Patent US4186251A successfully refreshed from Google Patents.
US4186251A
Expired / Lapsed
US
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Composition and method for determination of cholesterol
Assignee: Bayer Corp
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Filing: 1973-03-01
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Granted: 1980-01-29
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Expires: 1993-03-01
Abstract
Abstract The present invention relates to compositions and methods for use in determining free and total cholesterol levels in fluids such as body fluids. A test for free cholesterol is disclosed based on the determination of hydrogen peroxide released through the action of a chemical system having cholesterol oxidase activity on free cholesterol. A preferred means for determining the released hydrogen peroxide involves the use of a substance having peroxidative activity and an oxidation-reduction indicator. The addition of a chemical system having cholesterol ester hydrolase activity to the free cholesterol test composition provides an integral test composition for the determination of total cholesterol.
Inventors
- 👤 Peter N. Tarbutton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 19:25
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/60; C12N; C12N9/00; C12N9/14; C12N9/16; C12N9/18; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/863; Y10S435/872
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
67 words
A test device for use in determining total cholesterol in a biological fluid sample comprising a test composition which includes a chemical system having cholesterol ester hydrolase activity, a chemical system having cholesterol oxidase activity, and means for determining at least one of the reaction products produced when free cholesterol is contacted with said chemical system having cholesterol oxidase activity; and a carrier for said test composition.
Claim 2
Dependent Claim
67 words
A test device for use in determining total cholesterol in a biological fluid sample comprising a test composition which includes a chemical system having cholesterol ester hydrolase activity, a chemical system having cholesterol oxidase activity, and means for determining at least one of the reaction products produced when free cholesterol is contacted with said chemical system having cholesterol oxidase activity; and a carrier for said test composition.
Claim 3
Dependent Claim
17 words
A test device as in claim 1 wherein said carrier is absorbent relative to said fluid sample.
Claim 4
Dependent Claim
16 words
A test device as in claim 1 wherein said test composition additionally includes a buffering material.
Claim 5
Dependent Claim
26 words
A test device as in claim 1 wherein said chemical system having cholesterol ester hydrolase activity comprises cholesterol ester hydrolase and a biliary enzyme co-factor therefor.
Claim 6
Dependent Claim
70 words
A test device as in claim 1 wherein said means for determining at least one of the reaction products produced when free cholesterol is contacted with said chemical system having cholesterol oxidase activity comprises means for determining hydrogen peroxide which means comprises a substance having peroxidative activity and an indicator material which is oxidized in the presence of peroxide and said substance having peroxidative activity and which changes color thereupon.
References Cited (Backward Citations)
US3099605A
Cited
US3092465A
Cited
US3183173A
Cited
US3607093A
Cited
FR2047147A5
Cited
US3776816A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-01-28 | 1983-07-30 | 1984-01-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-01-28 | 1987-07-30 | 1988-01-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-01-28 | 1991-07-30 | 1992-01-29 | $7700.00 | Window Closed / Lapsed |