Patent US4137225A successfully refreshed from Google Patents.
US4137225A
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Novel chromogenic enzyme substrates
Assignee: Chromogenix AB
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Filing: 1978-03-20
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Granted: 1979-01-30
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Expires: 1998-03-20
Abstract
Abstract Diagnostically active chromogenic substrate with good stability and high specificity to serine proteases having the formula: H-D-A 1 -A 2 -A 3 -NH-R or salts thereof, wherein A 1 is Gly, Ala, Val, Leu, Ile, Pip, Pro, or Aze; A 2 is Gly, Ala, Val, Leu, Ile, Pip, Pro, Aze, or Phe; A 3 is Arg, Lys, or Orn, and R is a chromophoric group.
Inventors
- 👤 Bo T. Af Ekenstam
- 👤 Leif E. Aurell
- 👤 Karl G. Claeson
- 👤 Birgitta G. Karlsson
- 👤 Stig I. Gustavsson
- 👤 Gun A. Olausson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 09:55
CPC Classifications
C; C07; C07K; C07K5/00; C07K5/04; C07K5/08; C07K5/0802; C07K5/0804; C07K5/0808; C12; C12Q; C12Q2337/00; C12Q2337/10; C12Q2337/12; Y; Y10; Y10S; Y10S530/00; Y10S530/802
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
93 words
Diagnostically active chromogenic substrate with a good solubility and a high specificity to serine proteases, having the following general formula: H-D-A.sub.1 -A.sub.2 -A.sub.3 -NH-R or salts thereof, wherein A 1 is an amino acid selected from the group consisting of Gly, Ala, Val, Leu, Ile, Pip, Pro, and Aze; A 2 is an amino acid selected from the group consisting of Gly, Ala, Val, Leu, Ile, Pip, Pro, Aze, and Phe; A 3 is an amino acid selected from the group consisting of Arg, Lys, and Orn; and R is a chromophoric group.
Claim 2
Dependent Claim
93 words
Diagnostically active chromogenic substrate with a good solubility and a high specificity to serine proteases, having the following general formula: H-D-A.sub.1 -A.sub.2 -A.sub.3 -NH-R or salts thereof, wherein A 1 is an amino acid selected from the group consisting of Gly, Ala, Val, Leu, Ile, Pip, Pro, and Aze; A 2 is an amino acid selected from the group consisting of Gly, Ala, Val, Leu, Ile, Pip, Pro, Aze, and Phe; A 3 is an amino acid selected from the group consisting of Arg, Lys, and Orn; and R is a chromophoric group.
Claim 3
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Val-Pro-Arg-pNA, or salt thereof.
Claim 4
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Val-Pip-Arg-pNA, or salt thereof.
Claim 5
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Val-Leu-Arg-pNA, or salt thereof.
Claim 6
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Val-Leu-Lys-pNA, or salt thereof.
Claim 7
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Ile-Leu-Arg-pNA, or salt thereof.
Claim 8
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Ile-Leu-Lys-pNA, or salt thereof.
Claim 9
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Pro-Phe-Arg-pNA, or salt thereof.
Claim 10
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Val-Pip-Arg-pNA, or salt thereof.
Claim 11
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Pro-Phe-Lys-pNA, or salt thereof.
Claim 12
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Ala-Ala-Arg-pNA, or salt thereof.
Claim 13
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Leu-Gly-Arg-pNA, or salt thereof.
Claim 14
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Leu-Ile-Arg-pNA, or salt thereof.
Claim 15
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Leu-Val-Arg-pNA, or salt thereof.
Claim 16
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Leu-Val-Lys-pNA, or salt thereof.
Claim 17
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Pip-Phe-Arg-pNA, or salt thereof.
Claim 18
Dependent Claim
11 words
The substrate of claim 1 which is H-D-Leu-Leu-Arg-pNA, or salt thereof.
Claim 19
Dependent Claim
10 words
The substrate of claim 1 wherein A 1 is Val.
Claim 20
Dependent Claim
10 words
The substrate of claim 1 wherein A 1 is Ile.
Claim 21
Dependent Claim
10 words
The substrate of claim 1 wherein A 1 is Pro.
Claim 22
Dependent Claim
10 words
The substrate of claim 1 wherein A 2 is Pro.
Claim 23
Dependent Claim
10 words
The substrate of claim 1 wherein A 2 is Pip.
Claim 24
Dependent Claim
10 words
The substrate of claim 1 wherein A 2 is Leu.
Claim 25
Dependent Claim
10 words
The substrate of claim 1 wherein A 2 is Phe.
Claim 26
Dependent Claim
10 words
The substrate of claim 1 wherein A 3 is Arg.
Claim 27
Dependent Claim
10 words
The substrate of claim 1 wherein A 3 is Lys.
Claim 28
Dependent Claim
10 words
The substrate of claim 1 which is a hydrochloride salt.
Claim 29
Dependent Claim
19 words
The substrate of claim 1 wherein R is nitrophenyl or naphthyl or nitronaphthyl or methoxynaphthyl or quinolyl or nitroquinolyl.
Claim 30
Dependent Claim
9 words
The substrate of claim 1 wherein R is nitrophenyl.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-01-29 | 1982-07-31 | 1983-01-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-01-29 | 1986-07-31 | 1987-01-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-01-29 | 1990-07-31 | 1991-01-30 | $7700.00 | Window Closed / Lapsed |