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US4145185A
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Reagents for screening tests and bioassay of von Willebrand's factor (platelet aggregating factor) in blood plasmas
Assignee: RTI International
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Filing: 1977-02-25
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Granted: 1979-03-20
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Expires: 1997-02-25
Abstract
Abstract Screening tests and bioassay of von Willebrand's factor (platelet aggregating factor) in human and animal blood plasma are effected using a reagent of blood platelets and snake venom having a positive platelet aggregating cofactor effect. The reagent and tests may also suitably employ dried blood platelets. The reagent may comprise dried platelets and either ristocetin or active snake venom as the platelet aggregating cofactor.
Inventors
- 👤 Kenneth M. Brinkhous
- 👤 Marjorie S. Read
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 12:41
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
34 words
A test reagent for the bioassay of von Willebrand's factor and platelet aggregating factor in blood plasmas comprising blood platelets and a snake venom characterized as having a positive cofactor effect for platelet aggregation.
Claim 2
Dependent Claim
34 words
A test reagent for the bioassay of von Willebrand's factor and platelet aggregating factor in blood plasmas comprising blood platelets and a snake venom characterized as having a positive cofactor effect for platelet aggregation.
Claim 3
Dependent Claim
35 words
The test reagent of claim 1 wherein said venom is obtained from a snake selected from the species Bothrops jararaca, Bothrops alternatus, Bothrops neowiidis, Trimeresurus flavoviridis, A. rhodostoma, Bothrops atrox, Bothrops jararacussu, and Bothrops lansbergii.
Claim 4
Dependent Claim
23 words
The test reagent of claim 2 wherein said species is selected from Bothrops jararaca, Bothrops alternatus, Bothrops neowiidis, Trimeresurus flavoviridis, and A. rhodostoma.
Claim 5
Dependent Claim
19 words
The test reagent of claim 2 wherein said species is selected from Bothrops jararaca, Bothrops alternatus, and Bothrops neowiidis.
Claim 6
Dependent Claim
13 words
The test reagent of claim 1 wherein said venom is Bothrops jararaca venom.
Claim 7
Dependent Claim
11 words
The test reagent of claim 1 wherein said platelets are fixed.
Claim 8
Dependent Claim
11 words
The test reagent of claim 6 wherein said platelets are dried.
Claim 9
Dependent Claim
42 words
A test reagent for the bioassay of von Willebrand's factor and platelet aggregating factor in human and animal plasmas comprising fixed-dried blood platelets and, as a cofactor for platelet aggregation, a snake venom characterized as having a positive cofactor effect, or ristocetin.
Claim 10
Dependent Claim
42 words
A test reagent for the bioassay of von Willebrand's factor and platelet aggregating factor in human and animal plasmas comprising fixed-dried blood platelets and, as a cofactor for platelet aggregation, a snake venom characterized as having a positive cofactor effect, or ristocetin.
Claim 11
Dependent Claim
11 words
The test reagent of claim 8 wherein said platelets are airdried.
Claim 12
Dependent Claim
11 words
The test reagent of claim 8 wherein said platelets are lyophilized.
Claim 13
Dependent Claim
38 words
A procedure for the bioassay of von Willebrand's factor in blood plasma which comprises determining the rate of aggregation of a platelet suspension effected by adding human or animal blood plasma to the test reagent of claim 1.
Claim 14
Dependent Claim
35 words
A procedure for the bioassay of von Willebrand's factor in blood plasma which comprises determining the rate of aggregation of a platelet suspension effected by adding blood plasma to the test reagent of claim 8.
Claim 15
Dependent Claim
54 words
In a method of determining the degree of platelet aggregation in blood plasma wherein a suspension of blood platelets and a cofactor for platelet aggregation are a dded to the plasma to be tested, the improvement which comprises using snake venom characterized as having a positive cofactor effect as a cofactor for platelet aggregation.
Claim 16
Dependent Claim
54 words
In a method of determining the degree of platelet aggregation in blood plasma wherein a suspension of blood platelets and a cofactor for platelet aggregation are a dded to the plasma to be tested, the improvement which comprises using snake venom characterized as having a positive cofactor effect as a cofactor for platelet aggregation.
Claim 17
Dependent Claim
50 words
In a method of determining the degree of platelet aggregation in human and animal plasma wherein a suspension of blood platelets and a positive cofactor for platelet aggregation are added to the plasma to be tested, the improvement which comprises using blood platelets which have been air dried or lyophilized.
Claim 18
Dependent Claim
50 words
In a method of determining the degree of platelet aggregation in human and animal plasma wherein a suspension of blood platelets and a positive cofactor for platelet aggregation are added to the plasma to be tested, the improvement which comprises using blood platelets which have been air dried or lyophilized.
Claim 19
Dependent Claim
47 words
A procedure for the bioassay of von Willebrand's factor in blood plasma which comprises determining the rate of aggregation of a platelet suspension by adding to human or animal blood plasma blood platelets and a snake venom characterized as having a positive cofactor effect for platelet aggregation.
Claim 20
Dependent Claim
47 words
A procedure for the bioassay of von Willebrand's factor in blood plasma which comprises determining the rate of aggregation of a platelet suspension by adding to human or animal blood plasma blood platelets and a snake venom characterized as having a positive cofactor effect for platelet aggregation.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-03-19 | 1982-09-18 | 1983-03-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-03-19 | 1986-09-18 | 1987-03-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-03-19 | 1990-09-18 | 1991-03-20 | $7700.00 | Window Closed / Lapsed |