Patent US4152210A successfully refreshed from Google Patents.
US4152210A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biologically active materials attached to a ferromagnetic support
Assignee: Beecham Group PLC
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Filing: 1975-04-30
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Granted: 1979-05-01
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Expires: 1995-04-30
Abstract
Abstract Biologically active materials such as enzymes attached to a ferromagnetic support such as iron or iron oxide particles enable a magnetic separation of the supported biologically active material from a reaction medium after carrying out a biochemical reaction.
Inventors
- 👤 Peter J. Robinson
- 👤 Peter Dunnill
- 👤 Malcolm D. Lilly
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 22:06
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; C12P; C12P1/00; C12P13/00; C12P13/04; C12P13/22; C12P13/225
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
35 words
A biochemically reactive substance which comprises an enzyme attached to a solid iron or iron oxide ferromagnetic support in particulate form with the support particles having a particle diameter of from 5 to 1000 microns.
Claim 2
Dependent Claim
35 words
A biochemically reactive substance which comprises an enzyme attached to a solid iron or iron oxide ferromagnetic support in particulate form with the support particles having a particle diameter of from 5 to 1000 microns.
Claim 3
Dependent Claim
21 words
A biochemically reactive substance according to claim 1 in which the particles have a diameter of from 10 to 100 microns.
Claim 4
Dependent Claim
27 words
A biochemically reactive substance according to claim 1 in which the enzyme is attached to an organic polymeric material which is itself attached to the ferromagnetic support.
Claim 5
Dependent Claim
32 words
A biochemically reactive substance according to claim 3 in which the support is formed into a composite material with an organic polymeric material and the enzyme is attached to the polymeric material.
Claim 6
Dependent Claim
32 words
A biochemically reactive substance according to claim 3 in which the support is coated with a film of an organic polymeric material and the enzyme is attached to the organic polymeric material.
Claim 7
Dependent Claim
20 words
A biochemically reactive substance according to claim 3 in which the enzyme is chemically attached to the organic polymeric material.
Claim 8
Dependent Claim
30 words
A biochemically reactive substance according to claim 1 in which the ferromagnetic support having a surface layer of oxide is chemically attached to the enzyme by the use of 3-aminopropyltriethoxysilane.
Claim 9
Dependent Claim
106 words
A process for carrying out a biochemical reaction comprising the steps of: (a) contacting an enzyme attached to a particulate iron or iron oxide ferromagnetic support, with the support particles having a particle diameter of from 5 to 1000 microns, with a reaction medium in a reaction vessel to effect a biochemical reaction; (b) applying a magnetic field to the reaction medium and the enzyme attached to the particulate ferromagnetic support sufficient to retain the enzyme within the reaction vessel; and (c) removing the reaction medium after the reaction is completed from the reaction vessel, thereby effecting a separation of the enzyme from the reaction medium.
Claim 10
Dependent Claim
106 words
A process for carrying out a biochemical reaction comprising the steps of: (a) contacting an enzyme attached to a particulate iron or iron oxide ferromagnetic support, with the support particles having a particle diameter of from 5 to 1000 microns, with a reaction medium in a reaction vessel to effect a biochemical reaction; (b) applying a magnetic field to the reaction medium and the enzyme attached to the particulate ferromagnetic support sufficient to retain the enzyme within the reaction vessel; and (c) removing the reaction medium after the reaction is completed from the reaction vessel, thereby effecting a separation of the enzyme from the reaction medium.
Claim 11
Dependent Claim
19 words
A process according to claim 8 in which the particles have a diameter of from 10 to 100 microns.
Claim 12
Dependent Claim
25 words
A process according to claim 8 in which the enzyme is attached to an organic polymeric material which is itself attached to the ferromagnetic support.
Claim 13
Dependent Claim
30 words
A process according to claim 10 in which the support is formed into a composite material with the organic polymeric material and the enzyme is attached to the polymeric material.
Claim 14
Dependent Claim
30 words
A process according to claim 10 in which the support is coated with a film of the organic polymeric material and the enzyme is attached to the organic polymeric material.
Claim 15
Dependent Claim
18 words
A process according to claim 10 in which the enzyme is chemically attached to the organic polymeric material.
Claim 16
Dependent Claim
28 words
A process according to claim 8 in which the ferromagnetic support having a surface layer of oxide is chemically attached to the enzyme by the use of 3-aminopropyltriethoxysilane.
Claim 17
Dependent Claim
25 words
A process according to claim 8 in which the support is a particulate support which is magnetised by the use of an alternating magnetic field.
Claim 18
Dependent Claim
22 words
A process according to claim 8 in which the magnetic field is applied in step (b) after the reaction has been completed.
Claim 19
Dependent Claim
37 words
A process according to claim 8 in which the reaction medium is continuously flowed through the reaction vessel and the magnetic field is applied throughout the reaction so as to retain the enzyme in the reaction vessel.
Claim 20
Dependent Claim
129 words
A process for carrying out a biochemical reaction comprising the steps of: (a) contacting an enzyme attached to a particulate iron or iron oxide ferromagnetic support, with the support particles having a particle diameter of from 5 to 1000 microns, with a reaction medium to form a reaction mixture in a reaction vessel to effect a biochemical reaction; (b) applying a magnetic field to the reaction mixture and stirring the reaction mixture; (c) discontinuing stirring of the reaction mixture after the reaction has been completed, but retaining the magnetic field while allowing the magnetized particles to flocculate and settle to the bottom of the reaction vessel; and (d) removing the enzyme from the bottom of the reaction vessel thereby effecting a separation of the enzyme from the reaction medium.
Claim 21
Dependent Claim
129 words
A process for carrying out a biochemical reaction comprising the steps of: (a) contacting an enzyme attached to a particulate iron or iron oxide ferromagnetic support, with the support particles having a particle diameter of from 5 to 1000 microns, with a reaction medium to form a reaction mixture in a reaction vessel to effect a biochemical reaction; (b) applying a magnetic field to the reaction mixture and stirring the reaction mixture; (c) discontinuing stirring of the reaction mixture after the reaction has been completed, but retaining the magnetic field while allowing the magnetized particles to flocculate and settle to the bottom of the reaction vessel; and (d) removing the enzyme from the bottom of the reaction vessel thereby effecting a separation of the enzyme from the reaction medium.
Claim 22
Dependent Claim
16 words
A process according to claim 18 in which the bottom of the reaction vessel is magnetized.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-04-30 | 1982-10-30 | 1983-05-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-04-30 | 1986-10-30 | 1987-05-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-04-30 | 1990-10-30 | 1991-05-01 | $7700.00 | Window Closed / Lapsed |