US3911096A
Expired / Lapsed
US
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Radioimmunoassay for measurement of thyroxine (T{HD 4{B ) and triiodothyonine (T{HD 3{B ) in blood serum
Assignee: Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center
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Filing: 1972-06-23
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Granted: 1975-10-07
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Expires: 1992-06-23
Abstract
Abstract This invention relates to a highly accurate, rapid and simple estimation of thyroxine (T4) directly from blood serum and also relates to the accurate measurement of triiodo-L-thyronine (T3) directly from blood serum. More specifically, the invention relates to a rapid, specific and reliable radioimmunoassay (RIA) technique for measurement of both T4 and T3 in unextracted serum. The method requires very small amounts of serum, e.g., 25 microliters ( Mu l) to measure T4 concentration in nearly all specimens representing clinical states of eu-, hypo- and hyperthyroidism, and 250 Mu l to measure T3 concentrations in specimens representing most clinical states.
Inventors
- 👤 Inder J Chopra
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:23
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/825; Y10S436/826
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
259 words
A METHOD OF MEASUREMENT OF THE CONCENTRATION OF A PARTICULAR THYROID HORMONE SELECTED FROM THE GROUP CONSISTING OF THYROXINE (T4) AND TRIODO-L-THYRONINE (T3) IN UNEXTRACTED HUMAN SERUM, WHICH COMPRISES: A: ADDING, TO A MEASURED QUANTITY OF UNEXTRACTED HUMAN SERUM, A. A BLOCKING AGENT IN AN AMOUNT SUFFICIENT TO DISPLACE ESSENTIALLY ALL OF SAID PARTICULAR THYROID HORMONE TO BE MEASURED FROM THYROXINE-BINDING GLOBULIN (TBG), B. BUFFERING IONS TO BUFFER SAID SERUM TO A PH OF BETWEEN ABOUT 6,8 TO ABOUT 9.6, C RADIOACTIVE THYROID HORMONE, OF THE TYPE TO BE MEASURED, IN AN AMOUNT WHICH WILL GIVE A MEASURABLE COUNTING RATE OF EITHER ANTIBODY BOUND OR FREE RADIOACTIVITY AFTER REACTION EQUILIBRIUM HAS BEEN REACHED AS SET FORTH IN STEP B BELOW, AND D. AN ANTIBODY IN SUFFICIENT QUANTITY TO BIND A SIGNIFICANT QUANTITY OF SAID RADIOACTIVE THYROID HORMONE IN THE ABSENCE OF ANY OF THE PARTICULAR NON-RADIOACTIVE THYROID HORMONE TO BE MEASURED, B: ALLOWING REACTION OF BOTH PARTICULAR THYROID HORMONE, TO BE MEASURED, AND SAID RADIOACTIVE THYROID HORMONE, WITH SAID ANTIBODY TO PROCEED SUBSTANTIALLY TO EQUILIBRIUM TO THEREBY PRODUCE ANTIBODY BOUND RADIOACTIVE THYROID HORMONE: C: SEPARATING SAID ANTIBODY BOUND RADIOACTIVE THYROID MONE, TO BE MEASURED FROM SAID FREE RADIOACTIVE THYROID HORMONE, D: MEASURING THE QUANTITY OF RADIOACTIVE THYROID HORMONE SELECTED FROM ANTIBODY BOUND RADIOACTIVE THYROID HORMONE AND FREE RADIOACTIVE THYROID HORMONE, E: PREPARING A STANDARD CURVE WITH KNOWN AMOUNTS OF THE PARTICULAR THYROID TO BE MEASURED, AND F: CORRELATING THE QUANTITY OF RADIOACTIVE THYROID HORMONE MEASURED WITH A KNOWN AMOUNT OF SAID PARTICULAR THYROID HORMONE READ FROM SAID STANDARD CURVE.
Claim 2
Independent Claim
259 words
A METHOD OF MEASUREMENT OF THE CONCENTRATION OF A PARTICULAR THYROID HORMONE SELECTED FROM THE GROUP CONSISTING OF THYROXINE (T4) AND TRIODO-L-THYRONINE (T3) IN UNEXTRACTED HUMAN SERUM, WHICH COMPRISES: A: ADDING, TO A MEASURED QUANTITY OF UNEXTRACTED HUMAN SERUM, A. A BLOCKING AGENT IN AN AMOUNT SUFFICIENT TO DISPLACE ESSENTIALLY ALL OF SAID PARTICULAR THYROID HORMONE TO BE MEASURED FROM THYROXINE-BINDING GLOBULIN (TBG), B. BUFFERING IONS TO BUFFER SAID SERUM TO A PH OF BETWEEN ABOUT 6,8 TO ABOUT 9.6, C RADIOACTIVE THYROID HORMONE, OF THE TYPE TO BE MEASURED, IN AN AMOUNT WHICH WILL GIVE A MEASURABLE COUNTING RATE OF EITHER ANTIBODY BOUND OR FREE RADIOACTIVITY AFTER REACTION EQUILIBRIUM HAS BEEN REACHED AS SET FORTH IN STEP B BELOW, AND D. AN ANTIBODY IN SUFFICIENT QUANTITY TO BIND A SIGNIFICANT QUANTITY OF SAID RADIOACTIVE THYROID HORMONE IN THE ABSENCE OF ANY OF THE PARTICULAR NON-RADIOACTIVE THYROID HORMONE TO BE MEASURED, B: ALLOWING REACTION OF BOTH PARTICULAR THYROID HORMONE, TO BE MEASURED, AND SAID RADIOACTIVE THYROID HORMONE, WITH SAID ANTIBODY TO PROCEED SUBSTANTIALLY TO EQUILIBRIUM TO THEREBY PRODUCE ANTIBODY BOUND RADIOACTIVE THYROID HORMONE: C: SEPARATING SAID ANTIBODY BOUND RADIOACTIVE THYROID MONE, TO BE MEASURED FROM SAID FREE RADIOACTIVE THYROID HORMONE, D: MEASURING THE QUANTITY OF RADIOACTIVE THYROID HORMONE SELECTED FROM ANTIBODY BOUND RADIOACTIVE THYROID HORMONE AND FREE RADIOACTIVE THYROID HORMONE, E: PREPARING A STANDARD CURVE WITH KNOWN AMOUNTS OF THE PARTICULAR THYROID TO BE MEASURED, AND F: CORRELATING THE QUANTITY OF RADIOACTIVE THYROID HORMONE MEASURED WITH A KNOWN AMOUNT OF SAID PARTICULAR THYROID HORMONE READ FROM SAID STANDARD CURVE.
Claim 3
Dependent Claim
39 words
The method of claim 1 wherein said thyroid hormone to be measured is thyroxine, said blocking agent is 8-anilino-1-napthalene disulfonic acid, said blocking agent being added to said human serum in quantities of at least about 4 ug/ul serum.
Claim 4
Dependent Claim
21 words
The method of claim 1 wherein said buffering ions are barbital ions, and said pH of said serum is about 8.6.
Claim 5
Dependent Claim
21 words
The method of claim 1 wherein said radioactive thyroid hormone is initially present in the order of 10,000 counts per minute.
Claim 6
Dependent Claim
25 words
The method of claim 1 wherein said antibody bound radioactive thyroid hormone after reaction, gives a counting rate of from about 2000-6000 counts per minute.
Claim 7
Dependent Claim
36 words
The method of claim 1 wherein said antibody bound radioactive thyroid hormone, to be measured, is separated from said free radioactive thyroid hormone by precipation of said antibody bound radioactive thyroid hormone with a second antibody.
Claim 8
Dependent Claim
35 words
The method of claim 1 wherein said antibody bound radioactive thyroid hormone, to be measured, is separated from said free radioactive thyroid hormone by precipation of said antibody bound radioactive thyroid hormone with gamma globulin.
Claim 9
Dependent Claim
35 words
The method of claim 1 wherein said antibody bound radioactive thyroid hormone, to be measured, is separated from said free radioactive thyroid hormone by precipation of said antibody bound radioactive thyroid hormone with charcoal suspension.
Claim 10
Dependent Claim
35 words
The method of claim 1 wherein said antibody bound radioactive thyroid hormone, to be measured, is separated from said free radioactive thyroid hormone by precipation of said antibody bound radioactive thyroid hormone with polyethylene glycol.
Claim 11
Dependent Claim
40 words
The method of claim 1 wherein said thyroid hormone to be measured is T4, said blocking agent is 2,7 naphthalene disulfonic acid, said blocking agent being added to said human serum in quantities of at least about 2 ug/ul serum.
Claim 12
Dependent Claim
41 words
The method of claim 1 wherein said thyroid hormone to be measured is T3, said blocking agent is 2,7 naphthalene disulfonic acid and said blocking agent is added to said human serum in quantities of at least about 1 ug/ul serum.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-10-06 | 1979-04-07 | 1979-10-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-10-06 | 1983-04-07 | 1983-10-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-10-06 | 1987-04-07 | 1987-10-07 | $7700.00 | Window Closed / Lapsed |