method according to claim 1, in which the cationic detergent is a myristyltrimethylammonium or cetyltrimethylammonium salt.
Claim 19
Dependent Claim
14 words
A method according to claim 1, in which the cationic detergent is cetyltrimethylammonium bromide.
Claim 20
Dependent Claim
15 words
A method according to claim 1, in which the cationic detergent is a stearyldimethylbenzylammonium salt.
Claim 21
Dependent Claim
15 words
A method according to claim 1, in which the cationic detergent is a cetylpyridinium salt.
Claim 22
Dependent Claim
17 words
A method according to claim 1, in which the cationic detergent is a benzalkonium chloride or bromide.
Claim 23
Dependent Claim
16 words
A method according to claim 1, in which the cationic detergent is benzethonium or methylbenzethonium chloride.
Claim 24
Dependent Claim
14 words
A method according to claim 1, in which the cationic detergent is decamethonium chloride.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-12-19 | 1981-06-20 | 1981-12-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-12-19 | 1985-06-20 | 1985-12-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-12-19 | 1989-06-20 | 1989-12-20 | $7700.00 | Window Closed / Lapsed |