US4107284A
Expired / Lapsed
US
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Radioimmunoassay procedure using a stabilized complex
Assignee: Individual
•
Filing: 1975-10-28
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Granted: 1978-08-15
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Expires: 1995-10-28
Abstract
Abstract An improved radioimmunoassay procedure involves the use of a stabilized complex of labelled antigen and antibody which has an extended shelf life as compared to the same complex absent the stabilizers. Since the time needed to incubate the mixture of labelled antigen and antibody is eliminated, the time for completing the assay is considerably shortened and simplified. The components for carrying out the procedure are packaged in a kit basically including standard antigen for generation of a standard curve, a stabilized labelled antigen-antibody complex and reference serum, if used. A plurality of stabilizers are used in the complex to provide a shelf life of six weeks or more.
Inventors
- 👤 Ishkhan V. Sultanian
- 👤 Joseph H. Irani
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 12:42
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/534; G01N33/74; G01N33/743; Y; Y10; Y10S; Y10S436/00; Y10S436/804
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
72 words
A stabilized radioactive isotope labelled antigen-antibody complex for use in radioimmunoassay procedures comprising: a mixture of a labelled antigen and an antibody specific to said antigen, said antigen being selected from the group consisting of triiodothyronine, thyroxine, thyroxine binding globulin, cortisol, estradiol, gentamicin and estrone, and a plurality of stabilizers present in said mixture in an amount effective to inhibit the labelled antigen from associating with protein other than said specific antibody.
Claim 2
Dependent Claim
72 words
A stabilized radioactive isotope labelled antigen-antibody complex for use in radioimmunoassay procedures comprising: a mixture of a labelled antigen and an antibody specific to said antigen, said antigen being selected from the group consisting of triiodothyronine, thyroxine, thyroxine binding globulin, cortisol, estradiol, gentamicin and estrone, and a plurality of stabilizers present in said mixture in an amount effective to inhibit the labelled antigen from associating with protein other than said specific antibody.
Claim 3
Dependent Claim
26 words
A stabilized complex as set forth in claim 1 wherein said stabilizers comprise the sodium salt of 8-anilino-1-naphthalene sulfonic acid, merthiolate, ferrous sulfate and N-N-dimethyl-p-phenylenediamine dihydrochloride.
Claim 4
Dependent Claim
43 words
A stabilized complex as set forth in claim 1 wherein said stabilizers are present in an amount of 200 microliters per between 10 milliliters and 25 milliliters of complex, and each said stabilizer having a concentration of between 0.1% to 2.0% by weight.
Claim 5
Dependent Claim
26 words
A stabilized complex as set forth in claim 1 wherein said radioactive isotope is selected from the group consisting of I 125 and H 3 .
Claim 6
Dependent Claim
45 words
A stabilized complex as set forth in claim 1 wherein said labelled antigen is selected from the group consisting of: I 125 labelled triiodothyronine, I 125 labelled thyroxine, H 3 labelled cortisol, H 3 labelled estradiol, H 3 labelled gentamicin, and H 3 labelled estrone.
Claim 7
Dependent Claim
136 words
A radioimmunoassay procedure for quantitatively determining the concentration of an antigen comprising the steps of: providing a stabilized radioactive isotope labelled antigen-antibody complex including a plurality of stabilizers present in an amount sufficient to provide a shelf life for said complex of between four to six weeks; admixing the antigen to be determined with said complex whereby a portion of the antigen to be determined becomes bound to the antibody of said complex, separating the complex and the antigen bound thereto from the unbound antigen, said antigen being selected from the group consisting of triiodothyronine, thyroxine, thyroxine binding globulin, cortisol, estradiol, gentamicin and estrone, and measuring the radioactivity at least of one of the bound or unbound antigen whereby the value of said radioactivity is a function of the quantity of the antigen to be determined.
Claim 8
Dependent Claim
136 words
A radioimmunoassay procedure for quantitatively determining the concentration of an antigen comprising the steps of: providing a stabilized radioactive isotope labelled antigen-antibody complex including a plurality of stabilizers present in an amount sufficient to provide a shelf life for said complex of between four to six weeks; admixing the antigen to be determined with said complex whereby a portion of the antigen to be determined becomes bound to the antibody of said complex, separating the complex and the antigen bound thereto from the unbound antigen, said antigen being selected from the group consisting of triiodothyronine, thyroxine, thyroxine binding globulin, cortisol, estradiol, gentamicin and estrone, and measuring the radioactivity at least of one of the bound or unbound antigen whereby the value of said radioactivity is a function of the quantity of the antigen to be determined.
Claim 9
Dependent Claim
22 words
A procedure as set forth in claim 6 wherein the quantity of the antigen is determined by reference to a standard curve.
Claim 10
Dependent Claim
17 words
A procedure as set forth in claim 6 wherein the radioactivity of the unbound antigen is measured.
Claim 11
Dependent Claim
25 words
A procedure as set forth in claim 6 wherein said stabilizers comprise the sodium salt of 8-anilino-1-naphthalene sulfonic acid, merthiolate, ferrous sulfate and N-N-dimethyl-p-phenylenediamine dihydrochloride.
Claim 12
Dependent Claim
25 words
A procedure as set forth in claim 6 wherein the radioactive isotope is selected from the group consisting of I 125 and H 3 .
References Cited (Backward Citations)
US3928553A
Cited
US3911096A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-08-14 | 1982-02-13 | 1982-08-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-08-14 | 1986-02-13 | 1986-08-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-08-14 | 1990-02-13 | 1990-08-15 | $7700.00 | Window Closed / Lapsed |