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Kit for determining the level of LDL cholesterol in body fluids

Assignee: Boston University
•
Filing: 1978-10-26
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Granted: 1980-02-26
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Expires: 1998-10-26
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Abstract

Abstract A kit for determining the level of LDL cholesterol in body fluids is disclosed which contains a plant lectin which is a specific agglutinating agent for LDL.

Inventors

  • 👤 Barry D. Sears

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-02 07:31
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/92

Claims Analysis

Total Claims: 8
Claim 1
Independent Claim 71 words
A diagnostic kit for detecting LDL cholesterol in a body fluid containing LDL cholesterol and another form of cholesterol selected from VLDL cholesterol, HDL cholesterol, and combinations thereof comprising, in combination: a. a reagent containing a lectin which is a specific agglutinating agent for LDL whereby LDL contained in said body fluid can be selectively agglutinated; and, b. a reagent for determining the amount of cholesterol in said specifically agglutinated LDL.
Claim 2
Dependent Claim 71 words
A diagnostic kit for detecting LDL cholesterol in a body fluid containing LDL cholesterol and another form of cholesterol selected from VLDL cholesterol, HDL cholesterol, and combinations thereof comprising, in combination: a. a reagent containing a lectin which is a specific agglutinating agent for LDL whereby LDL contained in said body fluid can be selectively agglutinated; and, b. a reagent for determining the amount of cholesterol in said specifically agglutinated LDL.
Claim 3
Dependent Claim 15 words
A diagnostic kit of claim 1 wherein said reagent containing a lectin has been lyophilized.
Claim 4
Dependent Claim 25 words
A diagnostic kit of claim 2 additionally containing a reconstituting reagent for said lyophilized lectin reagent, said reconstituting reagent containing an aqueous-based or water-misicible solvent.
Claim 5
Dependent Claim 16 words
A diagnostic kit of claim 3 additionally including a resolubilizing reagent for said specifically agglutinated LDL.
Claim 6
Dependent Claim 16 words
A diagnostic kit of claim 4 wherein said resolubilizing reagent comprises lactose in an aqueous solvent.
Claim 7
Dependent Claim 41 words
In a diagnostic kit for determining LDL cholesterol in a body fluid containing LDL cholesterol and another form of cholesterol, the improvement of including in said diagnostic kit a reagent containing a lectin which is a specific agglutinating agent for LDL.
Claim 8
Dependent Claim 41 words
In a diagnostic kit for determining LDL cholesterol in a body fluid containing LDL cholesterol and another form of cholesterol, the improvement of including in said diagnostic kit a reagent containing a lectin which is a specific agglutinating agent for LDL.

References Cited (Backward Citations)

No citation records indexed.

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1983-02-25 1983-08-27 1984-02-26 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1987-02-25 1987-08-27 1988-02-26 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1991-02-25 1991-08-27 1992-02-26 $7700.00 Window Closed / Lapsed

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