US4113568A
Expired / Lapsed
US
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Process for the renewal of an insolubilized glucose isomerase
Assignee: Mitsubishi Chemical Corp
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Filing: 1977-03-24
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Granted: 1978-09-12
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Expires: 1997-03-24
Abstract
Abstract There is disclosed a process for the renewal of a deactivated insolubilized glucose isomerase which comprises subjecting an insolubilized glucose isomerase adsorbed onto an anion exchange resin, which has been used for isomerization of glucose into fructose to decrease its activity, to treatments by an aqueous mineral acid solution in combination with an aqueous alkaline solution, an aqueous electrolytic salt solution, an aqueous mineral acid or a mixture thereof to release adsorbed materials from the resin which is converted into a salt type and adsorbing fresh glucose isomerase to the regenerated resin to recover the activity of the insolubilized glucose isomerase.
Inventors
- 👤 Yoshimasa Fujita
- 👤 Akiyoshi Matsumoto
- 👤 Isao Miyachi
- 👤 Nobuo Imai
- 👤 Isao Kawakami
- 👤 Tadashi Hishida
- 👤 Akira Kamata
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 19:32
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/24; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
105 words
A process for the renewal of an insolubilized glucose isomerase comprising the steps of: (1) desorbing the adsorbed materials from an insolubilized glucose isomerase by treating sequentially with an aqueous mineral acid in combination with an aqueous alkali solution, an aqueous electrolytic salt solution, an aqueous mineral acid or a mixture thereof, said insolubilized isomerase being glucose isomerase adsorbed on and bonded to a supporter of synthetic anion exchange macroporous resin and having been used in the isomerization of glucose into fructose, (2) converting said supporter resin into a salt type to regenerate the supporter, and (3) adsorbing fresh glucose isomerase on the regenerated supporter.
Claim 2
Dependent Claim
105 words
A process for the renewal of an insolubilized glucose isomerase comprising the steps of: (1) desorbing the adsorbed materials from an insolubilized glucose isomerase by treating sequentially with an aqueous mineral acid in combination with an aqueous alkali solution, an aqueous electrolytic salt solution, an aqueous mineral acid or a mixture thereof, said insolubilized isomerase being glucose isomerase adsorbed on and bonded to a supporter of synthetic anion exchange macroporous resin and having been used in the isomerization of glucose into fructose, (2) converting said supporter resin into a salt type to regenerate the supporter, and (3) adsorbing fresh glucose isomerase on the regenerated supporter.
Claim 3
Dependent Claim
30 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said desorbing treatment is effected with an aqueous mineral acid and an aqueous alkali solution.
Claim 4
Dependent Claim
37 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said desorbing treatment is effected with an aqueous mineral acid and an aqueous mixture of an alkaline material and an electrolytic salt.
Claim 5
Dependent Claim
31 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said desorbing treatment is effected with an aqueous mineral acid and an aqueous electrolytic salt solution.
Claim 6
Dependent Claim
32 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said desorbing treatment is effected with an aqueous mixture of a mineral acid and an electrolytic salt.
Claim 7
Dependent Claim
29 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said desorbing treatment is effected at a temperature of from 50 to 70° C.
Claim 8
Dependent Claim
38 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said aqueous mineral acid is aqueous hydrochloric acid or sulfuric acid and said aqueous alkali solution is aqueous sodium hydroxide or potassium hydroxide.
Claim 9
Dependent Claim
46 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said aqueous mineral acid is aqueous hydrochloric acid or sulfuric acid and said aqueous mixture contains sodium hydroxide or potassium hydroxide and sodium chloride, potassium chloride, sodium sulfate or potassium sulfate.
Claim 10
Dependent Claim
43 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said aqueous mineral acid is aqueous hydrochloric acid or sulfuric acid and said aqueous electrolytic salt solution is aqueous sodium chloride, potassium chloride, sodium sulfate or potassium sulfate.
Claim 11
Dependent Claim
34 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein each of said aqueous mineral acid and said aqueous alkali solution has a concentration of from 0.2 to 2N.
Claim 12
Dependent Claim
47 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said aqueous mineral acid has a concentration of from 0.2 to 2N and said aqueous mixture has a molar ratio of said electrolytic salt to said alkali of from 20:1 to 1:2.
Claim 13
Dependent Claim
40 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said aqueous mineral acid has a concentration of from 0.2 to 2N and said aqueous electrolytic salt has a concentration of from 0.2 to 5M.
Claim 14
Dependent Claim
38 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said desorbing treatment is subjected to an insolubilized glucose isomerase which has been deactivated to its initial activity of from 15 to 50%.
Claim 15
Dependent Claim
46 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said treatment with an aqueous mineral acid occurs prior to said treatment with an aqueous alkali solution, an aqueous electrolytic salt solution and an aqueous mineral acid or a mixture thereof.
Claim 16
Dependent Claim
42 words
A process for the renewal of an insolubilized glucose isomerase according to claim 1, wherein said treatment with an aqueous alkali solution and an aqueous electrolytic salt solution or a mixture thereof, occurs prior to said treatment with an aqueous mineral acid.
References Cited (Backward Citations)
US3708397A
Cited
US4002576A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-09-11 | 1982-03-13 | 1982-09-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-09-11 | 1986-03-13 | 1986-09-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-09-11 | 1990-03-13 | 1990-09-12 | $7700.00 | Window Closed / Lapsed |