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Patent US4148888A successfully refreshed from Google Patents.
US4148888A
Expired / Lapsed
US
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3-Deazaadenosine as an inhibitor of adenosylhomocysteine hydrolase with antiviral activity
Assignee: United States Department of Health and Human Services
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Filing: 1978-03-13
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Granted: 1979-04-10
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Expires: 1998-03-13
Abstract
Abstract 3-Deazaadenosine has been found to be an effective antiviral and antifocal agent in tissue culture of animals as evidenced by its activity against Rous sarcoma virus, influenza virus, vesicular stomatitis virus, Sindbis virus, and Newcastle disease virus in the range 0.03-0.3 mM. This compound has also shown use as an inhibitor of adenosylhomocysteine hydrolase from beef liver in the range of 0.001-0.008 mM (I50). The antiviral and antifocal effect is correlated with the inhibition of hydrolysis of adenosylhomocysteine in cells. This inhibition of adenosylhomocysteine hydrolase can also be demonstrated in livers of rats injected with 3-deazaadenosine.
Inventors
- 👤 Giulio L. Cantoni
- 👤 Peter K. Chiang
- 👤 Henry H. Richards
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 16:22
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/70
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
25 words
A method of producing an antiviral effect in a tissue culture system comprising applying to said tissue culture system an effective antiviral amount of 3-deazaadenosine.
Claim 2
Independent Claim
25 words
A method of producing an antiviral effect in a tissue culture system comprising applying to said tissue culture system an effective antiviral amount of 3-deazaadenosine.
Claim 3
Dependent Claim
18 words
The method according to claim 1 wherein the antiviral amount utilized is in the range of 0.03-0.3 mM.
Claim 4
Dependent Claim
25 words
The method according to claim 1 wherein 3-deazaadenosine is utilized in a tissue culture system of chick embryo cells mixed with Rous sarcoma virus (RSV-BH).
Claim 5
Dependent Claim
49 words
The method according to claim 1 wherein 3-deazaadenosine is utilized in a tissue culture system wherein the chick embryo is mixed with a cytolytic RNA virus selected from a member of the group consisting of vesicular stomatitis virus, Sindbis virus, and Newcastle disease virus to inhibit virus focal formation.
Claim 6
Dependent Claim
35 words
The method according to claim 1 wherein 3-deazaadenosine is utilized in a tissue culture system of Rhesus monkey kidney culture mixed with A/Vic/3/75 (H3N2) wild type influenza virus and the virus focal formation is inhibited.
Claim 7
Independent Claim
28 words
A method of inhibiting adenosylhomocysteine hydrolase comprising administering to a rat an effective amount of from 100-200 mg/kg of body weight of 3-deazaadenosine to inhibit said adenosylhomocysteine hydrolase.
Claim 8
Independent Claim
28 words
A method of inhibiting adenosylhomocysteine hydrolase comprising administering to a rat an effective amount of from 100-200 mg/kg of body weight of 3-deazaadenosine to inhibit said adenosylhomocysteine hydrolase.
Claim 9
Dependent Claim
36 words
An enzyme inhibitor system consisting essentially of adenosylhomocysteine hydrolase of beef liver and 3-deazaadenosine as an active ingredient in effective amounts of from 0.001 to 0.008 mM(I 50 ) as an inhibitor of said adenosylhomocysteine hydrolase.
Claim 10
Dependent Claim
36 words
An enzyme inhibitor system consisting essentially of adenosylhomocysteine hydrolase of beef liver and 3-deazaadenosine as an active ingredient in effective amounts of from 0.001 to 0.008 mM(I 50 ) as an inhibitor of said adenosylhomocysteine hydrolase.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-04-09 | 1982-10-09 | 1983-04-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-04-09 | 1986-10-09 | 1987-04-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-04-09 | 1990-10-09 | 1991-04-10 | $7700.00 | Window Closed / Lapsed |