Patent US4116776A successfully refreshed from Google Patents.
US4116776A
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Diagnostic blood test and kit for detecting human chorionic gonadotropin
Assignee: IMMUNOPATHOLOGY INSTITUTE Inc
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Filing: 1976-04-19
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Granted: 1978-09-26
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Expires: 1996-04-19
Abstract
Abstract Malignant neoplastic cells synthesize and secrete human chorionic gonadotropin into the circulatory system, where the glycoprotein hormone is collected or adsorbed by the host's lymphocytes. This application discloses a clinical diagnostic test and kit for determining the presence of human chorionic gonadotropin in blood. Where the test is positive in a man or a non-pregnant woman cancer is present in the host.
Inventors
- 👤 Milton H. Dalbow
- 👤 Hernan F. Acevedo
- 👤 Malcolm Slifkin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 23:28
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/76; G01N2333/00; G01N2333/435; G01N2333/575; G01N2333/59; G01N2333/90; G01N2333/914; G01N2333/924; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S435/968; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/815; Y10S436/817; Y10S436/818
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
96 words
A diagnostic blood test for human chorionic gonadotropin comprising the steps of: (a) separating the white blood cells from the whole blood sample to be tested; (b) treating the cells with neuraminidase; (c) washing the cells to remove excess neuraminidase; (d) treating the cells with antiserum specific to the beta-subunit of human chorionic gonadotropin, said antiserum including a clinically detectable label; (e) washing excess antiserum from the cells; and (f) clinically determining the presence or absence of said label; the presence of said label indicating the presence of human chorionic gonadotropin in the blood sample tested.
Claim 2
Dependent Claim
96 words
A diagnostic blood test for human chorionic gonadotropin comprising the steps of: (a) separating the white blood cells from the whole blood sample to be tested; (b) treating the cells with neuraminidase; (c) washing the cells to remove excess neuraminidase; (d) treating the cells with antiserum specific to the beta-subunit of human chorionic gonadotropin, said antiserum including a clinically detectable label; (e) washing excess antiserum from the cells; and (f) clinically determining the presence or absence of said label; the presence of said label indicating the presence of human chorionic gonadotropin in the blood sample tested.
Claim 3
Dependent Claim
13 words
The diagnostic test defined in claim 1, wherein neuraminidase is vibro cholerera neuraminidase.
Claim 4
Dependent Claim
42 words
The diagnostic blood test defined in claim 1, wherein said label is a radioactive isotope and the presence of the label is determined by counting the radiation of the sample with a radiation counter, providing a quantitative analysis for human chorionic gonadotropin.
Claim 5
Dependent Claim
25 words
The diagnostic blood test defined in claim 3, wherein said radioactive isotope is selected from the group consisting of radioiodine 125, radioiodine 131 and tritium.
Claim 6
Dependent Claim
16 words
The diagnostic blood test defined in claim 3, wherein said label is conjugated to said antiserum.
Claim 7
Dependent Claim
22 words
The diagnostic blood test defined in claim 3, wherein said label is conjugated to a second antiserum specific to said first antiserum.
Claim 8
Dependent Claim
33 words
The diagnostic blood test defined in claim 1, wherein said label is fluorescein and the presence of said label and human chorionic gonadotropin is determined by examining said cells under a fluorescent microscope.
Claim 9
Dependent Claim
23 words
The diagnostic blood test defined in claim 7, wherein said fluorescein label in conjugated to a second antiserum specific to said first antiserum.
Claim 10
Dependent Claim
123 words
A diagnostic test for determining the presence of human chorionic gonadotropin in a blood sample, the presence of human chorionic gonadotropin indicating the presence of cancer, comprising the steps of: (a) separating the lymphocytes from the blood sample to be tested; (b) treating said lymphocyte cells with a neuraminidase; (c) washing said lymphocyte cells to remove excess neuraminidase; (d) treating said cells with an antiserum specific to the beta-subunit of human chorionic gonadotropin, said antiserum including a radioactive label; (e) washing said lymphocyte cells to remove excess antiserum; and (f) determining the presence of human chorionic gonadotropin in the lymphocyte cells by counting the gamma radiation; the presence of human chorionic gonadotropin indicating the presence of cancer where the host is not pregnant.
Claim 11
Dependent Claim
123 words
A diagnostic test for determining the presence of human chorionic gonadotropin in a blood sample, the presence of human chorionic gonadotropin indicating the presence of cancer, comprising the steps of: (a) separating the lymphocytes from the blood sample to be tested; (b) treating said lymphocyte cells with a neuraminidase; (c) washing said lymphocyte cells to remove excess neuraminidase; (d) treating said cells with an antiserum specific to the beta-subunit of human chorionic gonadotropin, said antiserum including a radioactive label; (e) washing said lymphocyte cells to remove excess antiserum; and (f) determining the presence of human chorionic gonadotropin in the lymphocyte cells by counting the gamma radiation; the presence of human chorionic gonadotropin indicating the presence of cancer where the host is not pregnant.
Claim 12
Dependent Claim
34 words
The diagnostic test defined in claim 9 wherein said neuraminidase is vibro cholerera neuraminidase and the concentration of vibro cholerera neuraminidase is about 10 units of vibro cholerera neuraminidase per 10 6 lymphocyte cells.
Claim 13
Dependent Claim
24 words
The diagnostic test defined in claim 9, wherein said radioactive label is selected from the group consisting of radioiodine 125, radioiodine 131 and tritium.
Claim 14
Dependent Claim
24 words
The diagnostic test defined in claim 8, wherein said radioactive label is conjugated to said antiserum specific to the beta-subunit of human chorionic gonadotropin.
Claim 15
Dependent Claim
131 words
A diagnostic blood test for determining the presence of human chorionic gonadotropin as an indication of cancer, comprising the steps of: (a) separating the white blood cells from the whole blood sample; (b) treating said cells with neuraminidase; (c) washing excess neuraminidase from said white blood cells; (d) treating said white blood cells with a first antiserum specific to the beta-subunit of human chorionic gonadotropin raised in a host animal; (e) treating said cells with a second antiserum specific to the gamma globulin of said host animal, conjugated to fluorescein; (f) washing excess antiserum from said white blood cells; and (g) examining said cells for fluorescence; if fluorescence is found, human chorionic gonadotropin is present in the blood sample tested and if the patient is not pregnant, cancer has been established.
Claim 16
Dependent Claim
131 words
A diagnostic blood test for determining the presence of human chorionic gonadotropin as an indication of cancer, comprising the steps of: (a) separating the white blood cells from the whole blood sample; (b) treating said cells with neuraminidase; (c) washing excess neuraminidase from said white blood cells; (d) treating said white blood cells with a first antiserum specific to the beta-subunit of human chorionic gonadotropin raised in a host animal; (e) treating said cells with a second antiserum specific to the gamma globulin of said host animal, conjugated to fluorescein; (f) washing excess antiserum from said white blood cells; and (g) examining said cells for fluorescence; if fluorescence is found, human chorionic gonadotropin is present in the blood sample tested and if the patient is not pregnant, cancer has been established.
Claim 17
Dependent Claim
27 words
The diagnostic test defined in claim 13, wherein said neuraminidase is vibro cholerera neuraminidase and the concentration is about 10 units of neuraminidase to 10 6 cells.
Claim 18
Dependent Claim
37 words
A diagnostic test kit for determining the presence of human chorionic gonadotropin in a blood sample, comprising a neuraminidase and an antiserum specific to the beta-subunit of human chorionic gonadotropin, said antiserum including a clinically detectable label.
Claim 19
Dependent Claim
37 words
A diagnostic test kit for determining the presence of human chorionic gonadotropin in a blood sample, comprising a neuraminidase and an antiserum specific to the beta-subunit of human chorionic gonadotropin, said antiserum including a clinically detectable label.
Claim 20
Dependent Claim
15 words
The diagnostic test kit defined in claim 15 wherein said neuraminidase is vibro cholerera neuraminidase.
Claim 21
Dependent Claim
27 words
The diagnostic test kit defined in claim 15, wherein said label is a radioactive label selected from the group consisting of radioiodine 125, radioiodine 131, and tritium.
Claim 22
Dependent Claim
13 words
The diagnostic test kit defined in claim 15, wherein said label is fluorescein.
Claim 23
Dependent Claim
47 words
The diagnostic test kit defined in claim 15, wherein said antiserum includes two separate constituents, including an antiserum specific to the beta-subunit of human chorionic gonadotropin raised in a host animal and an antiserum specific to the gamma globulin of said host animal conjugated to said label.
References Cited (Backward Citations)
US3791932A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-09-25 | 1982-03-27 | 1982-09-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-09-25 | 1986-03-27 | 1986-09-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-09-25 | 1990-03-27 | 1990-09-26 | $7700.00 | Window Closed / Lapsed |