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US4189304A
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Device and method for detecting myoglobin
Assignee: Bayer Corp
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Filing: 1978-10-27
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Granted: 1980-02-19
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Expires: 1998-10-27
Abstract
Abstract A test device and method for detecting myoglobin in the presence of hemoglobin in a fluid test sample. The test device comprises a chromatographic medium incorporating serum, haptoglobin and/or antiserum which binds or retards hemoglobin while permitting myoglobin to traverse the length of the test device. A test sample of body fluid is placed in contact with an appropriate portion of the chromatographic medium of said test device; hemoglobin in the body fluid is retained or retarded by the serum, haptoglobin and/or antiserum; and myoglobin in the sample traverses the length of the medium and contacts reagent means at a predetermined location thereon, resulting in a detectable response thereat.
Inventors
- 👤 Ernest C. Adams, Jr.
- 👤 Kathleen M. Layman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 22:06
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; G01N33/523; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; G01N33/54391; B; B01; B01J; B01J2220/00; B01J2220/50; B01J2220/54; G01N30/00; G01N30/90; G01N30/92
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
66 words
A test device for detecting myoglobin in the presence of hemoglobin in a fluid test sample, said test device comprising a chromatographic medium having a sample contacting portion, a retaining portion, and a detection portion incorporating a reagent capable of producing a detectable response when contacted with myoglobin, wherein said retaining portion of said chromatographic medium incorporates serum, haptoglobin, antiserum for hemoglobin or a mixture thereof.
Claim 2
Dependent Claim
66 words
A test device for detecting myoglobin in the presence of hemoglobin in a fluid test sample, said test device comprising a chromatographic medium having a sample contacting portion, a retaining portion, and a detection portion incorporating a reagent capable of producing a detectable response when contacted with myoglobin, wherein said retaining portion of said chromatographic medium incorporates serum, haptoglobin, antiserum for hemoglobin or a mixture thereof.
Claim 3
Dependent Claim
22 words
The test device according to claim 1 in which the retaining portion of said chromatographic medium incorporates serum affixed to said medium.
Claim 4
Dependent Claim
22 words
The test device according to claim 1 in which the retaining portion of said chromatographic medium incorporates haptoglobin affixed to said medium.
Claim 5
Dependent Claim
24 words
The test device according to claim 1 in which the retaining portion of said chromatographic medium incorporates antiserum for hemoglobin affixed to said medium.
Claim 6
Dependent Claim
20 words
The test device according to claim 1 in which the detectable response produced by the reagent is a color change.
Claim 7
Dependent Claim
74 words
A test device for detecting myoglobin in the presence of hemoglobin in a fluid test sample, said test device comprising a chromatographic medium having a sample contacting portion, a retaining portion, and a detection portion, wherein said retaining portion of said chromatographic medium incorporates serum, haptoglobin, antiserum for hemoglobin or a mixture thereof affixed to said medium and wherein said detection portion of said chromatographic medium incorporates a reagent for detection of peroxidase-like substances.
Claim 8
Dependent Claim
74 words
A test device for detecting myoglobin in the presence of hemoglobin in a fluid test sample, said test device comprising a chromatographic medium having a sample contacting portion, a retaining portion, and a detection portion, wherein said retaining portion of said chromatographic medium incorporates serum, haptoglobin, antiserum for hemoglobin or a mixture thereof affixed to said medium and wherein said detection portion of said chromatographic medium incorporates a reagent for detection of peroxidase-like substances.
Claim 9
Dependent Claim
145 words
A test device for the detection of myoglobin in a test sample containing hemoglobin, which comprises: a length of capillary matrix having a first and second end; a first zone located in said length of capillary matrix extending from the first end of the matrix for contact with a test sample; a second zone located in said length of capillary matrix in the direction of said second end from said first zone; a first reagent incorporated with said matrix in said second zone and adapted to slow or prevent capillary movement through said second zone of hemoglobin present in the test sample; a third zone located in said length of capillary matrix extending from the second end toward the second zone; and a second reagent incorporated with said matrix in said third zone and a adapted to provide a detectable response when contacted with myoglobin.
Claim 10
Dependent Claim
145 words
A test device for the detection of myoglobin in a test sample containing hemoglobin, which comprises: a length of capillary matrix having a first and second end; a first zone located in said length of capillary matrix extending from the first end of the matrix for contact with a test sample; a second zone located in said length of capillary matrix in the direction of said second end from said first zone; a first reagent incorporated with said matrix in said second zone and adapted to slow or prevent capillary movement through said second zone of hemoglobin present in the test sample; a third zone located in said length of capillary matrix extending from the second end toward the second zone; and a second reagent incorporated with said matrix in said third zone and a adapted to provide a detectable response when contacted with myoglobin.
Claim 11
Dependent Claim
13 words
The test device of claim 7 in which the first reagent is haptoglobin.
Claim 12
Dependent Claim
13 words
The test device of claim 7 in which the first reagent is serum.
Claim 13
Dependent Claim
53 words
A method for detecting myoglobin in the presence of hemoglobin in a fluid test sample, comprising contacting the sample contacting portion of the test device of claim 1 with a fluid test sample containing hemoglobin and suspected of containing myoglobin, and observing any detectable response in the detection portion of said test device.
Claim 14
Dependent Claim
53 words
A method for detecting myoglobin in the presence of hemoglobin in a fluid test sample, comprising contacting the sample contacting portion of the test device of claim 5 with a fluid test sample containing hemoglobin and suspected of containing myoglobin, and observing any color change in the detection portion of said test device.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-02-18 | 1983-08-20 | 1984-02-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-02-18 | 1987-08-20 | 1988-02-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-02-18 | 1991-08-20 | 1992-02-19 | $7700.00 | Window Closed / Lapsed |