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US4127448A
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Amperometric-non-enzymatic method of determining sugars and other polyhydroxy compounds
Assignee: FIRST WISCONSIN NATIONAL BANK OF MILWAUKEE
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Filing: 1977-02-28
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Granted: 1978-11-28
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Expires: 1997-02-28
Abstract
Abstract A method for determining hydrogen peroxide, sugars and other polyhydroxy compounds in blood serum, urine and aqueous solutions by direct electrochemical oxidation in aqueous alkaline medium with a transition metal oxide catalyst electrode in the presence of lithium hydroxide in solution.
Inventors
- 👤 Karl G. Schick
- 👤 Calvin O. Huber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 23:18
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/327; G01N27/3271; G01N27/3273
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
67 words
A method for determination of hydrogen peroxide, sugars and other polyhydroxy compounds comprising providing the compound in an aqueous alkaline solution, applying a constant potential between a transition metal oxide catalyst electrode and a counter electrode immersed in the solution whereby electrocatalytic oxidation of the hydroxy compound occurs at the surface of the transition metal oxide electrode, and measuring the rise in current from the electrochemical oxidation.
Claim 2
Independent Claim
67 words
A method for determination of hydrogen peroxide, sugars and other polyhydroxy compounds comprising providing the compound in an aqueous alkaline solution, applying a constant potential between a transition metal oxide catalyst electrode and a counter electrode immersed in the solution whereby electrocatalytic oxidation of the hydroxy compound occurs at the surface of the transition metal oxide electrode, and measuring the rise in current from the electrochemical oxidation.
Claim 3
Dependent Claim
21 words
The method as claimed in claim 1 in which the solution has a pH within the range of 10 to 14.
Claim 4
Dependent Claim
20 words
The method as claimed in claim 1 in which lithium hydroxide is present in the dissolved state in the solution.
Claim 5
Dependent Claim
22 words
The method as claimed in claim 1 in which the solution contains ions of the transition metal of the oxide catalyst electrode.
Claim 6
Dependent Claim
31 words
The method as claimed in claim 1 in which the oxide electrode is covered with a microporous membrane to keep proteins and high molecular weight molecules from interfering with the determination.
Claim 7
Dependent Claim
34 words
The method as claimed in claim 1 in which the solution contains the material to be determined in a concentration within the range of 1 × 10 -7 to 1 × 10 -2 M.
Claim 8
Dependent Claim
56 words
The method as claimed in claim 1 in which, when the polyhydroxy compound is serum glucose, the oxide electrode is protected with a microporous membrane and the solution has a pH within the range of 11 to 13, with lithium hydroxide and ions of the metal of the electrode present in low concentration in the solution.
Claim 9
Dependent Claim
32 words
The method as claimed in claim 1, in which, in the determination of serum triglyceride, the polyhydroxy compound is glycerol derived by hydrolyzation of the serum triglyceride to glycerol and fatty acid.
Claim 10
Dependent Claim
25 words
The method as claimed in claim 8 in which the hydrolyzation is carried out by reacting the serum triglyceride with the enzymes lipase and α-chymotrypsin.
Claim 11
Dependent Claim
16 words
The method as claimed in claim 9 in which the reaction is carried out by incubation.
Claim 12
Dependent Claim
51 words
The method as claimed in claim 1 in which, in the determination of cholesterol in blood, the polyhydroxy compound is hydrogen peroxide derived by reacting the serum cholesterol with cholesterol ester hydrolase to convert cholesterol esters to free cholesterol and reacting the free cholesterol with cholesterol oxidase to yield hydrogen peroxide.
Claim 13
Dependent Claim
29 words
The method as claimed in claim 1 in which, in the determination of sugar in urine, the urine is first reacted with urease and uricase to deactivate uric acid.
Claim 14
Dependent Claim
43 words
The method as claimed in claim 12 in which the determination of sugar in urine is made both before and after reaction of the urine with urease and uricase whereby the difference in current levels indicates the amount of sugar in the urine.
Claim 15
Dependent Claim
31 words
The method as claimed in claim 1 in which the transition metal oxide is the oxide of the metal selected from the group consisting of manganese, copper, nickel, cobalt and siver.
Claim 16
Dependent Claim
26 words
The method as claimed in claim 1 in which the current rise is compared with the current rise of a known standard solution of hydroxy compound.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-11-27 | 1982-05-29 | 1982-11-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-11-27 | 1986-05-29 | 1986-11-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-11-27 | 1990-05-29 | 1990-11-28 | $7700.00 | Window Closed / Lapsed |