Patent US4118349A successfully refreshed from Google Patents.
US4118349A
Expired / Lapsed
US
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Process for the manufacture of polystyrene latex compounds
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1977-06-30
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Granted: 1978-10-03
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Expires: 1997-06-30
Abstract
Abstract The present invention relates to a process for the manufacture of protein or peptide polystyrene latex compounds useful as diagnostic agents, in which a protein or peptide is linked by means of an aromatic diazonium compound to a homo- or copolymer of styrene as well as the compounds obtained by said process.
Inventors
- 👤 Ludwig Bonacker
- 👤 Josef Stark
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 21:57
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; C; C08; C08F; C08F8/00; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/947; Y10S436/00; Y10S436/823
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
100 words
In a process for chemically bonding a protein or peptide to a polymeric carrier which is a styrene homopolymer or a copolymer of styrene with less than 50 percent by weight of acrylic acid or methacrylic acid by contacting (1) a homogeneous aqueous latex suspension of said polymeric carrier, (2) said protein or peptide, and (3) an aromatic diazonium compound at a weakly alkaline pH, the improvements which comprise contacting (1), (2) and (3) at a temperature below room temperature and subsequently adding albumin to the mixture to give an albumin concentration between 0.5 and 1.5 percent in the mixture.
Claim 2
Dependent Claim
100 words
In a process for chemically bonding a protein or peptide to a polymeric carrier which is a styrene homopolymer or a copolymer of styrene with less than 50 percent by weight of acrylic acid or methacrylic acid by contacting (1) a homogeneous aqueous latex suspension of said polymeric carrier, (2) said protein or peptide, and (3) an aromatic diazonium compound at a weakly alkaline pH, the improvements which comprise contacting (1), (2) and (3) at a temperature below room temperature and subsequently adding albumin to the mixture to give an albumin concentration between 0.5 and 1.5 percent in the mixture.
Claim 3
Dependent Claim
24 words
A method as in claim 1, wherein said latex and diazonium compound are first combined and said protein or peptide is then combined therewith.
Claim 4
Dependent Claim
24 words
A method as in claim 1, wherein said latex and protein or peptide are first combined and said diazonium compound is then combined therewith.
Claim 5
Dependent Claim
36 words
A method in claim 1, wherein said diazonium compound is a diazotized amine selected from the group consisting of 2-naphthylamine; 7-ethoxy-3,9-diamino-acridine; aniline; substituted anilines; phenylene diamine, benzidine; dianisidine; diamino-diphenylmethane; diamino-diphenyl-sulfone; benzidine sulfonic acids and p-amino-phenylsulfone-ethoxy-sulfonic acids.
Claim 6
Dependent Claim
24 words
A method as in claim 1, wherein said protein or peptide is a plasmaprotein; a proteohormone; or a viral, bacterial, protozoic, or helminthic protein.
Claim 7
Dependent Claim
18 words
A method as in claim 1, wherein said protein or peptide is an IgE-globulin or the corresponding antibody.
Claim 8
Dependent Claim
17 words
A method as in claim 1, wherein said protein or peptide is the antibody corresponding to haptoglobin.
Claim 9
Dependent Claim
17 words
A method as in claim 1, wherein said protein or peptide is the antibody corresponding to α1-antitrypsin.
Claim 10
Dependent Claim
36 words
A method as in claim 1, wherein said protein or peptide is PP 1 ,PP 2 , or PP 3 pregnancy proteins, or the antibody corresponding to PP 1 , PP 2 or PP 3 .
Claim 11
Dependent Claim
17 words
A method as in claim 1, wherein said protein or peptide is the antibody corresponding to transaminase.
Claim 12
Dependent Claim
20 words
A method as in claim 1, wherein said protein or peptide is a rubella virus protein or the corresponding antibody.
Claim 13
Dependent Claim
14 words
A method as in claim 1, wherein said protein or peptide is a staphylolysin.
Claim 14
Dependent Claim
19 words
A method as in claim 1, wherein said protein or peptide is a bilharziasis antigen or the corresponding antibody.
Claim 15
Dependent Claim
18 words
A method as in claim 13, wherein said bilharziasis antigen or the antibody is derived from Schistosoma mansoni.
Claim 16
Dependent Claim
19 words
A method as in claim 1 wherein said protein or peptide is a protein fraction derived from Entameba histolytica.
Claim 17
Dependent Claim
17 words
A polymeric carrier having a protein or peptide bonded thereto, prepared by the method of claim 1.
Claim 18
Dependent Claim
17 words
A polymeric carrier having a protein or peptide bonded thereto, prepared by the method of claim 7.
Claim 19
Dependent Claim
17 words
A polymeric carrier having a protein or peptide bonded thereto, prepared by the method of claim 8.
Claim 20
Dependent Claim
27 words
A polymeric carrier having a protein or peptide bonded thereto, prepared by the method of claim 11, wherein said protein or peptide is a rubella virus protein.
Claim 21
Dependent Claim
17 words
A polymeric carrier having a protein or peptide bonded thereto, prepared by the method of claim 12.
Claim 22
Dependent Claim
26 words
A polymeric carrier having a protein or peptide bonded thereto, prepared by the method of claim 13, wherein said protein or peptide is a bilharziasis antigen.
Claim 23
Dependent Claim
18 words
A polymeric carrier having a protein or a peptide bonded thereto, prepared by the method of claim 15.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-10-02 | 1982-04-03 | 1982-10-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-10-02 | 1986-04-03 | 1986-10-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-10-02 | 1990-04-03 | 1990-10-03 | $7700.00 | Window Closed / Lapsed |