US4166844A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Solid phase separation technique for use in radioimmunoassays
Assignee: Bracco International BV
•
Filing: 1977-04-18
•
Granted: 1979-09-04
•
Expires: 1997-04-18
Abstract
Abstract A radioimmunoassay procedure, and article of manufacture for carrying out that procedure, are disclosed herein. The solid phase separation technique utilized in the radioimmunoassay of this invention utilizes a test tube, the internal surface of which has been coated with two antibody layers.
Inventors
- 👤 Jan-I Tu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 11:51
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54306; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/81; Y10S436/826
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
61 words
A double antibody-coated test tube comprising a test tube capable of binding a layer of antibodies, having bound to at least a portion of its internal surface a layer of nonspecific antibodies and having bound to the layer of nonspecific antibodies a layer or specific antibodies, wherein the layer of specific antibodies is chemically linked to the layer of nonspecific antibodies.
Claim 2
Dependent Claim
61 words
A double antibody-coated test tube comprising a test tube capable of binding a layer of antibodies, having bound to at least a portion of its internal surface a layer of nonspecific antibodies and having bound to the layer of nonspecific antibodies a layer or specific antibodies, wherein the layer of specific antibodies is chemically linked to the layer of nonspecific antibodies.
Claim 3
Dependent Claim
23 words
A double antibody-coated test tube in accordance with claim 1 wherein the layer of nonspecific antibodies is physically adsorbed on the test tube.
Claim 4
Dependent Claim
18 words
A double antibody-coated test tube in accordance with claim 1 wherein the test tube is made of polystyrene.
Claim 5
Dependent Claim
187 words
A process for the determination of an antigen unknown comprising: (i) incubating a mixture of radioisotope-labelled antigen and unknown sample in a double antibody-coated test tube comprising a test tube capable of binding a layer of antibodies, having bound to at least a portion of its internal surface a layer of antibodies that is substantially non-binding with the antigen unknown, and having chemically linked to the first layer of antibodies a second layer of antibodies, the antibodies in said second layer being capable of binding with the antigen unknown; (ii) separating the radioisotope-labelled antigen bound to the double antibody-coated test tube from the free radioisotope-labelled antigen; (iii) determining the radioactivity of the bound radioisotope-labelled antigen, the radioactivity of the free radioisotope-labelled antigen or the radioactivity of each; (iv) referencing the amount of bound radioisotope-labelled antigen, the amount of free radioisotope-labelled antigen or the ratio of bound/free or free/bound radioisotope-labelled antigen to corresponding values obtained by carrying out steps (i), (ii) and (iii) using as a sample known amounts of antigen; and (v) determining the amount of antigen in the unknown sample using the relationship established in (iv).
Claim 6
Dependent Claim
187 words
A process for the determination of an antigen unknown comprising: (i) incubating a mixture of radioisotope-labelled antigen and unknown sample in a double antibody-coated test tube comprising a test tube capable of binding a layer of antibodies, having bound to at least a portion of its internal surface a layer of antibodies that is substantially non-binding with the antigen unknown, and having chemically linked to the first layer of antibodies a second layer of antibodies, the antibodies in said second layer being capable of binding with the antigen unknown; (ii) separating the radioisotope-labelled antigen bound to the double antibody-coated test tube from the free radioisotope-labelled antigen; (iii) determining the radioactivity of the bound radioisotope-labelled antigen, the radioactivity of the free radioisotope-labelled antigen or the radioactivity of each; (iv) referencing the amount of bound radioisotope-labelled antigen, the amount of free radioisotope-labelled antigen or the ratio of bound/free or free/bound radioisotope-labelled antigen to corresponding values obtained by carrying out steps (i), (ii) and (iii) using as a sample known amounts of antigen; and (v) determining the amount of antigen in the unknown sample using the relationship established in (iv).
References Cited (Backward Citations)
US3720760A
Cited
US3720760B1
Cited
US3646346A
Cited
US3790663A
Cited
US3904367A
Cited
US3960489A
Cited
US3979509A
Cited
US4017597A
Cited
US4069352A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-09-03 | 1983-03-05 | 1983-09-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-09-03 | 1987-03-05 | 1987-09-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-09-03 | 1991-03-05 | 1991-09-04 | $7700.00 | Window Closed / Lapsed |