Patent US4106989A successfully refreshed from Google Patents.
US4106989A
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Thermostable cellulase and a method for producing the same
Assignee: Ajinomoto Co Inc
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Filing: 1977-04-26
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Granted: 1978-08-15
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Expires: 1997-04-26
Abstract
Abstract The present invention relates to a newly found thermostable cellulase of Sporotrichum cellulophilum and a method for producing the same.
Inventors
- 👤 Ichiro Komura
- 👤 Takeyoshi Awao
- 👤 Kazuhiko Yamada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 17:28
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; C12Y; C12Y302/00; C12Y302/01; C12Y302/01004; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
109 words
A cellulase produced by Sporotrichum cellulophilum and having the following properties: (a) hydrolyzes microcrystalline cellulose, hydrocellulose, sulfite pulp, filter paper, and cotton mainly to glucose and to a small amount of cellobiose; (b) optimum activity on Whatman No. 1 filter paper at pH 5.5 to 6.5 and at 60° C.; (c) optimum activity at 60° to 65° C.; (d) more than 50% of initial activity is retained at 50° C for 4 hours at any pH from 4.0 to 7.0; (e) stable when heated without any substrate at 50° C.; (f) retaining more than 40% of initial activity when heated at 80° C. for 1 hour without any substrate.
Claim 2
Dependent Claim
109 words
A cellulase produced by Sporotrichum cellulophilum and having the following properties: (a) hydrolyzes microcrystalline cellulose, hydrocellulose, sulfite pulp, filter paper, and cotton mainly to glucose and to a small amount of cellobiose; (b) optimum activity on Whatman No. 1 filter paper at pH 5.5 to 6.5 and at 60° C.; (c) optimum activity at 60° to 65° C.; (d) more than 50% of initial activity is retained at 50° C for 4 hours at any pH from 4.0 to 7.0; (e) stable when heated without any substrate at 50° C.; (f) retaining more than 40% of initial activity when heated at 80° C. for 1 hour without any substrate.
Claim 3
Dependent Claim
48 words
A method for producing the cellulase set forth in claim 1, which comprises culturing a strain of the fungus Sporotrichum cellulophilum capable of producing said cellulase in a culture medium until a substantial amount of said cellulase is accumulated in said culture medium, and recovering the accumulated cellulase.
Claim 4
Dependent Claim
19 words
A method as set forth in claim 2, wherein the said fungus is Sporotrichum cellulophilum FERM-P 3514 ATCC 20494.
Claim 5
Dependent Claim
32 words
A method as set forth in claim 2, wherein the said fungus is an artificially induced mutant of Sporotrichum cellulophilum which is capable of growing on a medium containing 2-deoxyglucose or 3-0-methylglucose.
Claim 6
Dependent Claim
19 words
A method as set forth in claim 4, wherein the said mutant is Sporotrichum cellulophilum FERM-P 3969 ATCC 20493.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-08-14 | 1982-02-13 | 1982-08-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-08-14 | 1986-02-13 | 1986-08-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-08-14 | 1990-02-13 | 1990-08-15 | $7700.00 | Window Closed / Lapsed |