US4002576A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzyme carrier regeneration
Assignee: Corning Glass Works
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Filing: 1975-07-18
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Granted: 1977-01-11
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Expires: 1995-07-18
Abstract
Abstract Highly porous MgO-Al 2 O 3 particulate support materials useful for the immobilization of glucose isomerase can be regenerated for reuse by circulating a sodium hypochlorite solution through the particles thereby permitting in situ enzyme carrier regeneration.
Inventors
- 👤 Jerry L. Gregory
- 👤 Wayne H. Pitcher, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 06:23
CPC Classifications
C; C12; C12P; C12P1/00; B; B01; B01J; B01J20/00; B01J20/02; B01J20/04; B01J20/041; B01J20/06; B01J20/08; B01J20/30; B01J20/34; B01J20/3433; B01J20/345; B01J20/3475; C12N; C12N11/00; C12N11/14; C12P19/00; C12P19/24; C13; C13K; C13K11/00
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
82 words
A method of regenerating a spent immobilized glucose isomerase composite comprising glucose isomerase adsorbed to porous particles of a MgO-Al 2 O 3 carrier and having associated therewith carbonaceous material and contaminants resulting from use of the composite for the continuous isomerization of glucose to fructose, the method comprising the step of fluidizing the spent composite particles in a fluidized bed reactor by circulating a sodium hypochlorite solution through the composite under conditions sufficient to remove substantially all carbonaceous material and contaminants.
Claim 2
Independent Claim
82 words
A method of regenerating a spent immobilized glucose isomerase composite comprising glucose isomerase adsorbed to porous particles of a MgO-Al 2 O 3 carrier and having associated therewith carbonaceous material and contaminants resulting from use of the composite for the continuous isomerization of glucose to fructose, the method comprising the step of fluidizing the spent composite particles in a fluidized bed reactor by circulating a sodium hypochlorite solution through the composite under conditions sufficient to remove substantially all carbonaceous material and contaminants.
Claim 3
Dependent Claim
40 words
The method of claim 1 wherein the amount of NaOCl solution consists of an aqueous solution of at least about 5% by weight NaOCl, the amount of solution being about 5 ml per gram of support material to be regenerated.
Claim 4
Dependent Claim
16 words
The method of claim 1 wherein the regeneration step is for at least about 15 minutes.
Claim 5
Dependent Claim
48 words
The method of claim 1 wherein the regeneration step comprises circulating at least a 5% by weight aqueous NaOCl solution through the carrier particles contained in a fluidized bed reactor for at least about 15 minutes, the amount of solution being about 5 ml per gram of carrier.
Claim 6
Dependent Claim
59 words
The method of claim 1 wherein the support material comprises porous particles of a MgO-Al 2 O 3 composition, the amount of MgO ranging from about 0.84 to 12.0% by weight, the particles having an average pore diameter ranging from about 100 to 1000A and an average particle size ranging from about 4 to 200 mesh, U.S. Standard Sieve.
Claim 7
Dependent Claim
16 words
The method of claim 1 wherein the average pore size ranges from about 100 to 250A.
References Cited (Backward Citations)
US3671179A
Cited
US3894884A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-01-11 | 1980-07-12 | 1981-01-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-01-11 | 1984-07-12 | 1985-01-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-01-11 | 1988-07-12 | 1989-01-11 | $7700.00 | Window Closed / Lapsed |