Patent US4111785A successfully refreshed from Google Patents.
US4111785A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Apparatus for preparative electrophoresis
Assignee: Individual
•
Filing: 1977-05-16
•
Granted: 1978-09-05
•
Expires: 1997-05-16
Abstract
Abstract Electrophoresis apparatus comprises a tubular member for receiving a column of gel, and a plate-form body of solid but porous material, such as glass fit, defining an elution chamber at one end of the tubular member. An inlet duct delivers elution buffer into the plate-form body by way of one of the main faces thereof.
Inventors
- 👤 Willem Gerrit Roskam
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 15:01
CPC Classifications
C; C25; C25B; C25B7/00; G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
195 words
Electrophoresis apparatus, comprising a tubular member having first and second ends for receiving a column of gel, means defining an elution chamber at the first end of the tubular member, primary inlet duct means for passing buffer solution into the elution chamber, and outlet duct means for discharging buffer solution from the elution chamber and so disposed with respect to the primary inlet duct means that buffer solution passes from the primary inlet duct means to the outlet duct means substantially perpendicular to the central axis of the tubular member, the chamber-defining means comprising a plate-form body of solid but porous material having two main surfaces extending substantially perpendicular to the central axis of said tubular member, and the apparatus also comprising auxiliary inlet duct means for passing an auxiliary flow of buffer solution into the elution chamber through that main surface of the plate-form body which is further from the second end of the tubular member, and a layer of porous material between the elution chamber and the auxiliary inlet duct means, the porous material of said layer having a higher flow resistance to buffer solution than the porous material of the plate-form body.
Claim 2
Dependent Claim
195 words
Electrophoresis apparatus, comprising a tubular member having first and second ends for receiving a column of gel, means defining an elution chamber at the first end of the tubular member, primary inlet duct means for passing buffer solution into the elution chamber, and outlet duct means for discharging buffer solution from the elution chamber and so disposed with respect to the primary inlet duct means that buffer solution passes from the primary inlet duct means to the outlet duct means substantially perpendicular to the central axis of the tubular member, the chamber-defining means comprising a plate-form body of solid but porous material having two main surfaces extending substantially perpendicular to the central axis of said tubular member, and the apparatus also comprising auxiliary inlet duct means for passing an auxiliary flow of buffer solution into the elution chamber through that main surface of the plate-form body which is further from the second end of the tubular member, and a layer of porous material between the elution chamber and the auxiliary inlet duct means, the porous material of said layer having a higher flow resistance to buffer solution than the porous material of the plate-form body.
Claim 3
Dependent Claim
14 words
Apparatus as claimed in claim 1, wherein the plate-form body is a glass frit.
Claim 4
Dependent Claim
16 words
Apparatus as claimed in claim 2, wherein the layer of porous material is a glass frit.
Claim 5
Dependent Claim
19 words
Apparatus as claimed in claim 3, wherein the plate-form body and the layer of porous material are sintered together.
Claim 6
Dependent Claim
30 words
Apparatus as claimed in claim 1, wherein the plate-form body is sealed to said tubular member by means of a layer of vulcanized elastic material adhered to the plate-form body.
Claim 7
Dependent Claim
32 words
Apparatus as claimed in claim 1, wherein the plate-form body is sealed to said auxiliary inlet duct means by means of a layer of vulcanized elastic material adhered to the plate-form body.
Claim 8
Dependent Claim
41 words
Apparatus as claimed in claim 6, wherein the plate-form body and the auxiliary inlet duct means are fitted in a cylindrical housing member and the layer of vulcanized elastic material establishes a seal between the plate-form body and the housing member.
Claim 9
Dependent Claim
122 words
Electrophoresis apparatus, comprising a tubular member having first and second ends for receiving a column of gel, and means defining an elution chamber at the first end of the tubular member, the chamber-defining means comprising a plate-form body of solid but porous material having two main surfaces, one main surface of the plate-form body being directed towards the tubular member, and the apparatus also comprising duct means for delivering buffer solution into the elution chamber by way of the other main surface of the plate-form body, and a layer of porous material between the elution chamber and the duct means, the porous material of said layer having a higher flow resistance to buffer solution than the porous material of the plate-form body.
Claim 10
Dependent Claim
122 words
Electrophoresis apparatus, comprising a tubular member having first and second ends for receiving a column of gel, and means defining an elution chamber at the first end of the tubular member, the chamber-defining means comprising a plate-form body of solid but porous material having two main surfaces, one main surface of the plate-form body being directed towards the tubular member, and the apparatus also comprising duct means for delivering buffer solution into the elution chamber by way of the other main surface of the plate-form body, and a layer of porous material between the elution chamber and the duct means, the porous material of said layer having a higher flow resistance to buffer solution than the porous material of the plate-form body.
Claim 11
Dependent Claim
14 words
Apparatus as claimed in claim 8, wherein the plate-form body is a glass frit.
Claim 12
Dependent Claim
16 words
Apparatus as claimed in claim 9, wherein the layer of porous material is a glass frit.
Claim 13
Dependent Claim
19 words
Apparatus as claimed in claim 10, wherein the plate-form body and the layer of porous material are sintered together.
Claim 14
Dependent Claim
30 words
Apparatus as claimed in claim 9, wherein the plate-form body is sealed to said tubular member by means of a layer of vulcanized elastic material adhered to the plate-form body.
Claim 15
Dependent Claim
39 words
Apparatus as claimed in claim 11, wherein the plate-form body and the duct means are fitted in a cylindrical housing member and the layer of vulcanized elastic material establishes a seal between the plate-form body and the housing member.
Claim 16
Independent Claim
58 words
A method of constructing electrophoresis apparatus, wherein a layer of elastic material is applied to a plate-form body of solid but porous material, the elastic material is vulcanized, and the plate-form body is brought into contact with one end of a tubular member, the vulcanized elastic material establishing a seal between the plate-form body and the tubular member.
Claim 17
Independent Claim
58 words
A method of constructing electrophoresis apparatus, wherein a layer of elastic material is applied to a plate-form body of solid but porous material, the elastic material is vulcanized, and the plate-form body is brought into contact with one end of a tubular member, the vulcanized elastic material establishing a seal between the plate-form body and the tubular member.
Claim 18
Dependent Claim
66 words
A method as claimed in claim 13, wherein the plate-form body has two main surfaces, one of which is brought into contact with said one end of the tubular member and the other of which is brought into contact with duct means for delivering buffer solution into the plate-form body, and the vulcanized elastic material establishes a seal between the plate-form body and the duct means.
Claim 19
Dependent Claim
16 words
Apparatus as claimed in claim 1, wherein the tubular member is of circular internal cross section.
Claim 20
Dependent Claim
13 words
Apparatus as claimed in claim 6, wherein the elastic material is silicone rubber.
Claim 21
Dependent Claim
13 words
Apparatus as claimed in claim 7, wherein the elastic material is silicone rubber.
Claim 22
Dependent Claim
15 words
Apparatus as claimed in claim 8, wherein the housing member is of circular internal cross-section.
Claim 23
Dependent Claim
15 words
Apparatus as claimed in claim 9, wherein the tubular member is of circular internal cross-section.
Claim 24
Dependent Claim
13 words
Apparatus as claimed in claim 11, wherein the elastic material is silicone rubber.
Claim 25
Dependent Claim
15 words
Apparatus as claimed in claim 12, wherein the housing member is of circular internal cross-section.
Claim 26
Dependent Claim
14 words
A method as claimed in claim 13, wherein the elastic material is silicone rubber.
References Cited (Backward Citations)
US3375187A
Cited
US3533933A
Cited
US3579433A
Cited
US3704217A
Cited
US3697406A
Cited
US3791950A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-09-04 | 1982-03-06 | 1982-09-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-09-04 | 1986-03-06 | 1986-09-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-09-04 | 1990-03-06 | 1990-09-05 | $7700.00 | Window Closed / Lapsed |