US4130461A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Continuous process for the production of polysaccharide under phosphate limiting conditions
Assignee: Tate and Lyle PLC
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Filing: 1977-05-20
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Granted: 1978-12-19
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Expires: 1997-05-20
Abstract
Abstract Polysaccharide is produced in high yields by continuous culture of a polysaccharide-producing strain of Azotobacter vinelandii under phosphate-limited conditions, preferably with restricted oxygen uptake.
Inventors
- 👤 Renton C. Righelato
- 👤 Lynda Deavin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 07:38
CPC Classifications
C; C08; C08B; C08B37/00; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12P; C12P19/00; C12P19/04; C12R; C12R2001/00; C12R2001/01; C12R2001/065; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/831
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
131 words
In a process for the production of a polysaccharide comprising subjecting to continuous cultivation a bacterium of the species Azotobacter vinelandii under aerobic conditions to allow an oxygen uptake in an aqueous culture medium containing as essential ingredients at least one monosaccharide or disaccharide as carbon source and sources of phosphate, molybdenum, iron, magnesium, potassium, sodium, calcium and sulphate, said medium being provided with a source of nitrogen selected from the group consisting of a fixed nitrogen source and gaseous nitrogen, and recovering the resulting polysaccharide the improvement which comprises maintaining the concentration of phosphate in the medium such that it limits the concentration of the bacteria and is at least 1.0 millimolar, and maintaining the pH of the medium during cultivation within the range of about 6.0 to about 8.2.
Claim 2
Dependent Claim
131 words
In a process for the production of a polysaccharide comprising subjecting to continuous cultivation a bacterium of the species Azotobacter vinelandii under aerobic conditions to allow an oxygen uptake in an aqueous culture medium containing as essential ingredients at least one monosaccharide or disaccharide as carbon source and sources of phosphate, molybdenum, iron, magnesium, potassium, sodium, calcium and sulphate, said medium being provided with a source of nitrogen selected from the group consisting of a fixed nitrogen source and gaseous nitrogen, and recovering the resulting polysaccharide the improvement which comprises maintaining the concentration of phosphate in the medium such that it limits the concentration of the bacteria and is at least 1.0 millimolar, and maintaining the pH of the medium during cultivation within the range of about 6.0 to about 8.2.
Claim 3
Dependent Claim
27 words
The process of claim 1, in which the strain of A vinelandii is selected from the group consisting of strains NCIB 9068 NCIB 8789 and NCIB 8660.
Claim 4
Dependent Claim
22 words
The process of claim 1, in which the concentration of phosphate in the medium is from about 2.0 to about 3.0 millimolar.
Claim 5
Dependent Claim
24 words
The process of claim 1, in which the oxygen uptake is restricted to a level giving optimum conversion of monosaccharide or disaccharide into polysaccharide.
Claim 6
Dependent Claim
23 words
The process of claim 4, in which the oxygen uptake is restricted to a level of about 7 to about 22 millimoles/g cell/hour.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-12-18 | 1982-06-19 | 1982-12-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-12-18 | 1986-06-19 | 1986-12-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-12-18 | 1990-06-19 | 1990-12-19 | $7700.00 | Window Closed / Lapsed |