US4191809A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of measuring enzymatic activity using novel peptide derivatives
Assignee: Ajinomoto Co Inc
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Filing: 1978-09-20
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Granted: 1980-03-04
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Expires: 1998-09-20
Abstract
Abstract The compound 7-glycylprolylamino-4-methylcoumarin and certain of its derivatives are utilized for the diagnosis of pathological conditions such as hepatic diseases and gastric cancer by measuring the enzymatic activity of X-prolyl dipeptidyl-aminopeptidase.
Inventors
- 👤 Toshiharu Nagatsu
- 👤 Shumpei Sakakibara
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 08:52
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; C12Q2337/00; C12Q2337/20; C12Q2337/22; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/948
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
42 words
A method of measuring X-prolyl dipeptidyl-aminopeptidase activity which comprises contacting 7-glycyl-L-prolylamino-4-methylcoumarin or acid salts thereof with said X-prolyl dipeptidyl-aminopeptidase in an aqueous medium under conditions and for a period of time sufficient to effect enzymatic hydrolysis and assaying the enzymatically formed 7-amino-4-methylcoumarin.
Claim 2
Independent Claim
42 words
A method of measuring X-prolyl dipeptidyl-aminopeptidase activity which comprises contacting 7-glycyl-L-prolylamino-4-methylcoumarin or acid salts thereof with said X-prolyl dipeptidyl-aminopeptidase in an aqueous medium under conditions and for a period of time sufficient to effect enzymatic hydrolysis and assaying the enzymatically formed 7-amino-4-methylcoumarin.
Claim 3
Dependent Claim
22 words
The method of claim 1, wherein human cerebrospinal fluid, serum, saliva or urine is used as a source of said X-prolyl dipeptidyl-aminopeptidase.
Claim 4
Dependent Claim
7 words
The method of claim 1, utilizing 7-glycyl-L-prolylamino-4-methylcoumarin.
Claim 5
Dependent Claim
8 words
The method of claim 1, utilizing 7-glycyl-L-prolylamino-4-methylcoumarin p-toluenesulfonate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-03-04 | 1983-09-03 | 1984-03-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-03-04 | 1987-09-03 | 1988-03-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-03-04 | 1991-09-03 | 1992-03-04 | $7700.00 | Window Closed / Lapsed |