US4144127A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for immobilizing glucose isomerase
Assignee: Unilever Bestfoods North America
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Filing: 1977-03-23
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Granted: 1979-03-13
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Expires: 1997-03-23
Abstract
Abstract Glucose isomerase is immobilized in an active form by adsorbing the glucose isomerase onto a colloidal silica. The enzyme is contacted with the colloidal silica and the resulting composite solidified by freezing. Optionally, the composite may be gelatinized prior to freezing. The composite is then used for the isomerization of glucose to fructose.
Inventors
- 👤 Shigehiro Enokizono
- 👤 Soichiro Ushiro
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 19:18
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; C12P; C12P19/00; C12P19/24; Y; Y10; Y10S; Y10S435/00; Y10S435/813
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
59 words
A process for immobilizing glucose isomerase comprising adsorbing glucose isomerase enzyme onto colloidal silica by contacting said enzyme with said colloidal silica in aqueous medium, solidifying the glucose isomerase adsorbed silica by freezing at a temperature of about -10° C. to about -30° C. and then thawing the resultant frozen composition to obtain an active immobilized glucose isomerase composite.
Claim 2
Dependent Claim
59 words
A process for immobilizing glucose isomerase comprising adsorbing glucose isomerase enzyme onto colloidal silica by contacting said enzyme with said colloidal silica in aqueous medium, solidifying the glucose isomerase adsorbed silica by freezing at a temperature of about -10° C. to about -30° C. and then thawing the resultant frozen composition to obtain an active immobilized glucose isomerase composite.
Claim 3
Dependent Claim
18 words
A process in accordance with claim 1, wherein the glucose isomerase adsorbed silica is gelatinized prior to freezing.
Claim 4
Dependent Claim
64 words
A process for immobilizing glucose isomerase comprising, adsorbing glucose isomerase enzyme onto colloidal silica by contacting said enzyme with said colloidal silica in aqueous medium, solidifying the glucose isomerase adsorbed silica by freezing and then thawing the resultant frozen composition to obtain an active immobilized glucose isomerase composite in the form of granules or flakes having a particle size of 20 to 100 mesh.
Claim 5
Dependent Claim
64 words
A process for immobilizing glucose isomerase comprising, adsorbing glucose isomerase enzyme onto colloidal silica by contacting said enzyme with said colloidal silica in aqueous medium, solidifying the glucose isomerase adsorbed silica by freezing and then thawing the resultant frozen composition to obtain an active immobilized glucose isomerase composite in the form of granules or flakes having a particle size of 20 to 100 mesh.
Claim 6
Dependent Claim
18 words
A process in accordance with claim 3, wherein the glucose isomerase adsorbed silica is gelatinized prior to freezing.
Claim 7
Dependent Claim
21 words
A process in accordance with claim 3, wherein the glucose isomerase adsorbed silica is treated with bifunctional reagent prior to freezing.
Claim 8
Dependent Claim
11 words
An immobilized active enzyme produced by the process of claim 5.
Claim 9
Dependent Claim
11 words
An immobilized active enzyme produced by the process of claim 3.
Claim 10
Dependent Claim
34 words
An enzyme composite in accordance with claim 7, wherein said composite has a particle size of 20 to 100 mesh and is composed of colloidal silica of about 10 to about 30mμ in diameter.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-03-12 | 1982-09-11 | 1983-03-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-03-12 | 1986-09-11 | 1987-03-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-03-12 | 1990-09-11 | 1991-03-13 | $7700.00 | Window Closed / Lapsed |