US4138290A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Glucose isomerization under expanded bed conditions
Assignee: Novo Laboratories Inc
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Filing: 1976-04-22
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Granted: 1979-02-06
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Expires: 1996-04-22
Abstract
Abstract Expanded bed isomerization of glucose syrup to a glucose-fructose mixture having a fructose proportion exceeding about 40% of the glucose-fructose content by passing 30-55% w/w of glucose syrup through a cell-mass glucose isomerase preparation in the form of a bed of uniform particles of controlled size in the range of 150-2000 micrometers at a superficial syrup velocity of 0.5-15 meters an hour. Velocity and particle size are inversely related to create bed expansion within the range of 5-100%. Bed life may be extended by substituting fresh catalyst for inactivated catalyst. The isomerase should have an activity exceeding about 100 IGIC u/gm. The isomerase should be weighted by incorporation therein of finely divided alumina particles.
Inventors
- 👤 William H. McMullen
- 👤 William Carasik
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 19:58
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/04; C12P; C12P19/00; C12P19/24
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
121 words
A process utilizing expanded bed isomerization to convert glucose syrup to a glucose-fructose mixture containing at least 40% fructose by weight of the glucose-fructose content which comprises passing a 30-55% w/w of glucose syrup at isomerizing temperature and pH conditions up through at least one bed of a cell mass particulate form glucose isomerase preparation having uniform size in the range of 150-2,500 micrometers at a superficial linear velocity for the syrup of 0.5-15 meters an hour, said velocity and particle size being related in an inverse relation to create a bed expansion within the range of 5-100% and plug flow by the syrup through the bed, the bed depth being sufficient to achieve the aforementioned at least 40% fructose content.
Claim 2
Dependent Claim
121 words
A process utilizing expanded bed isomerization to convert glucose syrup to a glucose-fructose mixture containing at least 40% fructose by weight of the glucose-fructose content which comprises passing a 30-55% w/w of glucose syrup at isomerizing temperature and pH conditions up through at least one bed of a cell mass particulate form glucose isomerase preparation having uniform size in the range of 150-2,500 micrometers at a superficial linear velocity for the syrup of 0.5-15 meters an hour, said velocity and particle size being related in an inverse relation to create a bed expansion within the range of 5-100% and plug flow by the syrup through the bed, the bed depth being sufficient to achieve the aforementioned at least 40% fructose content.
Claim 3
Dependent Claim
20 words
The process of claim 1 wherein the life of the bed is extended by intermittently or continuously adding fresh enzyme.
Claim 4
Dependent Claim
18 words
The process of claim 1 wherein the activity of the glucose isomerase preparation exceeds about 100 IGIC u/gram.
Claim 5
Dependent Claim
40 words
The process of claim 1 wherein isomerization is carried out until the unit activity of the enzyme has declined to about 25% of its initial activity at an ever decreasing syrup flow, the flow rate decreasing as enzyme activity decreases.
Claim 6
Dependent Claim
28 words
The process of claim 1 wherein the cell mass particulate form glucose isomerase preparation contains uniformly dispersed therein finely divided aluminum oxide as from 5-20% by weight thereof.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-02-05 | 1982-08-07 | 1983-02-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-02-05 | 1986-08-07 | 1987-02-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-02-05 | 1990-08-07 | 1991-02-06 | $7700.00 | Window Closed / Lapsed |