Patent US4099918A successfully refreshed from Google Patents.
US4099918A
Expired / Lapsed
US
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Proxyl nitroxides as spin labels
Assignee: University of Oregon
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Filing: 1977-04-18
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Granted: 1978-07-11
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Expires: 1997-04-18
Abstract
Abstract Spectrometric analysis of biological molecules with tetraalkylpyrrolidine-N-oxyl nitroxides.
Inventors
- 👤 John F. W. Keana
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 15:03
CPC Classifications
G; G01; G01R; G01R33/00; G01R33/20; G01R33/60; G01R33/28; G01R33/281; Y; Y10; Y10T; Y10T436/00; Y10T436/24
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
86 words
In the spectrometric analysis of biological molecules, the method comprising labeling the molecule with a 2,2,5,5-tetraalkylpyrrolidine-N-oxyl nitroxide having the formula: ##STR8## wherein R 1 and R 2 are alkyl, aliphatic, or aromatic groups, saturated or unsaturated, and the carbon atoms in R 1 and R 2 , together with the carbon atom in the pyrrolidine-N-oxyl ring to which R 1 and R 2 are attached, form a carbon-to-carbon open chain of from four to fifty carbon atoms, and observing the ESR signal of the labeled molecule.
Claim 2
Dependent Claim
86 words
In the spectrometric analysis of biological molecules, the method comprising labeling the molecule with a 2,2,5,5-tetraalkylpyrrolidine-N-oxyl nitroxide having the formula: ##STR8## wherein R 1 and R 2 are alkyl, aliphatic, or aromatic groups, saturated or unsaturated, and the carbon atoms in R 1 and R 2 , together with the carbon atom in the pyrrolidine-N-oxyl ring to which R 1 and R 2 are attached, form a carbon-to-carbon open chain of from four to fifty carbon atoms, and observing the ESR signal of the labeled molecule.
Claim 3
Dependent Claim
13 words
The method of claim 1, wherein the nitroxide is a proxyl stearyl compound.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-07-10 | 1982-01-09 | 1982-07-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-07-10 | 1986-01-09 | 1986-07-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-07-10 | 1990-01-09 | 1990-07-11 | $7700.00 | Window Closed / Lapsed |