US4138479A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the preparation of immunopotentiating agents from components of yeast cell wall material
Assignee: DONZIS LABORATORIES Inc A CORP
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Filing: 1977-09-12
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Granted: 1979-02-06
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Expires: 1997-09-12
Abstract
Abstract A water soluble immunopotentiating agent is derived from yeast cell wall material, including mechanically disrupted yeast cell walls, proteolyzed yeast cell wall material and carbohydrate-protein complexes found in yeast cell wall material, through extraction with a water-phenol mixture. The water soluble agent is isolated from the water phase and can be further purified, as through dialysis, to remover low molecular weight components.
Inventors
- 👤 Ernst Truscheit
- 👤 Robert Bierling
- 👤 Horst D. Schlumberger
- 👤 Herbert F. Oettgen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 19:47
CPC Classifications
C; C12; C12P; C12P21/00; C12P21/005; A; A61; A61K; A61K36/00; A61K36/06; A61K36/062; A61K36/064; C12N; C12N1/00; C12N1/14; C12N1/16; C12N1/165; C12N1/18; C12N1/185; C12P1/00; C12P1/02; C12P19/00; C12P19/04; C12R; C12R2001/00; C12R2001/645; C12R2001/72; C12R2001/85; C12R2001/865
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
49 words
The process for the preparation of a water soluble immunopotentiating agent from components of yeast cell walls which comprises subjecting yeast cell wall material, proteolyzed yeast cell wall material or a carbohydrate-protein yeast cell wall material to extraction with water and phenol and thereafter isolating the water soluble agent.
Claim 2
Dependent Claim
49 words
The process for the preparation of a water soluble immunopotentiating agent from components of yeast cell walls which comprises subjecting yeast cell wall material, proteolyzed yeast cell wall material or a carbohydrate-protein yeast cell wall material to extraction with water and phenol and thereafter isolating the water soluble agent.
Claim 3
Dependent Claim
41 words
The process according to claim 1 wherein any low molecular weight sugars, amino acids, peptides, lipids and yeast metabolites are removed from said aqueous phase by subjecting the same to gel filtration, ultrafiltration or dialysis prior to isolating said immunopotentiating agent.
Claim 4
Dependent Claim
32 words
The process according to claim 2 wherein said low molecular weight sugars, amino acids, peptides, lipids and yeast metabolites are removed by dialysis, said immunopotentiating material being obtained from the dialysis retentate.
Claim 5
Dependent Claim
22 words
The process according to claim 1 wherein said extraction is effected with a mixture of substantially equal parts of water and phenol.
Claim 6
Dependent Claim
27 words
The process according to claim 4 wherein the amount of said mixture is from about 10 to about 100 times the weight of the material being extracted.
Claim 7
Dependent Claim
70 words
The process according to claim 1 wherein mechanically disrupted yeast cell wall material is subjected to extraction with from 10 to 100 times the amount by volume of a mixture of substantially equal amounts of water and phenol, and the aqueous phase is separated and thereafter dialyzed to remove any low molecular weight sugars, amino acids, peptides, lipids and yeast metabolites, said immunopotentiating agent being isolated from the dialysis retentate.
Claim 8
Dependent Claim
13 words
The water soluble immunopotentiating agent produced according to the process of claim 6.
Claim 9
Dependent Claim
71 words
The process according to claim 1 wherein trypsin-proteolyzed yeast cell wall material is subjected to extraction with from 10 to 100 times the amount by volume of a mixture of substantially equal amounts by volume of water and phenol and the aqueous phase is separated and thereafter dialyzed to remove any low molecular weight sugars, amino acids, peptides, lipids and yeast metabolites, said immunopotentiating agent being isolated from the dialysis retentate.
Claim 10
Dependent Claim
13 words
The water soluble immunopotentiating agent produced according to the process of claim 8.
Claim 11
Dependent Claim
76 words
The process according to claim 1 wherein at least one of glucan-protein complex and a glucomannan-protein complex is sujected to extraction with from 10 to 100 times the amount by volume of a mixture of substantially equal amounts by volume of water and phenol and the aqueous phase is separated and thereafter dialyzed to remove any low molecular weight sugars, amino acids, peptides, lipids and yeast metabolites, said immunopotentiating agent being isolated from the dialysis retentate.
Claim 12
Dependent Claim
13 words
The water soluble immunopotentiating agent produced according to the process of claim 10.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-02-05 | 1982-08-07 | 1983-02-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-02-05 | 1986-08-07 | 1987-02-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-02-05 | 1990-08-07 | 1991-02-06 | $7700.00 | Window Closed / Lapsed |