Patent US4177188A successfully refreshed from Google Patents.
US4177188A
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Process for recovering purified albumin from blood plasma using PEG and caprylic acid
Assignee: Nordisk Insulinlaboratorium
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Filing: 1978-01-10
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Granted: 1979-12-04
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Expires: 1998-01-10
Abstract
Abstract A process is disclosed for the recovery of albumin in very high purity from human blood plasma by treatment of the blood plasma with polyethylene glycol (PEG) to precipitate out impurities followed by thermocoagulation of a resulting supernatant aqueous solution containing PEG and albumin in the presence of caprylic acid and isolation of the purified albumin by precipitation.
Inventors
- 👤 Jorgen F. Hansen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:53
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/76; C07K14/765; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
145 words
A process for recovering purified albumin suitable for intravenous injection from human blood plasma without denaturing the globulins present therein, comprising the steps of: (a) mixing the blood plasma with 150 to 300 g polyethylene glycol (PEG) per liter of liquid mixture and adjusting the mixture to a pH of 6 to 7 for precipitating fibrinogen and globulins while forming a supernatant containing albumin; (b) separating the supernatant from the formed precipitate; (c) adjusting the pH of the supernatant to a pH of 4.5 to 5.3; for precipitating albumin; (d) redissolving the albumin with entrained PEG in an aqueous medium; (e) subjecting the resulting albumin solution formed during step (d) to thermocoagulation at 50 to 65° C. and a pH of 4.6 to 5.0 in the presence of a stabilzing amount of caprylic acid; and (f) isolating the purified albumin from the solution by precipitation.
Claim 2
Dependent Claim
145 words
A process for recovering purified albumin suitable for intravenous injection from human blood plasma without denaturing the globulins present therein, comprising the steps of: (a) mixing the blood plasma with 150 to 300 g polyethylene glycol (PEG) per liter of liquid mixture and adjusting the mixture to a pH of 6 to 7 for precipitating fibrinogen and globulins while forming a supernatant containing albumin; (b) separating the supernatant from the formed precipitate; (c) adjusting the pH of the supernatant to a pH of 4.5 to 5.3; for precipitating albumin; (d) redissolving the albumin with entrained PEG in an aqueous medium; (e) subjecting the resulting albumin solution formed during step (d) to thermocoagulation at 50 to 65° C. and a pH of 4.6 to 5.0 in the presence of a stabilzing amount of caprylic acid; and (f) isolating the purified albumin from the solution by precipitation.
Claim 3
Dependent Claim
29 words
A process according to claim 1, wherein PEG is used in an amount of 180 to 200 g PEG per liter liquid supernatant obtained after precipitating fibrinogen and globulins.
Claim 4
Dependent Claim
32 words
A process according to claim 1, wherein the caprylic acid is used in an amount of 2 to 3 g caprylic acid per liter of the solution of albumin during the thermocoagulation.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-12-03 | 1983-06-04 | 1983-12-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-12-03 | 1987-06-04 | 1987-12-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-12-03 | 1991-06-04 | 1991-12-04 | $7700.00 | Window Closed / Lapsed |