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US4191533A
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Pregnancy-specific β1 -glycoprotein and process for isolating it
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1977-12-09
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Granted: 1980-03-04
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Expires: 1997-12-09
Abstract
Abstract A pregnancy-specific β 1 -glycoprotein, characterized by an electrophoretic migration speed in agar gel in the range of the β 1 -globulins, a sedimentation constant of 4.6 S±0.5 S, a molecular weight of 100 000±15 000, an extinction coefficient E 1cm 1% of 11.6±0.5 (278 mμ; 1/15-molar phosphate buffer, pH 7.0), a carbohydrate content of 28.05±1.55%, comprising 10.7±1.0% of hexoses, 10.0±0.5% of hexosamine, 0.55±0.05% of fucose and 7.0±0.5% of neuraminic acid, and process for isolating it from the placenta or blood or urine of pregnant women. This protein may be used as a reagent and for the preparation of antisera as reagents for the detection of pregnancy and for pre-natal supervision.
Inventors
- 👤 Hans Bohn
- 👤 Ferdinand Stutzinger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 21:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/689; C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4715; C07K16/00; C07K16/18; G01N33/53; G01N33/531; G01N2800/00; G01N2800/36; G01N2800/368; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/814; Y10S530/00; Y10S530/806; Y10S530/827; Y10S530/829; Y10S530/834; Y10S530/85; Y10S530/851
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
134 words
A method for detecting the presence of a pregnancy-specific β 1 -glycoprotein in the blood or urine of a female animal or woman, whereby a pregnancy can be detected and supervised, which comprises contacting a sample of the blood or urine to be tested with antiserum to said pregnancy-specific β 1 -glycoprotein and examining the sample for evidence of an immune reaction, said pregnancy-specific β 1 -glycoprotein having an electrophoretic migration speed in agar gel in the range of β 1 -globulins, a sedimentation constant of 4.6 S±0.5 S, a molecular weight of about 100 000±15 000, an extinction coefficient E 1 cm 1% of 11.6±0.5 (278 mμ; 1/15-molar phosphate buffer, pH 7.0), and a carbohydrate content of 28.05±1.55%, comprising 10.7±1.0% of hexoses, 10.0±0.5% of hexosamine, 0.55±0.05% of fucose, and 7.0±0.5% of neuraminic acid.
Claim 2
Independent Claim
134 words
A method for detecting the presence of a pregnancy-specific β 1 -glycoprotein in the blood or urine of a female animal or woman, whereby a pregnancy can be detected and supervised, which comprises contacting a sample of the blood or urine to be tested with antiserum to said pregnancy-specific β 1 -glycoprotein and examining the sample for evidence of an immune reaction, said pregnancy-specific β 1 -glycoprotein having an electrophoretic migration speed in agar gel in the range of β 1 -globulins, a sedimentation constant of 4.6 S±0.5 S, a molecular weight of about 100 000±15 000, an extinction coefficient E 1 cm 1% of 11.6±0.5 (278 mμ; 1/15-molar phosphate buffer, pH 7.0), and a carbohydrate content of 28.05±1.55%, comprising 10.7±1.0% of hexoses, 10.0±0.5% of hexosamine, 0.55±0.05% of fucose, and 7.0±0.5% of neuraminic acid.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-03-04 | 1983-09-03 | 1984-03-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-03-04 | 1987-09-03 | 1988-03-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-03-04 | 1991-09-03 | 1992-03-04 | $7700.00 | Window Closed / Lapsed |