Patent US4143080A successfully refreshed from Google Patents.
US4143080A
Expired / Lapsed
US
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Method and reagent for the assay of hydroperoxide
Assignee: Goodyear Tire and Rubber Co
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Filing: 1977-11-11
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Granted: 1979-03-06
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Expires: 1997-11-11
Abstract
Abstract In a method for detection and/or assay of hydroperoxides (or, indirectly, of substances, such as cholesterol or glucose, which react to liberate hydroperoxides) using an assay reagent comprising a water-soluble iodide, a redox catalyst and a stabilizer, an improvement is provided wherein when the determination is made in the presence of protein, the redox catalyst concentration in the reagent is 5-500 μM and the stabilizer concentration in the reagent is 5-100 mg/l. A corresponding improvement in the assay reagent is also provided.
Inventors
- 👤 Hans-Dieter Harders
- 👤 Roland Helger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 15:53
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/60; C12Q1/26; C12Q1/28; C12Q1/54; Y; Y10; Y10S; Y10S436/00; Y10S436/904
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
74 words
In a process for the photometric determination of hydroperoxides per se in a protein-containing body fluid, or indirectly of substances therein which react to liberate hydroperoxides, using an assay reagent comprising a water-soluble iodide, a redox catalyst and stabilizer, the improvement wherein the concentration of the redox catalyst in the reagent is 5-500 μM, and the concentration of stabilizer is 5-100 mg/l whereby the body fluid does not require deproteinization before the photometric determination.
Claim 2
Dependent Claim
74 words
In a process for the photometric determination of hydroperoxides per se in a protein-containing body fluid, or indirectly of substances therein which react to liberate hydroperoxides, using an assay reagent comprising a water-soluble iodide, a redox catalyst and stabilizer, the improvement wherein the concentration of the redox catalyst in the reagent is 5-500 μM, and the concentration of stabilizer is 5-100 mg/l whereby the body fluid does not require deproteinization before the photometric determination.
Claim 3
Dependent Claim
15 words
The improvement of claim 1, wherein the redox catalyst is a molybdate, tungstate or vanadate.
Claim 4
Dependent Claim
15 words
The improvement of claim 2, wherein 5-50 μM of ammonium molybdate is the redox catalyst.
Claim 5
Dependent Claim
24 words
The improvement of claim 1, wherein sodium azide, hexamethylenetetramine, potassium fluoride, potassium thiocyanate, ethylenediaminetetraacetic acid, nitrilotriacetic acid or a mixture thereof is the stabilizer.
Claim 6
Dependent Claim
70 words
In a process for the photometric determination in a protein-containing body fluid of hydrogen peroxide liberated from cholesterol treated with cholesterol oxidase, using an assay reagent comprising a water-soluble iodide, a redox catalyst and stabilizer, the improvement wherein the concentration of the redox catalyst in the reagent is 5-500 μM, and the concentration of stabilizer is 5-100 mg/l whereby the body fluid does not require deproteinization before the photometric determination.
Claim 7
Dependent Claim
70 words
In a process for the photometric determination in a protein-containing body fluid of hydrogen peroxide liberated from cholesterol treated with cholesterol oxidase, using an assay reagent comprising a water-soluble iodide, a redox catalyst and stabilizer, the improvement wherein the concentration of the redox catalyst in the reagent is 5-500 μM, and the concentration of stabilizer is 5-100 mg/l whereby the body fluid does not require deproteinization before the photometric determination.
Claim 8
Dependent Claim
23 words
The improved process of claim 5, wherein the determination is carried out in the presence of polyethylene glycol mono-(1,1,3,3-tetramethylbutyl)-phenyl ether and alkyldimethylbenzylammonium chloride.
Claim 9
Dependent Claim
48 words
In a reagent for the photometric determination of hydroperoxides per se or indirectly of substances which react to liberate hydroperoxides, comprising a water-soluble iodide, a redox catalyst and stabilizer, the improvement wherein the concentration of redox catalyst is 5-500 μM and the concentration of stabilizer is 5-100 mg/l.
Claim 10
Dependent Claim
48 words
In a reagent for the photometric determination of hydroperoxides per se or indirectly of substances which react to liberate hydroperoxides, comprising a water-soluble iodide, a redox catalyst and stabilizer, the improvement wherein the concentration of redox catalyst is 5-500 μM and the concentration of stabilizer is 5-100 mg/l.
Claim 11
Dependent Claim
30 words
The improvement of claim 7, wherein the redox catalyst is 5-50 μM of ammonium molydbate, the stabilizer is 10 mg/l of sodium azide and the soluble iodide is potassium iodide.
Claim 12
Independent Claim
54 words
A method of detecting and assaying hydroperoxide in the presence of protein which comprises photometrically measuring the amount of triiodide ion formed when a reagent comprising water-soluble iodide, a redox catalyst in a concentration of 5-500 μM and stabilizer in a concentration of 5-100 mg/l is mixed with a protein- and hydroperoxide-containing test specimen.
Claim 13
Independent Claim
54 words
A method of detecting and assaying hydroperoxide in the presence of protein which comprises photometrically measuring the amount of triiodide ion formed when a reagent comprising water-soluble iodide, a redox catalyst in a concentration of 5-500 μM and stabilizer in a concentration of 5-100 mg/l is mixed with a protein- and hydroperoxide-containing test specimen.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-03-05 | 1982-09-04 | 1983-03-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-03-05 | 1986-09-04 | 1987-03-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-03-05 | 1990-09-04 | 1991-03-06 | $7700.00 | Window Closed / Lapsed |