Patent US4120945A successfully refreshed from Google Patents.
US4120945A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Substrate coated with receptor and labeled ligand for assays
Assignee: Becton Dickinson and Co
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Filing: 1976-07-06
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Granted: 1978-10-17
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Expires: 1996-07-06
Abstract
Abstract Solid substrate is coated with a receptor, with a limited portion of the receptor being reversibly coated with a labeled form of a ligand to be assayed, whereby during an assay procedure the labeled form of the ligand is free to re-equilibrate with the receptor in competition with the ligand to be assayed.
Inventors
- 👤 Sidney Gutcho
- 👤 Henry McCarter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 08:25
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N2470/00; G01N2470/10; G01N2470/12; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
91 words
An article for the assay of a ligand, comprising: a solid, a receptor for both the ligand to be assayed and a labeled form of the ligand to be assayed on said solid, and a labeled form of the ligand to be assayed reversibly coated on at least 0.5% and no more than 25% of the surface of said receptor, whereby during an assay for the ligand, the labeled form of the ligand is removed from the receptor for re-equilibration with the receptor in competition with the ligand to be assayed.
Claim 2
Dependent Claim
91 words
An article for the assay of a ligand, comprising: a solid, a receptor for both the ligand to be assayed and a labeled form of the ligand to be assayed on said solid, and a labeled form of the ligand to be assayed reversibly coated on at least 0.5% and no more than 25% of the surface of said receptor, whereby during an assay for the ligand, the labeled form of the ligand is removed from the receptor for re-equilibration with the receptor in competition with the ligand to be assayed.
Claim 3
Dependent Claim
27 words
The article of claim 1 wherein the receptor on the solid was applied in the presence of a blocking agent for the receptor and a deaggregating agent.
Claim 4
Dependent Claim
26 words
The article of claim 1 wherein the labeled form of the ligand to be assayed is reversibly coated on at least 0.5% of the receptor surface.
Claim 5
Dependent Claim
24 words
The article of claim 3 wherein the labeled form of the ligand is reversibly coated on no more than 25% of the receptor surface.
Claim 6
Dependent Claim
18 words
The article of claim 1 wherein the labeled form of the ligand is labeled with a radioactive isotope.
Claim 7
Dependent Claim
11 words
The article of claim 5 wherein the solid is a plastic.
Claim 8
Dependent Claim
33 words
The article of claim 6 wherein the solid plastic is in the form of a tube and only the bottom portion of the receptor on the tube has the labeled ligand coated thereon.
Claim 9
Dependent Claim
11 words
The article of claim 5 wherein the receptor is an antibody.
Claim 10
Dependent Claim
26 words
The article of claim 8 wherein the ligand to be assayed is digoxin and the labeled form of the ligand is a radiolabeled form of digoxigenin.
Claim 11
Dependent Claim
10 words
The article of claim 9 wherein the plastic is polystyrene.
Claim 12
Dependent Claim
19 words
The article of claim 10 wherein the labeled form of the ligand is 3-0-succinyl digoxigenin tyrosine [ 125 I].
Claim 13
Dependent Claim
79 words
In a process for the assay of a ligand, the improvement comprising: equilibrating a sample including the ligand to be assayed with a solid phase having a receptor applied thereto and a labeled form of the ligand reversibly coated on at least 0.5% and no more than 25% of the surface of the receptor, whereby the labeled form of the ligand is removed from the receptor for re-equilibration with the receptor in competition with the ligand to be assayed.
Claim 14
Dependent Claim
79 words
In a process for the assay of a ligand, the improvement comprising: equilibrating a sample including the ligand to be assayed with a solid phase having a receptor applied thereto and a labeled form of the ligand reversibly coated on at least 0.5% and no more than 25% of the surface of the receptor, whereby the labeled form of the ligand is removed from the receptor for re-equilibration with the receptor in competition with the ligand to be assayed.
Claim 15
Dependent Claim
11 words
The proess of claim 12 wherein the assay is a radioimmunoassay.
Claim 16
Dependent Claim
17 words
The process of claim 12 wherein the solid phase is in the form of a plastic tube.
Claim 17
Dependent Claim
31 words
The process of claim 12 wherein the labeled form of the ligand to be assayed is reversibly coated on only the bottom portion of the receptor surface on the plastic tube.
Claim 18
Dependent Claim
89 words
In a process for preparing an article for the assay of a ligand wherein a receptor for the ligand to be assayed is coated on a tube, the improvement comprising: coating the labeled form of the ligand to be assayed on only the bottom portion of the receptor on the tube whereby the labeled form of the ligand is reversibly coated on at least 0.5% and no more than 25% of the surface of the receptor for re-equilibration with the receptor in competition with the ligand to be assayed.
Claim 19
Dependent Claim
89 words
In a process for preparing an article for the assay of a ligand wherein a receptor for the ligand to be assayed is coated on a tube, the improvement comprising: coating the labeled form of the ligand to be assayed on only the bottom portion of the receptor on the tube whereby the labeled form of the ligand is reversibly coated on at least 0.5% and no more than 25% of the surface of the receptor for re-equilibration with the receptor in competition with the ligand to be assayed.
Claim 20
Dependent Claim
13 words
The process of claim 16 wherein the coating is effected at room temperature.
Claim 21
Dependent Claim
14 words
The process of claim 17 wherein the ligand is labeled with a radioactive isotope.
Claim 22
Dependent Claim
11 words
The process of claim 18 wherein the receptor is an antibody.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-10-16 | 1982-04-17 | 1982-10-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-10-16 | 1986-04-17 | 1986-10-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-10-16 | 1990-04-17 | 1990-10-17 | $7700.00 | Window Closed / Lapsed |